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Citations for GE Life Sciences :
3051 - 3100 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... fused to lysozyme and BRD4-ET either alone or together was evaluated by injecting each purified protein onto a Superdex 75 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with 20 mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing Mre11 protein were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in Mre11 SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... eluted fraction were set aside after the first step of Ni affinity chromatography and directly loaded on a Superdex 200 Increase (10/300 GL) column (GE Healthcare) and eluted in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fraction was concentrated and further purified by size exclusion chromatography using an S200 increase (10/300 GL) column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane (GE life sciences, 1060002) using a Pierce™ Power Station (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... The target proteins flowed through from the column were collected and further purified by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) with lysis buffer containing 5 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted supercomplex solution was concentrated as described above and further purified by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare), preequilibrated with buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Oligomerization of delVirB4_CTn4 and delVirD4_CTn4 proteins was studied by gel chromatography on Superdex200 10/30 GL column connected to ÄKTA Explorer liquid chromatography system (GE Healthcare). The proteins ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Biophysics 2021Quote: ... Purified TolC and colicin E1-T or -TR were mixed at a 1:2 molar ratio to ensure an excess of colicin to saturate TolC binding sites and incubated at room temperature for 1 hour before loading onto a Superdex 200 Increase 10/300 GL column (GE Healthcare). The protein was eluted with 1.5 column volumes into 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The LFT-μOR-Gi1-scFv16 complex was further purified by size exclusion chromatography on a Superdex 200 10/300 GL column (GE healthcare) in 20 mM HEPES 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... purified fluorescent ULK1 complex (FIP200-MBP/EGFP-ATG13/EGFP-ATG101/ULK1) was mixed with purified GST-NDP52 or GST-4xUb and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences) coupled to an Äkta purifier system (GE Healthcare). To perform the subsequent experiments ...
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bioRxiv - Biophysics 2021Quote: ... Fractions showing the corresponding encapsulin band on SDS-PAGE were pooled and subjected to further purification via anion-exchange chromatography using a HiPrep Q 16/10 column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Nap1 containing fractions were concentrated to 2 mg/mL and loaded on a Superdex 200 10/300 GL gel-filtration column (GE Healthcare) pre-equilibrated with 25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing purified FliF RBM2 (FliF124-229) were further purified by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare) in a buffer containing 50 mM Hepes pH 9.0 ...
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bioRxiv - Microbiology 2021Quote: ... the released proteins were further purified on a Superdex 200 (for nsp12) or a Superdex 75 (for nsp7, nsp8 and NiRAN) Increase 10/300 GL column (GE Healthcare) using 25mM HEPES–NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... size exclusion chromatography (SEC) coupled to MALS analysis was performed on MiniDAWN TREOS II coupled to S200 10/300 increase column (GE Healthcare) mounted on FPLC system (AKTA purifier) ...
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bioRxiv - Biophysics 2021Quote: ... using a Shimadzu HPLC LC-20AD/RF-20A (488 nmex, 512 nmem) instrument and Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... These solutions were prepared either by dialysis against the buffer at 4°C or by passage through PD-10 desalting columns (GE Healthcare). Protein concentrations were measured spectrophotometrically using known values for the extinction coefficient ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare). Immunoprecipitation was performed overnight by incubation with anti-V5 antibody ...
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bioRxiv - Biophysics 2020Quote: Proteins were further purified by size-exclusion chromatography (Superdex 200 HiLoad 26/60 or Superdex S200 Increase 10/300 GL; GE Healthcare) on an AKTA pure fast protein liquid chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: Fab and scFv constructs were eluted from affinity purification and then purified further via size exclusion chromatography using the Superdex 200 Increase 10/300 GL column (GE HealthCare) and 1X PBS ...
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bioRxiv - Microbiology 2020Quote: The Vag8:C1-INH complex was prepared in vitro by incubating C1-INH in ∼1.5 molar excess with Vag8 at room temperature for 10 min followed by purification using size exclusion chromatography on a S200pg 16/600 column (GE Healthcare). The eluted fractions were analysed by SDS-PAGE followed by ultrafiltration to concentrate the protein complex.
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bioRxiv - Microbiology 2020Quote: ... Germany) and differentiated into macrophages by culture for 7 days in RPMI1640 medium supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300, bead volume: 24 ml; GE Healthcare), also equilibrated with WB ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the amphipol associated complex was controlled by performing an additional size exclusion step on a Superdex 200 increase column (10/300, bead volume: 24 ml; GE Healthcare).
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bioRxiv - Immunology 2020Quote: The GST-rNLRP1FIIND S969A-rDPP9 complex and rNLRP1FIIND proteins purified as described above were subjected to gel filtration (Superose 6, 10/30; GE Healthcare) in buffer containing 10 mM Tris pH 8.0 and 100 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Properly folded trimers were eluted with 3M MgCl2 and immediately dialyzed into storage buffer before being subjected to multiple size exclusion chromatography runs with a Superdex200 16/60 column followed by a Superose6 10/300 column (GE Healthcare). Peak fractions verified to be BG505 SOSIP.664 trimers were stored as individual fractions at 4°C in storage buffer.
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bioRxiv - Microbiology 2020Quote: ... fluorescently labeled R18-HRSV or culture supernatants of HEp-2 were separated from excess R18 by a separation column (PD-10 Desalting Columns GE Healthcare). HRSV-R18 or culture supernatants of HEp-2 were incubated in suspension with A3.01 and HEp-2 cells at 4°C for 1 h to allow virus attachment ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were concentrated using Ultra-15 Centrifugal Filter Units (Amicon) and further purified by using a Superdex 75 Increase 10/300 GL (GE Healthcare) size exclusion column in TBS (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The glutathione Sepharose column eluate was then concentrated to 500 μL and passed over an S75 10/300 size exclusion column (GE healthcare) with 20 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare). Protein standards from Bio-Rad were used to interprete elution profiles ...
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bioRxiv - Immunology 2020Quote: ... The sample was concentrated using Vivaspin-20 10 kDa and further purified via SEC using ÄKTA Pure 25 (GE Life Sciences) equipped with Superdex 75pg 16-600 column (GE Life Sciences) ...
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bioRxiv - Immunology 2020Quote: ... concentrated and dialyzed into 1x PBS buffer before being further purified by size exclusion chromatography (SEC) using Superdex 200 10/300 GL column (GE healthcare). Identified SEC eluate fractions were pooled and concentrated to 1mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-eluted motor domains (wild-type + jordan) were bound to a strong anion exchanger (5/50 MonoQ GL; Cytiva) using a Purifier 10 chromatography system (GE Healthcare). The column was washed with MOPS (10) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared through a Millipore 0.22 micron Durapore centrifugal filter and applied to a Superdex-200™ (10/300 GL) column (GE Healthcare) which was pre-equilibrated and then run in gel filtration buffer (TBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 kDa molecular weight cutoff) and purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), which was pre-equilibrated with buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was concentrated with a 100-kDa cut-off centricon (Milipore) and further purified by Superose-6 increase 10/300 column (GE Healthcare) using a buffer containing 50 mM Imidazole pH 6.0 ...
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bioRxiv - Microbiology 2020Quote: ... Fractions corresponding to assembled nanoparticles (elution between 8.5-10.5 mL with a peak at 9 mL) were pooled and concentrated at 350 × g by spin filtration with Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare). 16055 SOSIP-I53-50NPs were then buffer exchanged into PBS supplemented with 250 mM sucrose using a Slide-A-Lyzer MINI dialysis device (20 kDa molecular weight cutoff ...
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bioRxiv - Microbiology 2022Quote: ... Eluted mAb92 was further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were concentrated to 500 µl by Amicon Ultra 15 concentrators with 30 kDa cutoff and applied to a Superdex 200 10/300 Increase gel filtration column (GE Healthcare). Individual fractions containing the NTDyn and NDD were pooled and concentrated at 10 mg/ml.
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bioRxiv - Molecular Biology 2022Quote: ... SEC-MALS of GsSir2/Ago and CcSir2/Ago complexes was performed at room temperature using Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer (20 mM Tris–HCl (pH 8.0 at 25 °C) ...