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Citations for GE Life Sciences :
2651 - 2700 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, GE healthcare) and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Biochemistry 2023Quote: ... The untagged protein was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare), eluted in buffer E (25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... His6-MBP fused FoxP3ΔN variants were purified using Ni-NTA affinity column and Superdex 200 Increase 10/300 (GE Healthcare) SEC column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl.) supplemented with 2 mM CaCl2 and loaded onto a Superose6 Increase 10/300 GL column (GE Healthcare) integrated on a high-performance liquid chromatography (HPLC ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: Affinity purified samples were further purified by size exclusion chromatography over a Superdex 200 10/300 GL column (GE Healthcare) with running buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Immunology 2023Quote: ... Cell suspensions were filtered through a 40-μm cell strainer into RPMI with 10% FBS (GE Healthcare Life Sciences SH30071.03) and incubated for 5 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 200 mM NaCl and 2 mM TCEP using a Superdex-200 10/300 GL size-exclusion column from GE Healthcare. All protein concentrations were diluted to 1.7 mg/mL ...
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bioRxiv - Physiology 2024Quote: ... RNA was subjected to 8M urea–10% polyacrylamide denaturing gel and autoradiography using Typhoon FLA 9500 Fluorescence Imager (GE Healthcare). Band intensities were quantified using NIH Image J.
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bioRxiv - Immunology 2024Quote: ... The complexes of SH-01AF594 with C3d and Fab 3E12 were obtained by concentrating ∼1 mg of either protein with 300µg of SH-01AF594 to ∼200µL before removing the excess protein via SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in HBS ...
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bioRxiv - Plant Biology 2024Quote: ... and loaded to a gel filtration column Superdex 200 Increase 10/300 GL operated with AKTA Pure 25L (GE Healthcare). Gel filtration was performed in the Size Exclusion Chromatography (SEC ...
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bioRxiv - Microbiology 2024Quote: ... The deacylated tRNA was ethanol precipitated and dissolved in monoQ buffer A (40 mM sodium phosphate buffer, pH 7.0) and separated on the 8-ml MonoQ column (10/100, GE Healthcare), using two-step linear gradient of buffer B (A with 1M NaCl) ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Bioengineering 2024Quote: ... Final purification by size exclusion chromatography in PBS (pH 7.4) on a Superdex 200 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample (0.5 mL final volume) was loaded onto a Superdex 200 increase 10/300 size exclusion column (GE Healthcare) at a flow rate of 0.4 mL/min and the absorbance at 280 nm was monitored ...
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bioRxiv - Biochemistry 2024Quote: ... 50-100 μL of the purified protein samples (concentration ranging 2-15mg/ml) were filtered (with 0.1 μm pore size) and loaded onto a Superdex200 10/300GL column (GE Healthcare) preequilibrated with SEC buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Anion-exchange chromatography (AEX) was performed using a Q Sepharose Fast Flow Column (HiPrep Q FF 16/10; GE Healthcare) pre-washed with three column volumes of AEX Buffer B (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... Derivatized SCCs were further purified by SEC over a Superdex 75 10/300 GL column (GE Healthcare Bio-Sciences AB). ANDS-labeled SCCs were resolved on BoltTM Bis-Tris 4-12% Gel (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... nanobody was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5 ml of base buffer was then applied to PD10 columns to elute the nanobody protein base buffer without imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... protein was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5ml of base buffer was then applied to PD10 columns to elute the RECQL5 protein in base buffer ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... The concentrated flowthrough was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL Increase (GE Healthcare) column using 20 mM tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Mixtures were incubated at room temperature for 20 minutes and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with assembly buffer ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Biophysics 2020Quote: The oligomerization states of A3H Hap I mutants were determined by loading 120 to 300 μg of the purified enzymes onto a Superdex 200 10/300 Increase (GE Healthcare). The running buffer contained 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... Kif14 constructs were finally separated from the MBP tag by size-exclusion chromatography on a superpose 6 10/300 (GE Healthcare) column equilibrated with BRB80 buffer (80 mM Pipes (pH 6.8) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: Full-length PbCSP was co-complexed with a molar excess of 3D11 Fab and loaded on a Superose 6 Increase 10/300 GL (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Microbiology 2020Quote: ... the released proteins were further purified on a Superdex 200 (for nsp12) or a Superdex 75 (for nsp7 and nsp8) Increase 10/300 GL column (GE Healthcare) using 25 mM HEPES–NaOH ...
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bioRxiv - Biochemistry 2021Quote: ... and concentrated by ultrafiltration (Amicon, 10 kDa cutoff,) before further purifying on a Superdex S75 16/60 gel filtration column (GE Healthcare) in 25 mM NaH2PO4/Na2HPO4 pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to nitrocellulose (GE Healthcare. For preparation of cell wall extracts for mass spec ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration chromatography was performed using an AKTA FPLC System with a Superose 6 Increase 10/300 GL column (GE Healthcare) in 50 mM NaPO4 pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 10 mM maltose solution in the lysis buffer and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) before being subjected to additional purification by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... experiments coupled to multi-angle laser light scattering (MALS) and refractomestry (RI) were performed on a Superdex S200 10/300 GL increase (GE Healthcare) for NyxA and a Superdex S75 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... or Protein A resin (Pierce) before being subjected to additional purification by size-exclusion chromatography using either a Superose 6 10/300 column (GE Healthcare) or a Superdex 200 10/300 Increase column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated TANV16L was subjected to size-exclusion chromatography using a Superdex S200 increase 10/300 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... heated to 95°C for 5min and separated on a 10% SDS-PAGE gel before transferring onto a nitrocellulose membrane (Amersham Hybond ECL, GE Healthcare) using BioRad transfer apparatus ...
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bioRxiv - Microbiology 2019Quote: ... A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare) using 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2019Quote: We first performed buffer exchange of the purified BamAB-RcsF complex using a PD-10 desalting column (GE Healthcare Life Sciences). The complex was eluted with 20 mM HEPES [pH 7.5] ...
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bioRxiv - Molecular Biology 2021Quote: The AR9 nvRNAP core sample was polished and buffer-exchanged using size exclusion chromatography on a Superdex 200 10/300 (GE Healthcare) column equilibrated with buffer D containing 100 mM NaCl ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Molecular Biology 2022Quote: Hela and Hela-RNAseH1 cells downregulated for the indicated genes were grown in the presence of 10 μM bromodeoxyuridine (BrdU, GE Healthcare) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Plasma was separated by centrifugation at 1100 rpm for 10 minutes and PBMC was isolated from the buffy coat by Ficoll-Paque PLUS (GE Healthcare) and cryopreserved in fetal calf serum + 10% DMSO.
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bioRxiv - Biophysics 2022Quote: ... The protein of interest was separated by affinity chromatography using resin attached with Ni-NTA and being subjected to additional purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 and 200 mM NaCl.