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Citations for Bioline :
701 - 750 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... separated on agarose gels and extracted using a Bioline Isolate II kit (Bioline, Taunton, MA). The amplified StEP library was then cloned into an expression vector containing a C-terminal mCherry instead of a muGFP fusion tag and transformed E ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression levels of target genes were determined by RT-PCR using SensiMix SYBR No-Rox (Bioline) with the primers shown in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted from MSCs and OA-HSFs using the Isolate II RNA Micro Kit (Bioline, Canada) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and RNase inhibitor (10 U per reaction, Bioline). The resulting cDNA was subjected to real-time PCR in a Rotorgene 6000 (Corbett Life Science ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cDNA was subjected to real-time PCR in a Rotorgene 6000 (Corbett Life Science, New South Wales, Australia) using Immomix (2× dilution; Bioline), SYBR Green I (10,000× dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... and SensiFAST cDNA Synthesis Kit (Bioline), respectively ...
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bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Developmental Biology 2022Quote: RNA was isolated from cells on with the Isolate II RNA Mini Kit (Bioline, BIO-52702). 1 μg was converted to cDNA with qScript (Quanta ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed using the SensiFAST Sybr Low Rox kit (Bioline) with specific primers listed in Table S2 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed the desired deletions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed deletions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCRs were run using the SensiFAST probe No-ROX kit (Bioline, Cat#BIO-86005) on a Roche Life Science LightCycler 384 ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit, Bioline) with a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 750 ng total RNA was reverse transcribed to form cDNA according to the manufacturer’s protocol (SensiFAST™ cDNA Synthesis Kit, Bioline, Luckenwalde, Germany). Real-time PCR analyses were performed on a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... employing SYBR Green chemistry (SensiMix™ SYBR® & Fluorescein Kit, Bioline). PCR reactions contained 2.0 μL diluted cDNA sample (corresponding to 7.5 ng total RNA ...
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bioRxiv - Cell Biology 2022Quote: ... 750 ng total RNA was reverse transcribed to form cDNA according to the manufacturer’s protocol (SensiFAST™ cDNA Synthesis Kit, Bioline, Luckenwalde, Germany). Real-time PCR analysis was performed according to the manufacturer’s protocol (SensiMix™ SYBR® & Fluorescein Kit ...
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bioRxiv - Developmental Biology 2022Quote: ... purified using ISOLATE II PCR and Gel Kit (Bioline, Australia) and Sanger sequenced at the Australian Genome Resource Facility (AGRF) ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA extraction was performed using the ISOLATE II RNA Plant Kit (Bioline, Australia), followed by TURBOTM DNase treatment (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Each reaction contained 5 μl SensiFAST 1x LoRox SYBR Mix (Bioline), 0.25 μl forward primer (10 μM) ...
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bioRxiv - Genomics 2022Quote: ... Quantification was carried using SensiFAST™ SYBR® Hi-ROX One-Step Kit (Bioline). Absolute RNA quantification was obtained by interpolating its CT value against the standard curve ...
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bioRxiv - Genomics 2022Quote: ... meristems were snap-frozen and total RNA was extracted using Isolate II RNA plant kit (Bioline, UK). RNA-sequencing libraries were synthesised for each sample using NEBNext Ultra II stranded RNA library synthesis kits ...
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bioRxiv - Genomics 2022Quote: ... 12.5 µl of MyTaq Hot Start Red Mix (Bioline), and 10.5 µl of dH2O ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qPCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Genomics 2022Quote: ... 25µL Bioline Myfi mix (Bioline GmbH, Germany), 2µL of forward and reverse primers (10μM) ...
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bioRxiv - Genetics 2022Quote: The full exon 3 of PRNP gene (771 bp) of all animals was amplified by PCR using MyTaq™ Red Mix (Bioline Meridian Life Sciences-BIO-25043). Briefly ...
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bioRxiv - Genetics 2022Quote: PCR reactions were routinely performed with VELOCITY DNA Polymerase (Bioline, London, UK) using an MJ Mini Personal Thermal Cycler (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: PCRs were performed using MyTaq HS DNA Polymerase (Bioline). Reaction mixes contained 5 μl 5x MyTaq Reaction Buffer ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of RNA was then reverse transcribed with random hexamer and anchored oligo dT primers using SensiFast cDNA synthesis kit (Bioline). Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was performed directly from the lysates with region targeting primers (Supplementary Table 2) and MyTaq Red Mix (Bioline) followed by gel electrophoresis to reveal biallelic knockout clones ...
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline) as per manufacturers’ protocol with β-actin acting as an endogenous reference ...
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bioRxiv - Genetics 2022Quote: The frequency of proximity ligated fragments containing the Myc promoter and other downstream regions was then quantified on the BioRad C1000 Thermo cycler using the SensiMix SYBR NOROX kit (Bioline) with 60°C annealing temperature ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Genetics 2022Quote: ... Complementary DNA (cDNA) was synthesised using the Tetro cDNA synthesis kit (Bioline). qRT-PCR of ISGs (Ifit1 and Irf7 ...
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bioRxiv - Genetics 2022Quote: ... Frozen tissues were homogenised in Trisure reagent (Bioline) using IKA T10 basic S5 Ultra-turrax Disperser ...
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bioRxiv - Genetics 2022Quote: ... while qPCR reactions - with SensiFAST™ SYBR® No-ROX Kit (Bioline). For oligonucleotide sequences see Table S1.
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bioRxiv - Plant Biology 2022Quote: ... RNA was extracted with the use of TRIsure reagent (Bioline), and 500 ng of which was used for reverse transcription using SuperScript IV (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were purified from agarose gels using the ISOLATE II PCR and Gel Kit (Bioline, Memphis, TN, USA), and quantified in a NanoDrop One C Spectrophotometer (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 100 μg/ml ampicillin were processed with either the ISOLATE II Plasmid Mini Kit (Bioline, #BIO-52057) for sequence verification ...
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bioRxiv - Synthetic Biology 2022Quote: ... Amplicons of the appropriate size range for the transgene of interest were then gel extracted for nanopore sequencing using the ISOLATE II PCR and Gel Kit (Bioline, #BIO-52060) (Figure S5).
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were generated by PCR amplification of sequences of interest with Velocity DNA Polymerase (Bioline, #BIO-21099) using primers synthesized by IDT or restriction enzyme digestion with NEB High-Fidelity enzymes ...
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bioRxiv - Plant Biology 2022Quote: ... the SensiFAST SYBR No-ROX Kit was used according to manufacturer’s recommendations (Bioline Reagents Ltd., London, UK).
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bioRxiv - Molecular Biology 2022Quote: ... Amplifications were carried-out in total volume of 10 µL containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of each primer set (dhfr-F/R and 529rpe-F/R) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed in a total reaction mixture of 10 μL containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of reverse and forward primers ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
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bioRxiv - Physiology 2022Quote: ... and a rapid diagnostic test for malaria (RDT; SD BIOLINE Malaria Ag P.f©, Abbott) using immunochromatographic detection of Histidine Rich Protein 2 (HRP2 ...
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bioRxiv - Physiology 2022Quote: ... All primer pairs were evaluated for specificity and to ensure that they produced only a single band of the appropriate length using MyTaq DNA Polymerase (Bioline, Alexandria, NSW, Australia) and agarose gel electrophoresis.
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the SensiFAST SYBR No-ROX kit (Bioline) according to the manufacturer’s instructions on a CFX96 Touch real-time PCR instrument (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of SensiFAST™ SYBR® & Fluorescein mix (Bioline), sterile DNA-free water and 5 ng of sample DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was recovered using a DNA purification kit (Bioline #52060). The binding levels of Gal4 and TBP were determined using qPCR ...