Labshake search
Citations for GE Life Sciences :
1951 - 2000 of 2000 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... by injecting the sample onto a 24 x 1 cm size exclusion column containing Sepharose 4B Fast Flow resin (pore size 84 nm) set-up in an AKTA Start System (GE Healthcare Life Science). EVs were eluted in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Dot blots were developed using chemiluminescence (ECL BPS-Bioscience®) and visualized with a LAS Fujifilm 4000 system (GE Healthcare Life Sciences®).
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bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection and quantification were performed using HRP-coupled secondary antibodies and and an Image Quant LAS4000 chemiluminescence-based detection system (enhanced chemiluminescence) (GE Healthcare Life Science).
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bioRxiv - Microbiology 2024Quote: ... The His-tag S protein was purified by gel filtration using the Akta explorer 100 system with a high-load Superdex pg 200 16/600 column (GE Life Sciences, USA). Main peak fraction was collected ...
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bioRxiv - Cell Biology 2024Quote: ... This was injected into a size-exclusion column (column size 24 cm x 1 cm) containing Sepharose 4B (84 nm pore size) using an AKTA start system (GE Healthcare Life Science) and eluted with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before[30] ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl were applied to an Äkta pure chromatography system equipped with a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) and run at 6 °C ...
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bioRxiv - Microbiology 2019Quote: ... Imaging was performed using either a Nikon Ti Inverted microscope fitted with a Lumencor Spectra X light engine or a v4 DeltaVision OMX 3D-SIM system (Applied Precision, GE Healthcare, Issaquah, USA).
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bioRxiv - Pathology 2019Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Cell Biology 2020Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL™ Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Biophysics 2020Quote: ... Purification was performed using size exclusion chromatography (SEC) on an Äkta purifier (FPLC) system with a Superdex200 10/300 column from (GE Healthcare Life Science; S200). We found that reconstitution ratios of 1:33 for ΔH4H5:DMPC and 1:50 for ΔH5:DMPC resulted in a single and relatively narrow symmetric peak ...
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Size-exclusion chromatography was performed on a 24 ml Superdex-75 gel filtration column on an ÄKTA Purifier System (GE Healthcare, Chicago, Illinois, USA) using the same buffer as during sedimentation velocity measurement ...
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bioRxiv - Biophysics 2022Quote: ... The sample was then injected in an Äkta Purifier System equipped with a Superdex Peptide 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with ultrapure water ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescence recovery after photobleaching (FRAP) analysis of GFP-AURKA-expressing cells was performed using a DeltaVision system and Photokinetics data analysis software (GE Healthcare, Pittsburgh, PA, USA). A centrosomal area of approximately 3 μm diameter was bleached using a 488 nm Quantifiable Laser Module while collecting time-lapse images at 1 s intervals.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified using a NiNTA purification system (HisTrap™ excel 1 mL) and size-exclusion chromatography using HiPrep™ 26/60 Sephacryl™ S-100 HR (GE Healthcare) in 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pathology 2020Quote: ... Equal volumes of plasma were pooled from three mice and lipoproteins were fractionated by fast protein liquid chromatography (ÄKTA pure FPLC system, GE Healthcare, Pittsburgh, PA, USA) and triglyceride and cholesterol in fractions quantified with reagent kits (Wako Diagnostics ...
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bioRxiv - Physiology 2020Quote: ... Examinations were carried out with a linear array i13L probe (10-14 MHz) using a Vivid 7 Dimension ultrasound system (GE Healthcare Ultrasound, Horten, Norway). The operator was blinded to the genotype of the mice ...
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bioRxiv - Physiology 2020Quote: ... was measured via laser Doppler flowmetry (Moor Instruments, Wilmington, DE, USA) and arterial blood pressure (Finapres Medical Systems, Enschede, Netherlands; GE Healthcare, Boston, MA, USA). Additionally ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Microbiology 2020Quote: ... Rv1339 protein was visualized with a FujiFilm LAS-3000 Image Reader and the Amersham™ ECL™ Western Blotting Analysis system (GE Healthcare, UK).
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bioRxiv - Molecular Biology 2021Quote: ... Samples for the DLS system were prepared in the droplet buffer and filtered through 0.02 mm Whatman Anotop sterile syringe filters (GE Healthcare Life Sciences, Pittsburgh, PA) into a 96-well plate (Wyatt Technology ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were imaged for GFP and mCherry 24 h after initial plating (t=0) and at the indicated timepoints using a 4X objective InCell Analyzer system (GE Healthcare Life Sciences, Marlborough). Segmentation and counting of GFP- and mCherry-positive cells were performed using an Acapella script (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a Superdex® 200 Increase 10/300 GL on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with PBS (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The denatured DNA was then blotted onto a Hybond LFP membrane using a Minifold I dot blot system (both from GE Healthcare, Piscataway, NJ, USA) followed by a wash with 400 µL TE buffer per well ...
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bioRxiv - Microbiology 2023Quote: ... After centrifugation the supernatant was applied to a HisTrap HP column connected to an Äkta Prime Plus system (both GE Healthcare, Chicago, Illinois, USA), and eluted after washing using 500 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... hst5:eGFP and hst6:eGFP cells were visualized using a DeltaVision microscopy system comprising an Olympus IX71 microscope and CoolSnap HQ camera (Applied Precision, Issaquah WA, USA). To visualize mitochondria ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Microbiology 2023Quote: ... aMCFCA was then purified from the supernatant using the AKTA protein purification system through a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02) with loading buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Physiology 2023Quote: ... Immunoreactive bands were detected using a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after the membranes were incubated with ECL Prime Western Blotting Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analysis of the immunoreactive bands was performed using the FluorChem FC3 Analysis Software.
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before(60) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).
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bioRxiv - Microbiology 2019Quote: ... Imaging was performed using either a Nikon Ti Inverted microscope fitted with a Lumencor Spectra X light engine or a v4 DeltaVision OMX 3D-SIM system (Applied Precision, GE Healthcare, Issaquah, USA).
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bioRxiv - Biophysics 2020Quote: ... thrombin cleavage of the His6-tag overnight and final ion exchange chromatography with a MonoQ or MonoS column (Äkta purifying system, GE Healthcare, Little Chalfont, United Kingdom). Synaptobrevin 2 49-96 S79C was labeled with Atto488 maleimide ...
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude). The column was equilibrated in 500 mM sodium chloride ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Bioengineering 2021Quote: ... A second purification step was performed for IDO-Gal3 using size exclusion chromatography in an ÄKTA Pure Protein Purification System (GE Life Sciences, Marlborough, MA, USA). Molecular weight and purity were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Bioengineering 2022Quote: VLPs were purified on an Akta Pure FPLC system via SEC with a HiPrep Sephacryl 16/60 S-500 HR (GE Healthcare Life Sciences, Cat# 28935607) column ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Genetics 2021Quote: ... Twenty-five ng were labelled with 50 µCi [α- 32P]dCTP (SRP-305; Hartmann Analytic; Braunschweig; Germany) with the Amersham Rediprime II Random Prime Labeling System (RPN1633; GE Healthcare, Chalfont St Giles, UK) and purified on Illustra G-50 ProbeQuant micro columns (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting using the ECL Select Western Blotting Detection System (GE Healthcare Japan Co., Ltd., Tokyo, Japan) and a LAS-3000 imaging system (GE Healthcare) ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Genomics 2019Quote: ... cell lysis and whole-genome amplification steps were carried out in Fluidigm C1 system using illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences cat: 25-6600-30). DNA concentrations of amplified single-cell genomes were determined using either a Qubit dsDNA HS Assay kit (Invitrogen cat:Q32854 ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...