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Citations for GE Life Sciences :
2701 - 2750 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 and 2E10.E9 Fab-PvCSPvk247 complexes were purified via Superose 6 Increase 10/300 GL chromatography (GE Healthcare) and concentrated to 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: Full-length recombinant PvCSPvk210 or PvCSPvk247 was complexed with a molar excess of 2F2 or 2E10.E9 Fab and loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 or 2E10.E9 Fab-PvCSPvk247 complexes were purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) and diluted to 50 µg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 3D11 Fab-NPNDx2 and 1210 Fab-NANP5 peptide complexes were purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare) and diluted to 25 µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluate was concentrated to below 500 μL and subjected to size-exclusion chromatography on a Superdex 200 10/300 Increase Analytical (S200a) column (GE Healthcare) equilibrated with 10 mM HEPES (pH 7.7) ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... HspA1A-containing fractions were once again combined and loaded onto a Superdex™ 200 Increase 10/300 GL (GE Life Sciences) size exclusion column ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was passed through a HiLoad 10/300 Superdex 75 GL size exclusion column using an ÄKTA pure purification system (GE Healthcare). Peak fractions were pooled and concentrated using Amicon Ultra-4 centrifugal filter units (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples of TRIM21 CC 235 (100uL) were resolved on a Superdex S-200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min in 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times with TBS-T for 10 min each before incubation with Amersham ECL detection reagent (GE Healthcare) and chemiluminescent detection using the Li-COR Odyssey FC imaging system (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) each ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein can be further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) as described above.
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bioRxiv - Biophysics 2022Quote: ... The individual complexes were incubated over ice for 1 hour and purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE healthcare) pre-equilibrated in a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Cytiva #SH30243.02) supplemented with 10% fetal bovine serum (GE Life Sciences #SH30910.03), 2 mM L-Glutamine (GE Life Sciences #SH30852.01) ...
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing EftMHM4 or EftMPAO1 were pooled and the protein further purified by gel filtration chromatography on a Superdex200 10/300 column (GE Healthcare) equilibrated with gel filtration buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... were analyzed in aliquots of 50 μl each at a sample concentration of 2 mg/ml (in 20 mM Hepes pH 7.5, 150 mM NaCl) on a Superdex 200 10/300 increase column (GE Healthcare, Germany) at a column temperature of 35 °C and a flow rate of 0.7 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... no spectral changes were observed for WT protein anymore and the sample was applied to a size exclusion column (HiLoad 10/300 Superdex 75 pg column, GE Healthcare) and the dimeric fraction of about 50% of the total protein was pooled ...
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bioRxiv - Molecular Biology 2019Quote: ... Gel was processed for Southern blotting: DNA was transferred on nylon membrane by passive transfer in 10×SSC buffer (Amersham Hybond XL, GE Healthcare). Membranes were UV-cross-linked and hybridized overnight with 32P-labeled single-stranded DNA probes at 42 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biophysics 2020Quote: ... The protein samples were centrifuged at 12,000g for 10 min to remove any protein precipitation and then loaded into a gel filtration column (Superdex 200 Increase 10/300GL, GE Healthcare) coupled with an 18 angle light scattering detector (DAWN HELEOS II ...
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bioRxiv - Plant Biology 2019Quote: ... Purification tags were removed by incubation with 3C protease (10 μg/mg fusion protein) followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/60 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was loaded at concentrations ranging from 2 to 16 mg/ml on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... dynein constructs were mixed with 20 μM Halo Alexa488 dye and incubated on ice for 10 min and a PD MiniTrap G-25 column (GE Healthcare) equilibrated with dynein gel filtration buffer was used to remove excess dye afterwards.
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated with a Vivaspin 100-kDa MWC0 concentrator and the monomeric FPN fraction was collected after size-exclusion chromatography (SEC) over a Superdex S200 Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.50 ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant CLASP2 C-term (300 μg, injection volume of 100 μl) was analyzed on a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated in buffer C ...
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged for 10 min at 5000 g and the lysate was passed through a Source-15Q anion exchange 55 ml column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... it was purified on a nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... All affinity-purified proteins were size-exclusion-purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare, catalog 28990944) in HEPES buffered saline (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2019Quote: ... The labeling reaction was quenched after 1 hr with fresh DTT at 100 mM final concentration and loaded onto a HiPrep 26/10 desalting column (GE Healthcare). The final labeled proteins were concentrated to at least 80 µM and flash-frozen in Protein Buffer (50 mM Tris-HCl [pH 7.2] ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the desired protein were pooled and gel filtration was performed on Superdex 75 or 200 10/300 GL columns (GE Healthcare) mounted into an ÄKTA P100 chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein was further purified using size-exclusion chromatography with a Superdex 75 10/300 GL column (GE Life Sciences) equilibrated in Buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were further purified by size-exclusion chromatography (on either a Superdex 200 increase 10/300 GL or a HiLoad 16/600 Superdex 200pg column, both GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The HiTrap ButylFF column of 1 mL and the gel filtration column Superdex 200 10/300 GL were from GE Healthcare. MacroPrep HighS resin was from Biorad ...
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... His6-MBP-TEV-NRBF2MIT was concentrated with a 10 kDa MWCO Amicon concentrator and injected over a Superdex-200 column (GE Healthcare) equilibrated in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... CDK1/CycA was concentrated and the holocomplex separated from free CDK1 by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare). Peak fractions were analyzed by SDS-PAGE analysis and fractions where CDK1 and CycA co-eluted were pooled ...
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bioRxiv - Microbiology 2019Quote: ... The holo-UbiV or holo-UbiU/V complexes were then loaded onto a Superdex 75 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer C or A ...
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bioRxiv - Biochemistry 2019Quote: SEC-MALS was conducted using a Wyatt Dawn 8+ detector (Wyatt Technology) coupled to a Superdex 75 10/300 column (GE Healthcare) and a refractive index detector ...
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bioRxiv - Biochemistry 2019Quote: ... The pooled protein was further purified by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare Life Sciences). MPEG-1 fractions were monitored using SDS-PAGE ...
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bioRxiv - Biophysics 2019Quote: ... the flow through from this step was concentrated using a centrifugal concentrator and applied to a Superdex200 10/300 GL (GE Healthcare) size exclusion column equilibrated with a “Standard” buffer of 20 mM Tris-HCl ...
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bioRxiv - Immunology 2019Quote: ... the cell suspension diluted in 1 mL of complete L15 medium was carefully layered on top of 5 mL of Percoll gradient (L15/10 mM HEPES/20% Percoll (GE Healthcare) and centrifuged at 400 g for 9 min ...
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bioRxiv - Biophysics 2019Quote: ... the labeled protein (M1-Alexa488) was separated from the free dye using size-exclusion chromatography (PD-10, GE Healthcare, Munich, Germany). The labeled protein was eluted with diluted PBS (pH 7.4) ...