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Citations for GE Life Sciences :
2401 - 2450 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the protein were concentrated and gel filtered on a Superdex 75 increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer.
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bioRxiv - Cell Biology 2020Quote: ... The fractions were thawed (or never frozen) and concentrated for size exclusion chromatography on a Superdex200 10/300 (GE Healthcare) preequilibrated in 10mM Tris pH 8 ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... the protein fraction in the supernatant was resolved on a 10 % SDS-PAGE gel and transferred to a 0.2-μm nitrocellulose membrane (Amersham, GE Healthcare). The membrane was blocked with 5 % (w/v ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The complex was incubated for 10 min at 22°C before size exclusion chromatography using a Superdex 200 Increase 3.2/300 column (GE Healthcare) at 22°C ...
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bioRxiv - Immunology 2021Quote: ... and further purified by size exclusion chromatography on a Superdex 75™ 10/300 GL column (GE Healthcare Life Sciences) in standard assay buffer (10 mM MES ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was then centrifuged at 18,800 × g for 10 min and the resulting supernatant was applied to His Spin Trap (GE Healthcare). After centrifugation (100 × g ...
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bioRxiv - Microbiology 2019Quote: ... Purified protein was immediately exchanged into TEV cleavage buffer (50 mM Tris-HCl pH 8.0, 10% glycerol) using a 50 ml desalting column (GE Healthcare). For His-tag cleavage ...
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Biochemistry 2022Quote: ... The concentrate was separated with a flow rate of 0.5 ml/min on a Superdex 200 10/300 GL column (GE Healthcare, Little
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bioRxiv - Molecular Biology 2021Quote: Released protein was further concentrated to 0.5mL and then loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins (500 μl) at a concentration of 1.5 mg/ml were loaded on Superdex 75 10/300 GL column (GE Healthcare) and eluted with HEPES buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... The cleared supernatant was purified through Histrap HP and gel-filtered on a Superdex 200 10/300 columns (GE Healthcare). The LacI-GFP-MAD1 fragments were generated by PCR amplification of MAD1 and insertion into the LacI-GFP vector [54] ...
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bioRxiv - Biochemistry 2019Quote: ... MK2 was subsequently removed from the reaction mix by another round of SEC (Superdex 75, 10/300 GL, GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... concentrated with Amicon Millipore 15 ml 100,000 MWCO centrifugal concentrator and applied to Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated in storage buffer (400M KCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Germany) reconstituted in water was run on a Superdex 200 increase 10/300 column /Äkta Pure system (GE healthcare, Germany) equilibrated with 20mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was concentrated on an Amicon Ultra Centrifugal Filter Unit (10 kDa cut-off) and injected at 0.5 ml/min on a Hi-Load 16/60 Superdex column (GE healthcare), equilibrated with 20 mM Tris-HCl buffer ...
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bioRxiv - Microbiology 2019Quote: ... Intact complexes were separated from aggregate by size-exclusion chromatography in TBS containing 0.01% (w/v) LMNG (S200 10/300 increase or Superose 6 increase, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... STAR635-conjugated mSav* was purified by gel filtration using Superdex 75 (Superdex 75, 10/300 GL, GE Healthcare Life Sciences). Fractions containing monomeric mSav*-STAR635 were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 ml of fractions containing the highest protein content were pooled and the imidazole was removed by running the 2.5 ml protein suspension on PD-10 size-exclusion columns (GE Healthcare) followed by cold 3.5 ml of Buffer A to elute proteins ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... Refolded protein was concentrated (Vivaspin or Amicon 10 kDa) and further purified on Superdex S75 or S200 columns (GE Healthcare) in SEC buffer as indicated in Table S3 ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting filtrates were analyzed by size-exclusion column chromatography using Superose 6 Increase 10/150 GL (GE healthcare). Peak values at the retention time for the complex were plotted as a function of incubation temperature ...
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bioRxiv - Biochemistry 2019Quote: ... hBrr2T1 variants were combined with a 1.5 molar excess of hJab1 variants and loaded on a Superdex 200 10/300 global increase column (GE Healthcare) in 20 mM TRIS-HCl ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... and adjusted to pH 8.0 in 10 mM Tris before purification in an AKTA Avant system by Steptrap-HP chromatography (GE Healthcare). Final purification was achieved by gel filtration using a Superdex 75 HiLoad 16/60 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare), equilibrated with Tris-HCl buffer (50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The obtained material was concentrated to reach a final volume ≤0.5 mL and further purified by SEC on a Superdex 200 10/300 Increase column (GE Healthcare), equilibrated with buffer GF (200 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... Samples were separated in a 10% acrylamide (Roth, Karlsruhe, Germany) gel and blotted onto nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in a wet system ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Biophysics 2021Quote: ... The Cy5 labelled MukB was purified by size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare). Peak fractions were pooled and concentrated by ultrafiltration.
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bioRxiv - Plant Biology 2020Quote: Purified His-tagged proteins EH1.1 (1mg/ml) or EH1.2 (2mg/ml) were injected onto a Superdex 75 Increase 10/300 GL size exclusion column (GE Healthcare), equilibrated with 20mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was separated from free D3_Sof protein by size-exclusion chromatography using “Superdex 75 10/300 GL” column (GE Healthcare) at 0.4 ml/min flow rate using running buffer containing 25 mM Tris-HCl (pH 7.6 at 25 ° C) ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... 2 nmol of FLhArc were incubated with 1.5-fold molar excess Nbs and then applied to a Superdex 200 Increase 10/300 GL column (GE healthcare) equilibrated in PBS buffer.
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bioRxiv - Biochemistry 2020Quote: ... These two complexes were then mixed together with 1.5x molar excess of Nup84-Nup133 and run over a Sepharose 6 10/300 column (GE Healthcare). Fractions containing all Y complex components were pooled and concentrated.
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bioRxiv - Biochemistry 2020Quote: ... for 30 minutes with or without cofactor GMPPNP (30 μM) then applying 0.5 mL to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare). Eluted fractions (0.5 mL ...
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bioRxiv - Biochemistry 2020Quote: The native molecular mass of BdiUSPPase and BdiGolSase1 were determined by using a Superdex 200 10/300 column (GE Healthcare), equilibrated with Buffer G ...
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bioRxiv - Biochemistry 2020Quote: ... Inc) and subsequently subjected to further purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) with the buffer of 75mM Tris (pH7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was further purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2020Quote: ... Size-exclusion chromatography was performed on an ÄKTA FPLC system using a Superdex 200 increase 10/300GL column (GE Healthcare). Protein binding affinity was performed by Surface Plasmon Resonance using a BIAcore X100 instrument using a CM5 chip coated with recombinant fibronectin 11A12 domain ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
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bioRxiv - Biochemistry 2020Quote: ... After the reaction, the sample was centrifuged briefly (10 min, 16,000g, 4°C) and loaded onto a Superdex 200 column (GE Healthcare) to remove Sfp and unreacted dsOligo-CoA ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biochemistry 2020Quote: ... 10% glycerol and 0.1% triton X100 by using a desalting column PD Spin Trap G25 (GE healthcare: 28-9180-04). This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001 ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted for 7 s at force 10 using single-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Cell Biology 2021Quote: ... The ERMS cells were maintained in RPMI1640 (Nacalai, Osaka, Japan) with 10% fetal bovine serum (HyClone; GE Healthcare, UT, USA), 100 units/ml penicillin ...
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bioRxiv - Biophysics 2021Quote: ... The complex protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...