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Citations for GE Life Sciences :
1801 - 1850 of 2000 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Microbiology 2022Quote: ... were incubated for 30 min at RT then loaded on a Superdex 200 Increase 10/300 GL column connected to an AKTA purification system (GE Healthcare). The column (bed volume 24 ml ...
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bioRxiv - Molecular Biology 2023Quote: The binding of anti-RBD antibody in post-immunization mice sera to the yeast purified RBD was analysed by SPR using a Biacore T200 system (GE Healthcare) at 25°C ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was filtered on 0.45 µm units before loading onto a nickel-Sepharose Fast Flow column (1mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The PLP protein was eluted at 160 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... Signals were detected with a Fujifilm ImageQuant LAS 4000 mini CCD camera system and quantifications were performed with ImageQuant TL software (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Protein purification was conducted using a combination of affinity and size-exclusion chromatography (SEC) performed using Äkta Purification systems (GE Healthcare). Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... equilibrated with buffer A (50 mM Tris-HCl at the appropriate pH, 300 mM NaCl) on an AKTÄ explorer 10S FPLC system (GE Healthcare). The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography procedures were carried out at 4°C using either an ÄKTA express or ÄKTA pure chromatography system (GE Healthcare). 1 mL StrepTrapTM HP (cat# 28907546 ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Microbiology 2023Quote: SEC analysis was performed using a Superdex 75 10/300 GL prepacked column connected to ÄKTA pure 25 chromatography system (GE Healthcare). The column was equilibrated with a 50 mM potassium phosphate buffer (pH 7.0) ...
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bioRxiv - Biochemistry 2022Quote: ... injected (5 mL total) into HiLoad 16/600 Superdex 75pg (Cytiva) connected to an AKTA FPLC system (GE Healthcare Life Science). Gel filtration was performed using the same buffer to purify Nurr1-RXRα LBD heterodimer for analysis ...
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bioRxiv - Microbiology 2023Quote: ... In separate reactions the resulting PCR fragments were coupled with an alkaline phosphatase enzyme using the Amersham Gene Images AlkPhos Direct Labelling and Detection System (GE Healthcare), according to the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... and the protein was eluted with an imidazole gradient (300 mM-500 mM) using the AKTA prime chromatography system (GE Healthcare). Fractions containing pure ParR protein (26.5 kDa ...
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bioRxiv - Biochemistry 2023Quote: ... then ACKR3 was purified by size exclusion chromatography (SEC) using a Superdex 200 Increase (10/300 GL column) connected to an ÄKTA purifier system (GE Healthcare) and eluted in buffer elution buffer without Flag peptide or VUF11207 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluate was then further purified by size exclusion chromatography using a Superdex 200 Increase (10/300 GL column) connected to an ÄKTA purifier system (GE Healthcare) in a buffer containing 20mM Hepes (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... stored in sample loops on the system and injected into a Gel Filtration column (HiLoad 16/60 Superdex 200 prep grade, GE Healthcare). The protein sample was concentrated using Vivaspin 20 filter concentrators (VivaScience ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Biophysics 2023Quote: ... Samples were injected on the Äkta prime plus chromatography system (Cytivia) and separated on a Superose 6 10/300 column (GE Healthcare) in SEC buffer (10 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified by anion exchange (HiTrap Q, Cytiva) or SEC (Superdex 200 Increase 10/300 GL, Cytiva) chromatography at ÄKTA FPLC (system ÄKTA pure; GE Healthcare). The purity of the protein was checked by the SDS PAGE.
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bioRxiv - Cell Biology 2023Quote: ... and in a second step by immobilized metal affinity chromatography (IMAC) using HisTrap FF Crude columns (Cytiva, Marlborough, MA, USA) in combination with the ÄKTApurifier system (GE Healthcare). The purified recombinant desmin was stored at -80 °C.
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were filtered through 1.2 μm syringe filters and loaded onto the AKTA Xpress system (GE Healthcare, Fairfield, CO, USA). The purification regime is briefly described as follows ...
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bioRxiv - Biophysics 2023Quote: Ion exchange chromatography (IEC) was performed using an ÄKTA Purifier protein purification system controlled by the software Unicorn 3.10 (GE Healthcare, Sweden). CIMmultus QA 1 mL column (BIA Separations ...
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bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
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bioRxiv - Biophysics 2023Quote: Size exclusion chromatography (SEC) was performed using ÄKTAprocess 6 mm Std system controlled by the Unicorn 7.0 software (GE Healthcare, Sweden). Sepharose 6 Fast Flow beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Cell Biology 2023Quote: SEC-MALS experiments were carried out on DAWN HELEOS-II (Wyatt Technology Corporation) coupled with a Shimadzu liquid chromatography system equipped with a Superdex 200 10/300 GL (GE Healthcare) gel-filtration column ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then subjected to size exclusion chromatography in 20 mM tris pH 8.0 using a Superdex-75 HR 10/30 column fractionating at a flow rate of 0.5 mL/min and attached either to an AKTA FPLC system (GE Healthcare, Buckinghamshire) or a BioLogic DuoFlow system (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: Interaction experiments using surface plasmon resonance (SPR) of ADAH11 nanobody monomer and different RBDs were carried out with a Biacore T200 system (GE Healthcare) according to the manufacturer’s protocols and recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Bioengineering 2023Quote: ... The supernatant loaded to the column and purification was performed by using ÄKTA start protein purification system (GE Healthcare Life Sciences) according to the preferred protocols provided by the purification column manual ...
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bioRxiv - Biophysics 2023Quote: ... The peak associated with pure ACBP was concentrated and further purified by SEC using a Superdex 75 (HR 10/30) column coupled to an Äkta purifier system (GE Healthcare) using Buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... the tagless protein was purified with ion exchange chromatography with HiTrap DEAE Sepharose Fast Flow anion exchange column (Cytiva) with Akta Pure liquid chromatography system (GE Healthcare) using linear 0-1 M NaCl gradient in 50 mM Tris-HCl (pH 7.75) ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Immunology 2023Quote: ... with 500 mM NaCl in phosphate-buffered saline (PBS) on an Äkta Purifier 10 high-performance liquid chromatography system (GE Healthcare) to remove associated LPSs ...
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bioRxiv - Plant Biology 2024Quote: ... Immunodetection was performed using an ECL Plus Western Detection Kit and revealed with an ImageQuant LAS 4000 mini CCD camera system (GE Healthcare). ImageJ was used for quantifications of the bands.
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bioRxiv - Bioengineering 2024Quote: - early post-TAVI Transthoracic Echocardiography (TTE) data obtained with commercially available equipment (iE33 or Epiq, Philips Medical System, or Vivid-9, GE Healthcare) [3] between 2 days and 7 months after the implantation ...
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bioRxiv - Pathology 2024Quote: ... 50 µL of sample were injected on a HPLC system (Alliance, Waters) and separated on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... and followed by size-exclusion chromatography (SEC) using a superdex 200 Increased 10/300 chromatography column on an AKTA Purifier 10 FPLC system (GE Healthcare) with buffer containing 20 mM Hepes ...
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bioRxiv - Biochemistry 2024Quote: ... 45 μl was spotted to a double-membrane system on a dot-blot apparatus with an upper nitrocellulose membrane and a lower hybond-N+ membrane (GE Healthcare). After filtration under vacuum ...
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bioRxiv - Cell Biology 2024Quote: ... These eluted proteins were subsequently passed through a superdex s75 16/60 column attached to an AKTA Pure system (GE healthcare) in 50 mM Tris pH 8.0 and 300 mM NaCl.
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bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: ... the protein solutions were exchanged with a 20 mM sodium phosphate buffer (Na2HPO4/NaH2PO4) at pH 7.4 using a HiTrap® Desalting Column connected to an Äkta purifier system (GE Healthcare). Subsequently ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Genomics 2020Quote: Time-lapse imaging of stable RPE1 cells expressing 53BP1-eGFP and Lifeact-RFP was performed in a 37°C climate chamber attached to a Deltavision microscope system and the SoftWorx 4.9 software (Applied Precision/GE Healthcare) using a 40x 1.3 NA oil-immersion objective ...
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bioRxiv - Cancer Biology 2019Quote: ... Eluted material was characterized and further purified by size-exclusion chromatography (SEC) on an ÄKTA Pure system using Superdex 200 columns (GE Life Sciences). Correctly assembled ...
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Biochemistry 2020Quote: ... was used and immunoreactive bands were detected by chemiluminescence (SuperSignal West Pico PLUS, Thermo Fisher or ECL Prime Western Blotting System, GE Healthcare).