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Citations for GE Life Sciences :
2851 - 2900 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 10 ug were separated on an 0.8% agarose gel and transferred to a Nylon membrane (GE Healthcare Limited, Buckinghamshire, England) as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... Analytical size exclusion chromatography using a Superdex 200 Increase 10/300 column confirmed the absence of aggregates from the protein preparation (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... and fractionated by linear gradient with end buffer (10 mM Tris-HCl, 1 M NaCl) using an AKTAprime plus (GE Healthcare) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Sample volumes of 100 μl of APT1 WT and ATP2 WT at 40 μM were injected into a Superdex 75 10/300 GL column (GE Healthcare) previously equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer C with 0.005% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... concentrated to < 1 mL by centrifugal ultrafiltration (10,000 or 5,000 MWCO ultrafilter) and loaded onto a Superdex 75 Increase (10/300 GL) column (GE Healthcare Life Sciences) equilibrated in storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The eluates were subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... A2AAR reconstituted into nanodiscs was subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove soluble aggregates ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: Size-exclusion chromatography was carried out using a Superdex 75 (HR 10/30) coupled to an Äkta purifier system (GE Healthcare), equilibrated with a 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions were concentrated using a 50-kDa MWCO concentrator (Amicon) and further purified Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) in a buffer containing 25 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... but no 3C protease cleavage was performed and the protein was further purified on a Superdex 200 Increase 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... The eluted mutant was then applied to a Superdex 200 10/300 GL size-exclusion chromatography column (Cytiva, Tokyo, Japan (GE Healthcare)) to isolate the monodisperse fraction of the AaRseP mutant ...
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bioRxiv - Biophysics 2021Quote: ... 100-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 200 or 75 10/300 GL Increase size-exclusion chromatography column (GE Healthcare) in 20 mM Tris HCl ...
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bioRxiv - Biophysics 2020Quote: ... Plexins and GTPases were further purified by either size exclusion chromatography or using a PD-10 Sephadex G-25 desalting column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the pH of the aSyn sample was adjusted to pH 7 and filtered through a 0.22 μm filter before being loaded onto a HiPrep Phenyl FF 16/10 (High Sub) column (GE Healthcare, Sweden) and eluted in HIC buffer A (50 mM Bis-Tris ...
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bioRxiv - Biophysics 2021Quote: ... Insoluble material was removed by ultracentrifugation at 150,000 x g for 20 min before purification by SEC on a Superpose 6 increase 10/300 GL column (GE Healthcare) equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... an equal amount of protein sample (between 10-20 μg) was separated by SDS-PAGE and then transferred onto a nitrocellulose membrane (Hybond, GE Healthcare) for two hours at 250 mA on ice ...
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bioRxiv - Cell Biology 2020Quote: ... The individual TMT labeled samples were pooled and then fractionated using strong cation exchange chromatography on an AKTA Pure 10 (GE Healthcare) equipped with a Luna 5 μm 100 angstrom 150 x 2.1 mm strong cation exchange (SCX ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tag was cleaved with Prescission protease overnight and removed by molecular sieving using an analytical Superdex 75 10/300 chromatography column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer A (devoid of ATP and PefaBloc but containing 200 mM instead of 30 mM imidazole) and further purified over a Superdex 200 10/300 GL column (GE Healthcare) in buffer B (25 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: 10 μM purified WIPI2d was mixed with 20 μM of GST or GST-ATG16L1(207-230) and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 25mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein eluted with a gradient from 20 to 500 mM imidazole over 10 column volumes using an AKTA Pure (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... 100 µL MkeTIR-SAVED (at least 100 µM) were injected into a size exclusion column (Superose 6 Increase 10/300 GL or Superose 12, GE Healthcare) equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... the flow-through containing Fab fragments was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... by inoculating cre+-Raptorfl/fl mice with 10 mg/kg TLR3 agonist poly(I:C) once every two days as indicated (GE Healthcare, WI) to induce Raptor cKO in the mouse ...
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bioRxiv - Biochemistry 2022Quote: ... of GlOCPX or SynOCP1 samples (5 mg/ml in 50 μl) was performed on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a Prostar 335 detector (Varian ...
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bioRxiv - Molecular Biology 2022Quote: ... to approximately 3 – 4 ml and further purified by size-exclusion chromatography at 18 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare). His-TEV-CobB ...
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bioRxiv - Biochemistry 2022Quote: ... The flow-through was concentrated to ∼3mg/mL (100kDa MWCO) and subjected to size exclusion chromatography on Superdex 200 increase 10/300 column (GE healthcare) in size exclusion buffer containing 50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration was adjusted to 250 mM KCl and the protein was loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... and the excess of dye was removed by using a PD Minitrap G-25 column for NPM or a Superdex 200 10/300 GL (GE Healthcare) size exclusion chromatography column for SET/TAF-Iβ ...
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bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... Gel filtration chromatography was performed as described in (Iwai et al., 2015) using the ÄKTAmicro chromatography system with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: Size-exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) was carried out using a Superdex 200 Increase 10/300 column (GE Healthcare) and a combination of a UV-Vis Prostar 335 (Varian ...
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bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 10 ml Strep-Tactin column (Iba) and a gel filtration column (Superdex200 16 600, GE Healthcare). The purified protein was stored in PBS containing 1 mM TCEP at -80°C ...
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bioRxiv - Immunology 2022Quote: ... FoxP2ΔN and His6-GST fused Runx1 (residues 371-451) were purified by a combination of chromatography on Ni-NTA agarose and Superdex 200 Increase 10/300 (GE Healthcare) columns ...
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by size-exclusion chromatography on Superdex 75 Increase 10/300 (GE Healthcare) column ...
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bioRxiv - Microbiology 2022Quote: ... or His-NTV-AviTag (80 µl at 2 mg/ml) were injected on a Superdex 200 increase 10/300 GL column (GE Healthcare), equilibrated at 4°C with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and injected onto a Superdex200 10/300 GL column (GE Healthcare) equilibrated in the buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The final protein purification step and determination of oligomeric state was done on a Superose6 Increase 10/300 size exclusion column (GE Healthcare). The column was equilibrated in buffer B and the concentrated protein was loaded onto the column ...