Labshake search
Citations for GE Life Sciences :
1901 - 1950 of 2000 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Immunoreactive proteins were detected in a ImageQuant LAS 4000 system with Amersham ECL Prime chemiluminescent reagent according to the manufacturer’s protocol (GE Healthcare Life Sciences). Primary antibodies used in this study were KLF4 (AF3158 ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein eluate was buffer exchanged into 1X PBS and then purified by size exclusion chromatography using a Superdex 200 Increase 10/300 GL Sizing column on the AKTA FPLC system (GE Life Sciences). The fractions of purified protein were analyzed by SDS-PAGE and binding was confirmed using ELISA with known antibodies.
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bioRxiv - Molecular Biology 2024Quote: SEC-MALS experiments were performed using and Ä KTA purifier system connected to a Superose 6 Increase 10/300 GL column (GE healthcare) at a flow rate of 0.4 ml/min in running buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the filtrate was applied to a HisTrap™ 5 ml Fast Flow (Cytiva) equilibrated in lysis buffer using an Äkta Purifier system (GE Healthcare). The column was washed with lysis buffer (100 mM Tris/HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were lysed by sonication and the fusion protein was purified using HisTrap HP histidine-tagged protein purification column of AKTA pure FPLC system (GE Healthcare, Pittsburgh, PA). The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
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bioRxiv - Cell Biology 2020Quote: ... were used first for an affinity-based batch purification process which was followed by size exclusion chromatography using an ÄKTApurifier system and a High Load 16/60 Superdex 200 SEC column (both GE Healthcare Life Sciences). Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM TCEP) using an AKTA pure chromatography system with a Superdex 200 10/300 GL column attached (GE Healthcare, GE17-5175-01). The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The clarified supernatant was applied to a Ni2+-charged IMAC column with chromatography carried out on an AKTATM purification system (GE Healthcare, Chicago, IL). The loaded column was washed with additional IMAC binding buffer and then elution carried out using an elution buffer (0.5 M imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Biophysics 2022Quote: ... After purification with Ni-NTA agarose the protein was diluted to 375mM NaCl and further purified on an ÄKTA purifier system (GE Healthcare Life Sciences) at 4 °C using 1ml HiTrap™ Heparin HP column (Cytiva) ...
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bioRxiv - Neuroscience 2019Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Biophysics 2020Quote: ... SHP-1 was further purified by size-exclusion chromatography on an AKTA fast protein liquid chromatography system equipped with a Superdex S200 10/300 GL column (both from GE Healthcare Life Sciences) equilibrated with 10 mM Hepes (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: ... gel filtration column with a flow rate of 1 ml/min using FPLC systems (AKTA pure 25 and AKTA FPLC, GE Healthcare Life Science). The 5 ml sample concentrated ...
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bioRxiv - Cell Biology 2021Quote: ... Kif7-DME502A and the Kif7-Kif27 chimeric constructs were expressed in SF9 insect cell line using the Bac-to-Bac® Baculovirus Expressions System (Thermo) with cells grown in HyClone CCM3 SFM (GE Life Sciences) and expressed from P3 virus for 72H at 27°C ...
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bioRxiv - Microbiology 2020Quote: ... This was performed using an purifier system connected to a Superose 6 Increase 10/300 GL column (both from GE healthcare, Solingen, Germany) at a flow rate of 0.5 ml · min−1 in running buffer (50 mM Tris/HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the eluted protein were pooled and immediately subjected to buffer exchange using HiPrep™ 26/10 desalting column on an AKTA FPLC purification system (GE Healthcare, USA) into storage buffer (50 mM Tris-HCl ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...
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bioRxiv - Bioengineering 2022Quote: We deployed vendor-neutral pulse sequences developed in RTHawk v3.0.0 (rc4-28-ge3540dda19) (HeartVista Inc., CA, USA) on three 3T systems: (G1) GE Discovery 750 software version DV25 (R02_1549.b) (GE Healthcare, Milwaukee, MI, USA), (S1 ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Bacterial lysates were cleared by centrifugation at 10,000 g for 20 min at 4°C and applied to a HisTrap HP column using an AKTA Prime protein purification system (GE Healthcare, Life Sciences). Bound protein was eluted with a linear imidazole gradient ...
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bioRxiv - Neuroscience 2020Quote: ... and hyperphosphorylated 2N4R tau produced in Sf9 cells to LRP1 or the VSP10 domain of SORL1 were assessed using a Biacore 3000 optical biosensor system (GE healthcare Life Sciences) essentially as described (M ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for analyzing the binding of a synthetic GRP78-targeting peptide WDLAWMFRLPVG (GL Biochem Ltd, Shanghai, China) in Biacore X100 SPR system (GE Healthcare, NJ, USA). The system was firstly equilibrated with the running buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... Purification was achieved by immobilized metal affinity purification followed by size exclusion chromatography (SEC) on an ÄktaPurifier Chromatography System (GE Healthcare, Buckinghamshire, UK).
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bioRxiv - Physiology 2020Quote: ... transthoracic echocardiography was performed for cardiac examination using a Vivid 7 dimension cardiovascular ultrasound system equipped with a probe of 9-14 MHz frequency (GE Healthcare, Vélizy, France) as described in the supplemental methods herein ...
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bioRxiv - Physiology 2021Quote: ... on an ÄKTA Pure Protein Purification System equipped with a Superdex 75 10/300 GL gel-filtration column and a 100 µL loop (GE Healthcare Life Sciences). Buffer was 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The blots were developed with the SuperSignal West Femto Chemiluminescent system (Pierce) and visualized with an Amersham Imager 600 (GE Healthcare Life Sciences). Mouse-anti RpoB antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Association and dissociation reactions of GP96 to pneumococcal Ali proteins were analyzed using a BIAcore optical biosensor (BIAcore X-100 system, GE Healthcare Life Sciences), as previously described48 ...
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bioRxiv - Biochemistry 2020Quote: ... and the CBP eluates were further purified by gel-filtration chromatography using a Superdex 200 Increase 10/300 GL column on an ÄKTA Pure purification system (Cytiva, formerly GE Healthcare Life Sciences). The proteins in the peak fractions were collected and concentrated to 1-3 mg/mL for storage at −75°C ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoblots were developed using Chemiluminescent ECL western blotting detection reagents (ECL™Prime Western Blotting System, RPN2232 or ECL Select™ Western Blotting Detection Reagent RPN2235 from GE Healthcare). Images were analyzed on a ChemiDocMP system (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantification of band intensities was carried out on digital images of membrane samples provided by LAS4000 gel documentation system (GE Healthcare; Barcelona, Spain) using ImageJ software (1.50b ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified by affinity chromatography with nickel affinity columns (HisTrap 1 ml; Cytiva) using the Äkta pure system (GE Healthcare UK Ltd.). Elution was carried out at a flow rate of 1 ml/min with all solutions maintained at 4 °C and a linear imidazole concentration gradient from 30 mM to 530 mM.
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bioRxiv - Immunology 2021Quote: ... IgG and IgG-YTE) were expressed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) and purified on HiTrap Protein-A column (GE healthcare, UK).
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitated proteins were analyzed by SDS-PAGE followed by phosphorimaging of dried gels using a Molecular Dynamics Typhoon system with native ImageQuant Software (GE Healthcare, Piscataway, NJ).
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bioRxiv - Cell Biology 2020Quote: ... Localization of mCherry- and GFP-tagged fusion proteins was performed by fluorescence microscopy of live yeast cells using a Visitron spinning disk CSU-W1 (Visitron Systems, Puchheim Germany) or a DeltaVision deconvolution microscope (Applied Precision, Issaquah, WA). The Visitron spinning disk CSU-W1 consisted of a Nikon Ti-E inverted microscope ...
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bioRxiv - Biochemistry 2022Quote: ... were subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column connected to an AKTA PURE 25 FPLC system (GE Healthcare Life Sciences) maintained at 6° C ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
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bioRxiv - Microbiology 2022Quote: ... High performance liquid chromatography (HPLC) was conducted using a HiTrap Q XL 1 mL Sepharose column on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). Proteins were eluted with starting buffer A followed by a gradually increasing concentration of buffer B (20 mM Bis-Tris ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a 5 mL HiTrap Chelating HP Sepharose column on the ÄKTA FPLC protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with 10 mL of starting buffer A (native buffer + 10 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The blots were incubated with γ32-P 5’-labelled probe at 42°C overnight and imaged using a phosphor screen on a Typhoon FLA 7000 Variable Mode Imager System (GE Healthcare, Chicago, IL). Probe sequences are listed in Table S2.
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were further purified by HiTrap Q HP ion-exchange chromatography and Superdex 200 Increase 10/300 GL gel filtration chromatography using an ÄKTApure chromatography system (GE Healthcare Life Sciences). Proteins corresponding to the molecular weight of the tetramerized Vip3Aa were used for subsequent biochemical and bioassay analyses ...
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bioRxiv - Biochemistry 2023Quote: ... Samples previously centrifuged and filtered were run in a Superdex 75 Increase 10/300 GL column connected to an Äkta Pure System (GE Healthcare Life Sciences) equilibrated with buffer 10 mM sodium phosphate ...
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bioRxiv - Bioengineering 2023Quote: All experiments were performed on a 3 Tesla whole-body GE Premier system equipped with a 21-channel receive head coil (GE Healthcare, Milwaukee, WI). We used fluoroptic probes for the gold standard temperature measurement with a precision of 0.1 °C and a temporal resolution of 1 second (STB Medical ...
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bioRxiv - Cancer Biology 2023Quote: The purity of the proteins and their aggregate formation or lack thereof were assessed using a Superdex™ S200 10/300GL SEC column (Cytiva, Vancouver, Canada) and an ÄKTA FPLC™ system (GE Healthcare) in HBS-EP buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Neuroscience 2023Quote: A 56-electrode nylon mesh sock electrode was made using 0.2 mm silver wires and it was placed around the heart to measure the epicardial unipolar electrograms using a Prucka CardioLab electrophysiology mapping system (GE Healthcare, Fairfield, CT) (Figure 4D) ...
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bioRxiv - Microbiology 2023Quote: ... The blots were developed with the SuperSignal West Femto Chemiluminescent system (Pierce) and visualized with an Amersham Imager 600 (GE Healthcare Life Sciences). Mouse-anti RpoB antibody (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed three times with PBS for 10 minutes and subjected to signal detection using ECL™ Prime Western Blotting System (Cytiva) and ImageQuant™ LAS 4000 mini (GE HealthCare). The primary and secondary antibodies were diluted with a PBS solution containing 1% non-fat milk.