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Citations for GE Life Sciences :
1851 - 1900 of 2000 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... equilibrated in protein buffer (10 mM Tris-HCl pH 8.0, 150 mM NaCl) and connected to an Äkta Purifier FPLC system (GE Healthcare, Boston, U.S.A.). The column was washed with five column volumes and subsequently eluted with three column volumes of protein buffer containing 5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2021Quote: Image acquisition of Hoechst 33342 fluorescent nuclei (excitation: 361nm to 497nm; emission 460nm to 490nm) was performed using the INCell analyzer 6500HS automated system (GE Healthcare, USA) at a 10X magnification (Nikon 10X/0.451 ...
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bioRxiv - Microbiology 2020Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy and visualized by SDS-PAGE with silver staining (ProteoSilver™ Plus Silver Stain Kit) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare, Chicago, IL). Fractions were collected and stored at 4°C and the presence of GSTU7-His protein was confirmed by analyzing the collected fractions by SDS-PAGE.
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bioRxiv - Microbiology 2020Quote: ... 300 ml NaCl and eluted via a gradient of increasing imidazole (0-500 mM, Buffer B) using a ÄKTAPurifier™ System (GE Healthcare). After this first purification ...
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bioRxiv - Microbiology 2021Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
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bioRxiv - Cancer Biology 2020Quote: DNA probes complementary to the TP53 coding region or the 3′UTR were labeled with dCTP [α-32P] using the Amersham Megaprime DNA labeling system (GE Healthcare). Primers used for probe synthesis from human cDNA are listed in Supplementary Table 1 ...
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bioRxiv - Biophysics 2020Quote: ... The eluate was concentrated to 500μl and size exclusion chromatography was performed on an ÄKTA pure system (GE Healthcare, Little Chalfont, UK) equipped with a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare) ...
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bioRxiv - Plant Biology 2019Quote: ... cGMP generated from the reactions was measured using the cGMP enzyme immunoassay (EIA) Biotrak System with the acetylation protocol according to the manufacturer’s instructions (GE Healthcare, Illinois USA).
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was quantified using the Alpha Innotech software for the FluoroChemQ chemiluminescent exposure system (Alpha Innotech; GE Healthcare, London, ON, Canada) or ImageLab for ChemiDoc system (BioRad ...
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bioRxiv - Evolutionary Biology 2019Quote: ... gene specific fragments that were used for VIGS (primer pairs listed in Table S1) were radiolabeled with [α-32P] dCTP (Perkin-Elmer) using the Rediprime II DNA Labeling System (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and “Dimeric” RAP was measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols [9,21] ...
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bioRxiv - Neuroscience 2019Quote: ... NF1 participants were scanned at Children’s National Health System on a General Electric whole-body 3T MR scanner (MR750, GE Healthcare, Waukesha, WI). For each participant one or two 6-minute BOLD T2* weighted gradient echo-echo planar imaging protocol (GE-EPI ...
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bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
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bioRxiv - Microbiology 2020Quote: ... and purified to homogeneity on an AKTA FPLC system fitted with a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Rosenfeld et al., 2021), expressed using ExpiCHO™ Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
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bioRxiv - Cell Biology 2020Quote: ... Total internal reflection fluorescent (TIRF) images were acquired using the DeltaVision Elite microscopy system equipped with a multi-line TIRF module (Applied Precision, Inc.), and time-lapse movies were taken at 30-seconds intervals ...
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bioRxiv - Biochemistry 2021Quote: Protein complexes were separated according to relative molecular mass using an Äkta™ FPLC system with a Superdex 200 HR 10/30 column (GE Healthcare) and 24 × 106 cardiomyocytes per sample ...
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bioRxiv - Microbiology 2021Quote: ... The resulting proteins were purified using the AKTA start purification system through DEAE Sepharose Fast Flow packed in the XK16 column (GE Healthcare, USA), followed by another purification using Phenyl Sepharose (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... SEC-MALS runs were performed on an Agilent 1260 Infinity II Prime Bio LC System coupled with a Wyatt miniDAWN® MALS detector using a Superose6 increase 10/300 chromatography column (GE Healthcare) equilibrated with 25 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown in appropriate synthetic dropout media at 30°C to mid-log phase (OD600 of ∼0.5) and imaged using DeltaVision Elite system (GE Healthcare Life Sciences) equipped with an Olympus IX-71 inverted microscope ...
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bioRxiv - Biochemistry 2021Quote: ... The samples (equivalent to ∼85 µg microsomes) were then analyzed with an HPLC system and injected to a Superose 6 column (GE Life Sciences), equilibrated in a running buffer containing 25 mM Tris pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Deutschland) size exclusion chromatography followed by a Superdex 200 Increase 10/300 GL column (ÄKTA Pure system, GE Healthcare, Boston, Massachusetts, USA) purification at 4 °C ...
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bioRxiv - Microbiology 2020Quote: The crude enzyme extract was purified using ion-exchange chromatography (IEC) on a DEAE Sepharose Fast Flow column (5 X 0.5 cm) with an ÄKTA purifier system (GE Healthcare, Munich, Germany). One hundred fifty mL of sample was applied to the column using Buffer A (40 mM citrate-phosphate buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and probed with 5’ end-labeled oligonucleotides or PCR fragments labeled by random priming (Megaprime DNA Labeling System, GE Healthcare Life Sciences). Hybridizations were performed in UltraHyb or UltraHyb-Oligo (Ambion ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Immunology 2022Quote: ... was incubated with or without 200 µM dGTP in a total volume of 50 µl for 5 min prior to size exclusion chromatography (SEC) using an Ettan™ LC System (GE Healthcare). Samples were injected into an analytical Superdex 200PC3.2 column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were run on the gel at 300V for 135 minutes using a gel transfer system (Doppel-Gelsystem Twin L, PerfectBlue).The samples were transferred to a Hybond-XL membrane (GE healthcare, RPN203S) at 50V for 1 hour at 4°C and then cross-linked using 0.12J (UV-lamp T8C ...
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bioRxiv - Microbiology 2023Quote: ... using a 1 ml Ni-NTA FPLC affinity chromatography column (Macherey & Nagel, Germany) coupled to an Äkta Purifier or Prime system (Cytiva/GE Healthcare, U.S.A) at 20°C (room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The chemiluminescence was detected in Fusion FX7 image and analytics system (Vilber Lourmat) after treatment of the membrane with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... HRP activity was visualized by enhanced chemiluminescence with ECL Plus Western Blotting Detection System using a ImageQuant™ LAS imager (GE Healthcare). Band intensity was estimated using the Image J software ...
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bioRxiv - Microbiology 2023Quote: SIM was performed using a v4 DeltaVision OMX 3D-SIM system fitted with a Blaze module (Applied Precision, GE Healthcare, Issaquah, USA) with lasers used to illuminate samples ...
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bioRxiv - Microbiology 2023Quote: SIM was performed using a v4 DeltaVision OMX 3D-SIM system fitted with a Blaze module (Applied Precision, GE Healthcare, Issaquah, USA) with lasers used to illuminate samples ...
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bioRxiv - Biochemistry 2023Quote: ... and further purified by size-exclusion chromatography (SEC) on a Superdex200 16/60 column and ÄKTA Pure system (GE Healthcare, Uppsala, Sweden). The pure BoNT/X-NTNH/X complex was concentrated to 8.4 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The concentrated sample was subjected to size exclusion chromatography using an Äkta FPLC UPC-900 liquid chromatography system (GE Healthcare, Solingen, Germany) equipped with a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... and the immune reactivity was revealed using the ECL chemiluminescence system (SuperSignal, ThermoScientific) and imaged using the Amersham Imager 600 (GE Life Sciences). Optical densities of bands were quantified using the Gel Analyzer plugin in Fiji– ImageJ ...
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bioRxiv - Microbiology 2023Quote: The concentrated affinity-purified protein sample underwent further purification by fast performance liquid chromatography (FPLC) using an AKTA purifier UPC10 system (GE Life Sciences) and a Hiprep 16-60 Sephacryl S300HR size exclusion chromatography column at a flow rate of 1 ml min-1 (running buffer was the same as the binding buffer but without the imidazole) ...
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bioRxiv - Microbiology 2023Quote: ... The amount of protein present in immunoblot bands was quantified using the ImageQuant LAS 4000 system with ImageQuant TL7.0 analysis software (GE Healthcare Life Sciences) or ChemiDoc MP (Bio- Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions of 2 ml were collected at a flow rate of 1 ml/min on an ÄKTA prime Plus system (GE Healthcare Biosciences) equilibrated with buffer F ...
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bioRxiv - Microbiology 2023Quote: The His6-tagged proteins were purified by affinity chromatography using HisTrap™ HP Ni-NTA columns and the ÄKTA purifier liquid chromatography system (GE Healthcare) according to the instructions of the manufacturers ...
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bioRxiv - Biophysics 2023Quote: ... and gel filtration chromatography on a HiLoad 16/60 Superdex 200 column controlled by an AKTA pure FPLC system (GE Life Sciences). The protein was concentrated to ∼3 mg/ml in buffer containing 150 mM NaCl ...
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bioRxiv - Genetics 2023Quote: ... Immunoreactive proteins were detected in a ImageQuant LAS 4000 system with Amersham ECL Prime chemiluminescent reagent according to the manufacturer’s protocol (GE Healthcare Life Sciences). Primary antibodies used in this study were KLF4 (AF3158 ...
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bioRxiv - Microbiology 2023Quote: ... levels present in immunoblot bands were quantified using the ImageQuant LAS 4000 system with ImageQuant TL7.0 analysis software (GE Healthcare Life Sciences) according to the manufacturer’s instructions and normalized to that of EGFP-EF-1δ(F ...
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bioRxiv - Microbiology 2023Quote: ... MilliporeSigma™ Millex™) before loading on a Histrap FF IMAC column (17531901, Cytiva) using the AKTA Purifier 100 FPLC chromatographic system (GE Healthcare). Fractions containing PB1-F2 were pooled and subjected to size exclusion chromatography on a Sepharose S200 column equilibrated with solubilization buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Evolutionary Biology 2023Quote: Native PAGE with Laemmli buffer (pH 8.8) without SDS was performed in 4–20% gradient polyacrylamide gels in a miniVE Electrophoresis System (GE Healthcare, Life Science). High molecular weight standards (Pharmacia ...