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Citations for GE Life Sciences :
1701 - 1750 of 2000 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... then milliQ water before mounting as above but using Vectashield mounting medium (no DAPI) and imaged within a week using the DeltaVision OMX V3 Blaze system (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: Proteins were concentrated with appropriate centrifugal filtration devices (Amicon) and further purified by SEC at 4°C using an ÄKTAFPLC chromatography system (GE Healthcare). For crystallographic studies ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was eluted by using purification buffer including 200 mM imidazole and further purified by gel chromatography on an ÄKTA pure system equipped with a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) running with purification buffer ...
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bioRxiv - Neuroscience 2019Quote: ... peptides were further fractionated prior to LC-MS by reversed-phase HPLC using an Ettan ÄKTA micro HPLC system (GE Healthcare), as described elsewhere98.
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bioRxiv - Physiology 2019Quote: We purified individual isoHbs by means of anion-exchange fast-protein liquid chromatography (FPLC) using an Äkta Pure system (GE Healthcare). Stripped hemolysate was loaded on the prepacked HiTrap Q-HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and Waters 410 differential refractometer (Waters) in line with an ÄKTA pure chromatography system fitted with a Superdex 200 Increase column (both GE Healthcare). Data was analysed using ASTRA 5.3.4 software (Wyatt Technology).
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bioRxiv - Genetics 2020Quote: ... the microscopic images of the stained cells were obtained by a high-throughput automated image capturing system IN Cell Analyzer 2200 (GE Healthcare). The images were analyzed by a computer software CalMorph ...
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bioRxiv - Biochemistry 2020Quote: ... the V2R was purified by size exclusion chromatography (SEC) using a Superdex 200 (10/300 column) connected to an AKTA purifier system (GE Healthcare). Fractions corresponding to the pure monomeric receptor were pooled (~ 2 ml ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were centrifuged at 18000 rpm (F21-8×50y rotor) for 45 min and supernatants were purified by Ni2+-affinity chromatography (FPLC system ÄKTA Purifier, GE Healthcare) eluting the protein in 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: Elution peaks corresponding to a molar mass of a dodecameric or dimeric protein of PrgL32-208 were further analysed by SEC-MALS with the use of an ÄKTApure system (GE Healthcare) coupled to a miniDAWN TREOS II detector and an OptiLab T-rEX online refractive index detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2020Quote: 100-500 µl samples (lipid-bound and lipid-free ApoE3 and ApoE380-255) were applied to an AKTA protein purification system (GE) equipped with a Superdex 200 10/300 (GE healthcare) column and a 500 µl sample loop and run at 0.5 ml/min at 5°C ...
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bioRxiv - Biophysics 2021Quote: ... 500 mM imidazole) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL column, GE Healthcare). Protein concentration is determined using the protein extinction coefficient and corrected for direct absorption of the fluorophores at 280 nm ...
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bioRxiv - Plant Biology 2020Quote: ... The 200 μL of filtered solubilized fraction was used for gel filtration chromatography using the ÄKTAmicro chromatography system with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM HEPES-NaOH (pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... 4°C for 30 minutes and loaded onto a 5 ml HighTrap MBP column (GE Healtcare) using an Äkta-Pure system (GE Healthcare). The column was washed with lysis buffer and protein eluted with 10 column volumes MBP-elution buffer (100 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 0.1% Tween 20), and analyzed using enhanced chemiluminescence (WesternBrightTM ECL, Advasta) by ImageQuant™ LAS 4000 system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: Cells visualized on an Olympus IX-70 inverted microscope using 40x/1.35 oil objectives supported by a DeltaVision deconvolution system (Applied Precision LLC) with SoftWorx image acquisition and analysis software.10 focal planes at 0.2 μm per z-stack were taken using a Roper CoolSNAP HQ CCD camera ...
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bioRxiv - Biochemistry 2021Quote: ... Purification of enzyme having N-terminal hexahistidine tag was done by Ni-NTA affinity chromatography using an Akta pure FPLC system (GE Healthcare). Equilibration buffer was 50 mM tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with loading buffer containing 400 mM imidazole and then purified by gel filtration with a Superdex 200 column on the ÄKTAprime plus system (GE Healthcare) in binding buffer (20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were cut overnight at 4°C with 6X-His tagged ULP Sumo protease followed by gel filtration chromatography using a Superdex 200 gel filtration column attached to an AKTA Pure FPLC chromatography system (GE Healthcare). Peak fractions were collected and concentrated ...
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bioRxiv - Plant Biology 2021Quote: ... the eluants were further applied to size exclusion chromatography with the Superdex 75 or Superdex 200 column with ÄKTA Purifier 10 FPLC system (GE Healthcare). The E ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were mixed and subjected to fractionation by strong cation exchange (SCX) chromatography using the AKTA Purifier system (GE Healthcare). Fractions were desalted on C18 Cartridges (Empore™ SPE Cartridges C18 ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...
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bioRxiv - Molecular Biology 2021Quote: ... The chymotrypsin-treated protein was further purified by size exclusion chromatography using a Superdex 200 HR 10/30 column with an AKTA FPLC system (GE Healthcare) in buffer C (50 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... Immobilized Metal Affinity Chromatography (IMAC) was performed for protein purification using an AKTA Start Chromatography system (GE Healthcare Bio-Science, Sweden). The soluble fraction containing TNFα was diluted (1:1 ...
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bioRxiv - Microbiology 2020Quote: ... Purified protein obtained using glutathione affinity chromatography was then subjected to size-exclusion chromatography using a Superdex S75 10/300 or 16/600 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: The purification steps for both POLA1–POLA2–PRIM1–PRIM2 and p50–Ctc1–Stn1–Ten1 were performed at 4°C using an AKTA chromatography system with prepacked columns (GE Healthcare), following the same protocol ...
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bioRxiv - Biophysics 2021Quote: Size-exclusion chromatography was carried out using a Superdex 75 (HR 10/30) coupled to an Äkta purifier system (GE Healthcare), equilibrated with a 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: Recombinant N-terminal His6-tagged TRF1 was purified using a baculovirus/insect cell expression system and an AKTA Explorer FPLC (GE Healthcare) as described in a previous study (66) ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were subsequently purified via affinity chromatography using HisTrap or MBPtrap columns connected to an ÄKTA Explorer liquid chromatography system (GE Healthcare) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Plant Biology 2022Quote: ... Gel filtration chromatography was performed as described in (Iwai et al., 2015) using the ÄKTAmicro chromatography system with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Bioengineering 2022Quote: ... and Neq2X7 were purified by affinity chromatography using a 1ml HisTrap™HP on an ÄKTA Pure chromatography system (GE Healthcare). After washing of the column ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-tag was removed by a second affinity chromatography step with glutathione-agarose and the proteins in the flow-through were further purified by size exclusion chromatography using an Äkta Purifier System equipped with a HiLoad 26/600 Superdex 200 column (GE Healthcare). Protein-containing fractions were pooled and dialyzed against storage buffer (30 mM HEPES ...
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bioRxiv - Cancer Biology 2020Quote: ... Quality control of the purified products included SDS-PAGE and size exclusion chromatography using an Äkta Pure FPLC system (GE Healthcare) with a Superdex S200 10/300 increase column at a flow rate of 0.75 mL/min (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected using Western Lightning Plus-ECL (Perkin-Elmer, NEL104001EA) and images were acquired on an ImageQuant LAS4000 system (GE Healthcare). Densitometry analysis was carried out using ImageQuant TL software.
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bioRxiv - Immunology 2020Quote: ... Proteins were purified to homogeneity by protein A affinity chromatography and characterized by size exclusion chromatography on a Äkta Pure FPLC system (GE Healthcare) with a Superdex S200 10/300 increase column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Bands were visualised by enhanced chemiluminescence using ECL Prime Western Blotting detection reagents and using an ImageQuant LAS4000 system (GE Healthcare). ImageQuant TL 8.1 software (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was centrifuged for 16 hours at 40,000 g on a Beckman Coulter SW32 Ti rotor and fractionated using a Brandel fractionator apparatus and an AKTA Prime FPLC system (GE Healthcare). Fractions containing the 30S subunits were selected and pooled together based on the UV absorbance at A254 ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution fractions were loaded onto a SuperoseTM6 10/300 GL column previously equilibrated with buffer L using an ÄKTA Pure system (GE Healthcare). The purified His-SctV9-PR Peptidisc complex was subsequently analyzed by negative-stain electron microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing mainly His-SctV-C9 were further purified on a Superdex200 10/300 GL column previously equilibrated with buffer M using an ÄKTA Pure system (GE Healthcare). His-SctV-C9 complexes were subsequently analyzed by cryo-EM.
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bioRxiv - Biochemistry 2020Quote: ... and a two-step fast protein liquid chromatography (FPLC) purification protocol using an ÄKTA Protein Purification System (GE Healthcare Life Sciences) at 8 °C was then followed to obtain the recombinant protein ...
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bioRxiv - Immunology 2021Quote: The purification of S protein from cell broth was performed using on AKTA Pilot 600R system (GE Healthcare, Little Chalfont, UK). After harvesting ...
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bioRxiv - Bioengineering 2020Quote: ... were isolated from Trima Accel leukoreduction system (LRS, Memorial Blood Center) chambers using ammonium chloride-based red blood cell lysis followed by Ficoll-Paque gradient (GE Healthcare). PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The samples were then applied to IPG strips and separated in the first dimension with the IPGphor 3 system from GE Healthcare according to their isoelectric focusing (20 C ...
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bioRxiv - Immunology 2022Quote: The affinity between H2 and A33 was measured at room temperature using a Biacore T100 system with CM5 chips (GE Healthcare) according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Biochemistry 2022Quote: ... Anion exchange chromatography was performed on an ÄKTA Pure system equipped with a 10/100 Tricorn column (Cytiva) packed with Source 15Q ion exchange resin (Amersham Biosciences, GE Healthcare) equilibrated in AEX buffer ...