Labshake search
Citations for GE Life Sciences :
2951 - 3000 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The his-tagged protein was eluted 4 CV of elution buffer (lysis buffer supplemented with 250 mM imidazole) and further purified by size exclusion chromatography in a Superdex 200 10/30 (GE Healthcare) equilibrated with 20 mM Tris pH 7.4 ...
-
bioRxiv - Microbiology 2022Quote: ... concentrated using a 30 kDa molecular weight cut-off (MWCO) Ultra Centrifugal tube (Amicon) and loaded to a Superose 6-Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES/NaOH pH 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were lysed and His-ALOD4 and His-OlyA were isolated by nickel purification followed by size exclusion chromatography (HisTrap-HP Ni column, Tricorn 10/300 Superdex 200 gel filtration column; FPLC AKTA, GE Healthcare). Protein-rich fractions were pooled and concentration was measured using a NanoDrop instrument.
-
bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was also evaluated via size exclusion chromatography by loading 400 µl of the Hitrap SP HP purified fraction onto Superdex 200 increase 10/300 GL column (GE Healthcare), and a monodisperse peak was obtained at 11.8 ml elution fraction.
-
bioRxiv - Biochemistry 2022Quote: ... Anion exchange chromatography was performed on an ÄKTA Pure system equipped with a 10/100 Tricorn column (Cytiva) packed with Source 15Q ion exchange resin (Amersham Biosciences, GE Healthcare) equilibrated in AEX buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
-
bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
-
bioRxiv - Biochemistry 2022Quote: ... 3C protease was removed by Ni-chelated sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
-
Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... 50-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 75 10/300 GL increase size-exclusion chromatography column (GE Healthcare) in 10 mM HEPES ...
-
bioRxiv - Biophysics 2022Quote: ... and purified from any other oligomeric species and salts using a Superdex 75 10/300 GL column (GE Healthcare, Chicago, IL) at a flow rate of 0.5 ml/min in 20 mM sodium phosphate buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... The fluorophores and other small molecules were removed from the proteins by passing the reaction mixture through a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with the Protein Buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... the TNC buffer in which proteins were stored was exchanged using Sephadex G-25M PD-10 desalting gravity columns (GE Healthcare) into 50 mM N-Cyclohexyl-2-aminoethanesulfonic acid (CHES ...
-
bioRxiv - Biophysics 2019Quote: ... The eluted protein fraction was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) in the buffer of 20 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biophysics 2019Quote: ... aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare). aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA ...
-
bioRxiv - Biophysics 2019Quote: ... Before experiments 1 mL of aSyn was further purified using a Superdex 75 pg 10/300 GL size exclusion chromatography (SEC) column (GE Healthcare) to obtain monomeric protein ...
-
bioRxiv - Cell Biology 2019Quote: ... PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with gel filtration buffer (20 mM PIPES pH 6.8 ...
-
bioRxiv - Biochemistry 2019Quote: ... the peak size-exclusion chromatography F86E RAD51 elution fraction was serially diluted (5, 1:1 serial dilutions) and loaded onto a Superdex200 10/300 GL size exclusion column (GE Healthcare) equilibrated with MALS Buffer (150 mM KCl ...
-
bioRxiv - Biochemistry 2019Quote: ... ~300 μL of 5 μM complex were mixed at a 1:1 molar ratio and incubated for 30 minutes at 4° before injection onto a Superdex200 10/300 GL analytical scale column (GE Healthcare). For CRY:PER2 CBD complexes used for BLI or CRY1:PER2 CBD:CLOCK:BMAL1 bHLH-PAS:Ebox DNA for cryo-EM ...
-
bioRxiv - Molecular Biology 2019Quote: ... A 10 μl aliquot of each reaction mixture was loaded on polyethyleneimine (PEI) cellulose thin-layer chromatography (TLC) plates (GE Healthcare) and separated by chromatography in 1.5 M potassium phosphate at pH 3.4 ...
-
bioRxiv - Plant Biology 2019Quote: ... and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns, GE Healthcare).
-
bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...
-
bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
-
bioRxiv - Biophysics 2019Quote: ... The beads were removed and the solution was further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biophysics 2019Quote: ... The DTT in the buffer for cysteine mutant of K48-diUb was removed by desalting (HiPrep 26/10 Desalting column, GE Healthcare), and the protein was reacted with the pre-mixed excess Alexa488 and Cy5 dyes ...
-
bioRxiv - Biochemistry 2019Quote: ... The final size-exclusion chromatography after affinity purification and tag removal was performed using Superdex75 16/60 HiLoad and Superdex75 increase 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.1 ml of this solution was subjected to SEC at 293 K on a Superdex 200 10/300 column (GE Healthcare) preequilibrated in 10 mM MES pH 6.0 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The eluted proteins were concentrated using Ultra-15 Centrifugal Filter Units (Amicon) and further purified by using a SuperdexTM 75 Increase 10/300 GL (GE Healthcare) size exclusion column in Tris Buffered Saline (TBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 µL of a 10 mg/mL mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare) were added to each sample ...
-
bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
-
bioRxiv - Cell Biology 2020Quote: ... MFG-E8-eGFP was further purified by gel filtration on an Äkta prime system with a Superdex 200 Increase 10/300 GL column (GE Healthcare).
-
bioRxiv - Cell Biology 2019Quote: ... 10 mM DTT and the flow-through (containing PFN1) was collected and then applied to a Superdex 75 (10/300) gel filtration column (GE Healthcare) equilibrated in 50 mM Tris (pH 8.0) ...
-
bioRxiv - Biochemistry 2019Quote: ... spun down 15 min at 15000 rpm in a bench-top centrifuge (4°C) and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare), previously equilibrated in gel filtration buffer (30 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2019Quote: ... The proteins were further purified by size exclusion chromatography (SEC) on a Superdex 75 Increase 10/300 GL column (GE Healthcare) for E2 cores and on a Superose 6 10/300 GL column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2019Quote: ... All samples were run in a 10% polyacrylamide native gel and then imaged using a Typhoon FLA9500 gel scanner (GE Healthcare).
-
bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
-
bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
-
bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluted protein was then exchanged into lysis buffer supplemented with 0.15% w/v OGNG / 0.015% w/v CHS by passing over a pre-equilibrated Sephadex PD-10 desalting column (GE Life Sciences). TEV protease was added to the desalted protein at a weight ratio of 1:10 for overnight tag cleavage ...
-
bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to remove remaining fluorophores and aggregates ...
-
bioRxiv - Biophysics 2020Quote: ... Fractions containing plexin B2 were pooled and subjected to size exclusion chromatography with a Superdex 200 GL 10/30 column (GE Healthcare) equilibrated with Buffer C containing 20 mM Tris (pH 8.0) ...
-
bioRxiv - Biophysics 2020Quote: ... the expression and purification was performed as outlined by Svetlov and Artsimovitch 45 with an additional size exclusion chromatography step performed using a Superose6 10/300 GL column (GE Healthcare) pre-equilibrated with PBS Buffer at 4°C instead of an ion exchange chromatography step ...
-
bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
-
bioRxiv - Plant Biology 2021Quote: ... The target proteins flowed through from the column were collected and further purified by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) with lysis buffer containing 5 mM DTT (Dithiothreitol) ...
-
bioRxiv - Neuroscience 2020Quote: SEC-MALS experiments of 100 μM WT and all mutants were carried out by using a Superdex 200 10/300 column (GE Healthcare) in SEC buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
-
bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...