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Citations for Bioline :
1351 - 1400 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... run-on products labelled with BrUTP were extracted with TRIzure (Bioline, London, UK). RNA was precipitated first for 30 min on RT and then for an extra 10 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: The assay consists of 10 μl of reaction volume including 5 ul of 2X buffer (Bioline, Bio-11060), 2 ul of Primer/probe mix in 1xTE with final concentration of 100 nM-600 nM of primers and 50 nM – 500 nM of probes ...
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bioRxiv - Cancer Biology 2020Quote: ... and SensiMix SYBR No-ROX Kit™ (Bioline, QT650-20), using RPLP0 as a reference gene ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumors were first lysed with Trisure reagent (Bioline) and RNA was further purified with the Nucleospin RNA II Kit (Macherey-Nagel) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quantitative PCR was carried out using CTS gene specific primer pairs (Supplemental Data 8) with SYBR green-based visualization of product amplification (Bioline). Changes in CTS gene transcript levels relative to Actin (ACT1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.16 μl dNTPs (each 25 mM) and 0.1 μl Taq (Bioline 5U/μl), made up to 20 μl with autoclaved MilliQ water ...
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bioRxiv - Biochemistry 2020Quote: ... using a SensiMix(tm) (BioLine, London, UK) protocol for SYBR green reactions ...
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bioRxiv - Microbiology 2020Quote: cDNA was synthesized from high integrity RNA samples using the tetro cDNA synthesis kit (Bioline). The SensiFAST SYBR Hi-ROX kit (Bioline ...
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bioRxiv - Plant Biology 2020Quote: ... 3-day-old etiolated hypocotyls or roots were mounted in water under a pad of 0.8% agarose (Bioline). To limit the time that seedlings spent mounted ...
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bioRxiv - Microbiology 2020Quote: ... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015 ...
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bioRxiv - Microbiology 2020Quote: ... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015; and after 2015, SD Bioline dengue duo (dengue NS1 Ag+ IgG/IgM), Korea ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR reactions using cDNA samples as template were carried out using a SensiFAST SYBR No-ROX Kit (Bioline) in a CFX96 Touch (BIO-RAD ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed with BIOTAQ DNA Polymerase (Bioline, UK) using the following program ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions (Bioline Reagents Ltd). The primers used for lidations were as follow ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was used for reverse transcription according to the protocol of the SensiFASTtm cDNA synthesis kit from Bioline. PCR reactions were carried out with 5 μL of 100x diluted cDNA solution ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative Real-Time PCR was performed using a SensiFAST™ SYBR® No-ROX Kit (Bioline; www.bioline.com). Fluorescence was monitored with a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: Gene and promoter amplification were performed using MyFi™ DNA Polymerase (Bioline). FvMYB10-gypsy and Fvb1 FIN12 genomic fragments flanking the deleted region were both amplified with 5Prime PCR Extender System (5Prime ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 μL of the obtained cDNA was used for real-time PCR with SYBR™ green master mix (Bioline, Luckenwalde, Germany) on a C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR reactions were performed in a 384-well microlitre using SensiFAST SYBR® No-ROX Kit (Bioline). The primers used here were designed using the open-source program QuantPrime-qPCR primer designed tool (Arvidsson et al. ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT PCR was performed using the SensiFAST™ Probe Lo-ROX one-step kit (Bioline). In each reaction the primers final concentration was 600nM and the probe concentration was 300nM ...
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bioRxiv - Microbiology 2020Quote: ... prat2 expression was assessed with the SensiFAST™ SYBR® Hi-ROX One-Step Kit (Bioline) according to manufacturer’s recommendations with specific primers PP25361 (Forward ...
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bioRxiv - Microbiology 2020Quote: ... and then transformed into ElectroSHOX cells (Bioline USA, discontinued). Following transformation ...
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bioRxiv - Microbiology 2020Quote: ... The full-length genome or the nsp14-coding region were amplified by PCR using MyTaq DNA polymerase (Bioline) and after purification the PCR product was sequenced by Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR primer concentration was 500 nM in a volume of 10 μl with the SensiFast SYBR No-ROX Kit (Bioline, London, UK). The following amplification program was used ...
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bioRxiv - Microbiology 2020Quote: ... we used a primer concentration of 500 nM in a final volume of 10 μl with the SensiFast SYBR No-ROX Kit (Bioline, London, UK). The amplification conditions were as follows ...
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bioRxiv - Microbiology 2020Quote: ... Influenza virus M gene copy number per μL RNA was determined by qRT-PCR using the SensiFAST Probe Lo-ROX One-Step qRT-PCR System Kit (Bioline) on the ABI 7500 Real Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... either directly after purification with SureClean (Bioline) or after cloning them in pGEMTeasy (Promega)
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR or RT-PCR analyses were performed using Brilliant III SYBR green QPCR master mix (Bioline) with gene-specific primers on a Applied Bioscience StepOnePlus qPCR machine (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... The SensiFAST SYBR Hi-ROX kit (Bioline) was used for qPCR reactions ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 µL SensiFAST(tm) Probe Hi-ROX Kit master mix (Bioline, Tauton, MA), 1.25 µL each of Cx ...
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bioRxiv - Neuroscience 2020Quote: ... Each PCR reaction (20 μL) contained 2X SensiFAST SYBR® No-ROX mix (Bioline, BIO-98005, 10 μL), 10μM primers ...
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bioRxiv - Neuroscience 2020Quote: ... 10-50 ng of RNA from brain samples or 10-25 ng of RNA from cell body and neurite preparations of neuron cultures was reverse transcribed using Sensifast (Bioline, TN). For the neuron cultures ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 mM MgCl (Bioline, Boston, MA), 1 mM dNTP (Bioline ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR (qPCR) was carried out using the cDNA generated previously employing the SensiFAST SYBR mix No-Rox Kit (Bioline, UK in a Rotor-Gene 6000 real-time PCR cycler (Corbett Research ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA products were diluted 10 times and used for qPCR using a StepOnePlusTM instrument (Applied Biosystem, USA) and SensiFAST SYBR Hi-ROX Kit (Bioline) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with 50 ng of cDNA in each well and SensiFAST probe no-ROX mix (Bioline) using a CFX Connect system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA was extracted using the ISOLATE II Genomic DNA kit (Bioline cat. no. BIO-52067) and DNA was processed for high-throughput sequencing similar to conventual DamID (Vogel et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the PCR Isolate II PCR and Gel Kit (Bioline) or by ExoSAP (for primers see Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: ... with 40 µl MyTaq HS Red mix (Bioline, BIO- 25048) and 0.5 µM of each primer ...
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bioRxiv - Molecular Biology 2020Quote: ... buffer with 1 mg/ml Proteinase K (Bioline, Cat. No. BIO-37084) to pre-lyse the cells ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was prepared from 300 ng of RNA in a total reaction volume of 20 µl using the Sensi-FAST cDNA synthesis kit (Bioline, BIO-65053). RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was isolated using the ISOLATE II RNA Mini Kit (Bioline, BIO-52072). cDNA was prepared from 300 ng of RNA in a total reaction volume of 20 µl using the Sensi-FAST cDNA synthesis kit (Bioline ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and 1 µl of cDNA per reaction in a total volume of 10 µl ...
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bioRxiv - Neuroscience 2020Quote: PCR products were generated with BIOMIX red (BIOLINE, London, UK) and 1 μl of the respective restriction enzyme was added directly to the final PCR product for RFLP analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μl of digest were run on 2 % agarose gel (BIOLINE, London, UK) and imaged on a trans illuminator ...
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bioRxiv - Cell Biology 2020Quote: ... and Velocity polymerase (Bioline). We note that the Phusion polymerase using the buffer provided by the manufacturer does not work for PCR cassette amplification with M1 and M2 tagging oligos ...
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bioRxiv - Cell Biology 2020Quote: 5.0 µL of dNTPs (10 mM stock, Bioline, BIO-39026)
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bioRxiv - Cell Biology 2020Quote: ... 4 were generated from 200 ng genomic DNA (gDNA) using junction-specific primers (Table S4) by a two-step nested PCR with Velocity polymerase (Bioline). The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter) ...