Labshake search
Citations for Bioline :
1451 - 1500 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplification (Bioline, Taunton, MA). The libraries were sequenced by multiplexing 8 libraries per lane on the Illumina HiSeq2500 sequencer ...
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bioRxiv - Molecular Biology 2020Quote: ... Sensifast Probe No-Rox mastermix (Bioline) or ImmoMix™ (Bioline)) ...
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bioRxiv - Molecular Biology 2020Quote: ... or ImmoMix™ (Bioline)) ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using the 2× SYBR Green PCR Master Mix (Bioline) with the CFX96 detection system and analyzed with CFX Manager softward (Bio-rad) ...
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bioRxiv - Immunology 2020Quote: ... 5 - 10 ng DNA was used for amplification with MyTaq Polymerase (Bioline). The PCR cycling conditions were as follows ...
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bioRxiv - Biochemistry 2020Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). ΔCt was calculated as Ct [Target]-Geometric mean (Ct [HPRT1] ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was carried out with 500 ng of RNA using the SensiFAST cDNA Synthesis Kit (Bioline, #BIO-65054) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... 2.5 μl of 2x SensiFAST SYBR Green Reaction Mix (Bioline Inc, Taunton, MA, USA), 0.2 μM of both reverse and forward primers and the PCRs were run on a Roche Lightcycler 480 thermocycler (Roche Applied Science ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of His3-SUMO-AscA (or His3-SUMO-AscAΔMBD) was induced using 1 mM isopropyl-thio-galactoside (IPTG; Bioline) overnight at 18°C ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.6ul reverse primer (5uM) and 10ul SensiFAST SYBR Hi-ROX mix (Bioline, Cat: BIO92020). The plate was then loaded into an Agilent AriaMax real time PCR machine (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... A CCDC26 exon 6 DNA template was generated by performing a PCR using Red Mix (Bioline, Cat. No. BIO25043) WT K562 cDNA and CCDC26-specific primers that incorporated a T7 RNA polymerase promoter at the 5’-end of the DNA strand to be later transcribed (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then used to generate the probe template by standard PCR using the MyTaq™ HSRED DNA Polymerase (Bioline) and opsin specific primers (listed in Table S2 ...
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bioRxiv - Cell Biology 2020Quote: ... Amplification reactions where performed using the SensiFAST SYBR No-ROX Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCRs were carried out using the SensiFAST SYBR No-ROX kit according to the manufacturer’s instructions (Bioline, London, United Kingdom) on a CFX96™ real-time instrument (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were digested at 4 °C on an orbital shaker-incubator at 200 × rpm (Bioline Global, New South Wales, Australia). After overnight digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... [SZ lab] or Bioscript (Bioline) [JLB lab] reverse transcriptase according to the manufacturer’s instructions with oligo-dT15 primer.
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bioRxiv - Zoology 2020Quote: RNA was reverse transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (BioLine) according to manufacturer’s specifications ...
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bioRxiv - Cell Biology 2020Quote: ... subsequently diluted in water (1:4) and mixed with SensiFAST SYBR Hi-Rox (Bioline) and the appropriate primers at a final concentration of 10 μM ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by qPCR quantification using SensiMix SYBR (Bioline, UK) and KAPA Illumina DNA standards (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... Hyperladder I (Bioline) was used as linear DNA size standard.
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bioRxiv - Pathology 2020Quote: ... lysed in Trisure® (Bioline Meridian Bioscience) using Direct-zol® RNA Purification Kit (Zymo Research ...
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bioRxiv - Pathology 2020Quote: Wheat curl mite DNA was extracted from single mites using the MyTaq™ Extract-PCR Kit (Bioline Meridian Bioscience, London, UK)) according to the manufacturer’s recommendations with the exception of the amount of starting material being less than 3 mg ...
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bioRxiv - Genetics 2020Quote: ... and 1 Unit MyTaqTM (Bioline; Meridian Bioscience Inc., London, UK) by incubation for 2 min at 96°C ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was done using 2X Taq-based Master Mix (Bioline) and TaqMan gene expression assays (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: RNA was isolated using the Isolate II RNA Mini Kit (Bioline BIO-52072), and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (Quantabio) ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... using SensiMix SYBR No-ROX (Bioline) and RPL30 primers provided in the kit to measure human DNA and Leishmania CS primers (Cysteine synthase ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... with a SensiMix SYBR No-ROX kit (Bioline); primer sequences available in Supplementary Table S1 ...
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bioRxiv - Cell Biology 2020Quote: 500 ng of RNA was reverse transcribed to DNA using SensiFast reverse transcriptase (Bioline) first-strand cDNA synthesis protocols ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... eggs were obtained from Bioline AgroSciences (France) ...
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bioRxiv - Pathology 2020Quote: ... were performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline). The final concentration of primers was 600nM and the probe concentration was 300nM ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were generated in 25 µl reactions containing 12.5 µl Bioline PCR Master Mix (Bioline USA Inc, Taunton, MA), 10.0 µl MG H2O ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was done using gene-specific primers in technical triplicates on a LightCycler 480 system using the Sensifast SYBR Master mix (Bioline). The ratio of experimental target mRNA to Actin control for each sample was calculated by Applied Biosystems software ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was made from 2 μg of RNA using the Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted 1 to 5 into RNAse free water to a total of 100 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were carried out with ∼100 ng genomic DNA in MyTaq Red mix (Bioline) and purified using the PCR Isolate II PCR and Gel Kit (Bioline ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were collected and genomic DNA was extracted using the ISOLATE II Genomic DNA kit (Bioline cat. no. BIO-52067). PCR was performed in two steps and pooled experiments were performed in triplicates for a higher coverage ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified PCR product was run on a 1% agarose gel cut out to remove remaining primer dimers and cleaned with PCR Isolate II PCR and Gel Kit (Bioline). The purified libraries were sequenced on an Illumina HiSeq2500 or MiSeq depending on the expected complexity of the library.
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bioRxiv - Molecular Biology 2020Quote: ... Both PCR reactions were carried out with 25 µl MyTaq Red mix (Bioline cat. no. BIO-25044), 0.5 µM of each primer and 50 µl final volume ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR using MyTaq DNA polymerase (Bioline).
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 100 mg of grounded tissue per sample was homogenized in 1 mL of the commercial reagent TRIsure (Bioline), and total RNA was extracted following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... amplification was performed using SensiFAST™ SYBR® & Fluorescein Kit (Bioline) and iQ5 Biorad system ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed with 500ng of cDNA and the SensiFast™ SYBR® No-ROX kit (Bioline) and a Lightcycler® 480/1536 (Roche ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or SensiFAST™ SYBR® No-ROX Supermix (# BIO-980050, Bioline, London, UK) and run on a CFX96 or CFX394 (Biorad) ...
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bioRxiv - Molecular Biology 2020Quote: ... with SensiFAST SYBR Lo-ROX Kit (Bioline). Cycling parameters were 95°C for 3 min ...
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bioRxiv - Microbiology 2020Quote: ... using thermal cycling conditions according to the SensiFAST protocol (Bioline). The hfq gene was used as an internal standard for normalization as its expression is constitutive (and unaffected by LuxR ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized using Tetro Reverse Transcriptase (Bioline). qRT-PCRs were performed with three technical and three biological replicates ...
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bioRxiv - Plant Biology 2020Quote: ... ‘Kitaake’ using a high fidelity Taq DNA polymerase (Bioline, VELOCITY DNA Polymerase) and cloned into entry vector pDONR221 (Addgene ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We obtained the bees from a commercial supplier (Syngenta Bioline, Weert, The Netherlands) and tagged them with Opalith number tags (Christian Graze KG ...
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bioRxiv - Pathology 2020Quote: ... using SensiMixTM SYBR® Green (Bioline, London, UK) in a 7900HT Real-Time PCR sequence detection system (Applied Biosystems ...