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Citations for GE Life Sciences :
2801 - 2850 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to less than 1 mL and was injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biophysics 2021Quote: ... 500 mM imidazole) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL column, GE Healthcare). Protein concentration is determined using the protein extinction coefficient and corrected for direct absorption of the fluorophores at 280 nm ...
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bioRxiv - Biophysics 2021Quote: ... the protein was finally eluted by 230 mM imidazole and further purified through size exclusion chromatography (SEC, Superose 6, 10/300, GE Healthcare) with Lysis Buffer containing 0.001% LMNG ...
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bioRxiv - Plant Biology 2020Quote: ... The 200 μL of filtered solubilized fraction was used for gel filtration chromatography using the ÄKTAmicro chromatography system with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM HEPES-NaOH (pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated using an Amicon Ultra filter with 10 kDa cutoff and loaded on a HiLoad 16/600 Superdex 200 pg (GE Healthcare) size exclusion chromatography column equilibrated with SEC-buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... the clarified supernatant was purified on strep-tactin resin (IBA) followed by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare) equilibrated with 10 mM Tris-HCl pH 8.0 and 200 mM NaCl as the running buffer (SEC buffer) ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... Sub16pro was separated from the cleaved tag by loading the protein on a Ni-NTA column and finally purified by size-exclusion chromatography over a Superdex 75 10/300 GL gel filtration column (GE Healthcare) and stored in buffer P (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Biochemistry 2021Quote: ... iMAC samples were thawed on ice and loaded into a purification column packed with 10 mL of Sephacryl S-100 (GE Healthcare) equilibrated with 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... NSs::6xHIS was concentrated using a 10 kDa MWCO spin concentrator (Amicon) and further purified by size exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Elution Buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Uncleaved protein was removed by incubation with TALON Superflow resin before concentrating for loading onto a Superdex 75 Increase 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Complexes were purified by SEC on a Superdex 200 Increase GL 50/150 or a Superdex 200 Increase 10/300 GL column (GE Healthcare) and fractions containing CD4m-BG505-17b complexes were concentrated to 1.4-1.5mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol and 1 mM DTT prior to ion exchange chromatography using a Fast Flow SP column (GE Healthcare). Elution from the SPFF column was performed with a 100 mM to 1M gradient over 20 column volumes ...
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bioRxiv - Bioengineering 2021Quote: ... The biotinylation mixture was incubated at room temperature for 1 h and then loaded onto a HiPrep 26/10 desalting column (GE Healthcare) pre-equilibrated with a storage buffer (1×PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... the eluants were further applied to size exclusion chromatography with the Superdex 75 or Superdex 200 column with ÄKTA Purifier 10 FPLC system (GE Healthcare). The E ...
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bioRxiv - Plant Biology 2020Quote: ... PAIPP2-3FLAG and CPL2-4MYC with IP buffer and then loaded on to a Superdex 200 10/300GL column (GE Healthcare). The eluted fractions were collected in 96-well plates and the target proteins were detected by standard western blotting.
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bioRxiv - Microbiology 2020Quote: ... Biotech AB/Sweden) prior to protein separation on a S200 Superdex 10/300GL column on an Äkta Purifier (both GE Healthcare). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL; GE Healthcare) equilibrated with 50 mM Bis-Tris pH 6.5 ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:10,000 in TBS-T with 3% BSA and finally washed 3 x in TBS-T for 10 min before adding ECL substrate for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... Cleaved proteins were subsequently purified from the protease and affinity tag by a second Ni2+-affinity column or by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare). Proteins were concentrated to 3–10 mg/ml and either used immediately or aliquoted and quickly frozen at –80°C ...
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bioRxiv - Microbiology 2020Quote: ... Excess DNA was removed from conjugated proteins using size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) in a 25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Plant Biology 2021Quote: ... using an AKTA Pure 25 system equipped with HisTrap HP 1 mL column and submitted to gel filtration using a Superdex 200 increase 10/300 GL column (GE healthcare). Purified recombinant proteins were conserved at −80°C in 1x PBS solution containing 10% glycerol.
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bioRxiv - Microbiology 2020Quote: ... The bacteria lysate containing GST-Avr1d was incubated with 10 μl glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare) for 3 hours and purified by washing with 1×PBS for 5 times ...
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bioRxiv - Microbiology 2020Quote: ... The methylated VSG13 N-terminal domain was purified by gel filtration on a Superdex 200 Increase 10/300 GL (GE Healthcare) in 10 mM Tris.Cl ...
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bioRxiv - Microbiology 2021Quote: PdeL samples (100 µl) varying from 11 to 371 µM were loaded onto a Superdex 200 (10/300) column (GE Healthcare) at constant flow (0.5 ml/min ...
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bioRxiv - Microbiology 2021Quote: ... the eluted proteins were concentrated to ∼200 µL and purified by size-exclusion chromatography on a Superose 6 10/300 GL column (GE Healthcare) in 10 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved protein was finally purified by size-exclusion chromatography (SEC) on a Superose 6 10/300 GL column (GE Healthcare) in 25 mM Tris (pH 8) ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... For X-ray crystallography the protein was further purified using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare) over a HiPrep 16/60 Sephacryl S-100 high-resolution gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The chymotrypsin-treated protein was further purified by size exclusion chromatography using a Superdex 200 HR 10/30 column with an AKTA FPLC system (GE Healthcare) in buffer C (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... were used depending on the enzyme availability) and were further purified by gel-filtration chromatography on Superdex 200 10/300 column (GE Healthcare) in Buffer A (20 mM HEPES-KOH pH7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 66 kDa) were used to generate a molecular weight standard plot using a Superdex 200 10/300 GL column (GE Healthcare). A 3.2 mg/ml sample of His-PodJ(1-635 ...
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bioRxiv - Cancer Biology 2019Quote: ... The eluted proteins were further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The sample (100 µL of 1 mg/mL UL21-H6) was injected onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in 20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... HSF2BP and ARM proteins were loaded in the presence and absence of the BRCA2 fragment F15X on a Superdex 200 10/300 GL (GE Healthcare) using a HPLC Shimadzu system coupled to MALS/QELS/UV/RI (Wyatt Technology) ...
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bioRxiv - Molecular Biology 2020Quote: ... divided into aliquots of 10 mL and each aliquot was added on top of a 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μl of each reaction mixture was loaded on polyethyleneimine (PEI) cellulose thin-layer chromatography (TLC) plates (purchased from GE Healthcare) and separated by chromatography in 1.5M potassium phosphate at pH 3.4 ...
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bioRxiv - Microbiology 2020Quote: ... Purified protein obtained using glutathione affinity chromatography was then subjected to size-exclusion chromatography using a Superdex S75 10/300 or 16/600 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...