Labshake search
Citations for GE Life Sciences :
1601 - 1650 of 2000 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Titrations were performed at 25°C with a stirring speed of 750 rpm using the MicroCalTM iTC200 System (GE Healthcare). A total of 20 injections with 2 μL each and a spacing of 180 seconds were titrated into the 200 μL protein sample (25 mM Hepes pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
-
bioRxiv - Physiology 2022Quote: ... body composition was measured by dual-energy X-ray absorptiometry (DXA) scans (Lunar iDXA, GE Healthcare, GE Medical systems, Belgium). Then ...
-
bioRxiv - Molecular Biology 2021Quote: SEC-SAXS data for the NKR-P1:LLT1 complex were collected at the Diamond Light Source (Didcot, UK) at beamline 21 using an Agilent 1200 HPLC system with 2.4 mL Superdex 200 column (GE Healthcare), a Pilatus P3-2M detector ...
-
bioRxiv - Molecular Biology 2020Quote: ... was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare). 500 μL lysate (2 mg protein ...
-
bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
-
bioRxiv - Cell Biology 2019Quote: ... SPLIFF and other time-lapse experiments were observed with a DeltaVision wide-field fluorescence microscope system (GE Healthcare, Freiburg, Germany) provided with a Olympus IX71 microscope ...
-
bioRxiv - Microbiology 2019Quote: ... The sample was loaded on a MonoQ 4.6/100 PE anion exchange column run on an ÄKTApurifier FPLC system (both GE Healthcare) with following parameters ...
-
bioRxiv - Genetics 2019Quote: ... we used the anti-rabbit HRP-conjugated antibody (1/5000 dilutions) with the ECL Western Blotting Detection System (GE Healthcare) according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2019Quote: ... Germany) reconstituted in water was run on a Superdex 200 increase 10/300 column /Äkta Pure system (GE healthcare, Germany) equilibrated with 20mM Tris ...
-
bioRxiv - Biochemistry 2019Quote: ... Crosslinked peptide fragments were enriched by SEC using a Superdex Peptide PC 3.2/30 column on an AKTA pure system (GE Healthcare) at a flow rate of 50 μL/min ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... and adjusted to pH 8.0 in 10 mM Tris before purification in an AKTA Avant system by Steptrap-HP chromatography (GE Healthcare). Final purification was achieved by gel filtration using a Superdex 75 HiLoad 16/60 column (GE Healthcare ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare). Fractions were collected and stored at 4°C.
-
bioRxiv - Biochemistry 2020Quote: ... The His6-tag-containing FdR9 was purified by affinity chromatography on an Äkta prime FPLC system (GE Healthcare, Freiburg, Germany), using a 5 mL HisTrap column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The target protein was cleaved off the column with TEV protease and subjected to size exclusion chromatography on a Superose 6 Increase 3.2/300 column connected to the Ettan system (GE Healthcare) operated at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a 1-ml HisTrap column connected to an ÄKTA Explorer 100 purification system (GE Healthcare), previously equilibrated with Buffer A ...
-
bioRxiv - Bioengineering 2021Quote: ... A final polishing step was performed by 200 kDa size exclusion chromatography on AKTA pure chromatography system (GE Life Sciences) to remove α-lactose and further isolate IDO-Gal3 ...
-
bioRxiv - Bioengineering 2021Quote: Affinity of IDO-Gal3 for lactose was determined using affinity chromatography in an AKTA Pure chromatography system (GE Life Sciences) equipped with consumer-packable glass column (GE Life Sciences ...
-
bioRxiv - Bioengineering 2020Quote: ... Size-exclusion chromatography was performed on an ÄKTA FPLC system using a Superdex 200 increase 10/300GL column (GE Healthcare). Protein binding affinity was performed by Surface Plasmon Resonance using a BIAcore X100 instrument using a CM5 chip coated with recombinant fibronectin 11A12 domain ...
-
bioRxiv - Biochemistry 2021Quote: ... the bound fusion protein was eluted with 20– 200 mM imidazole gradient using a FPLC purifier system (ÄKTA; GE Healthcare). Peak fractions were pooled and digested with SENP2 protease (at final 0.01 mg/ml ...
-
bioRxiv - Biochemistry 2020Quote: ... and 500 μL (4 μg BSA) was injected into an AKTA™ PureL system (GE Healthcare Life Sciences, Chicago IL), and run over a Superdex 200 GL (10/300 ...
-
bioRxiv - Cell Biology 2021Quote: ... which was performed at room temperature on an ÄKTA Pure 25L1 system with a Superdex 200 Increase column (GE Healthcare). Elution of proteins was monitored at 280 nm ...
-
bioRxiv - Biochemistry 2021Quote: Live-cell analysis was performed on a Deltavision Elite system using a × 40 oil objective with a numerical aperture of 1.35 (GE Healthcare). The DeltaVision Elite microscope was equipped with a CoolSNAP HQ2 camera (Photometrics) ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
-
bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.
-
bioRxiv - Plant Biology 2020Quote: ... in a high-salt buffer containing 20 mM Tris-HCl (pH 8.0) and 50 mM NaCl in an ÄktaPrime or ÄktaExplorer system (GE Healthcare). The column was calibrated with bovine erythrocyte carbonic anhydrase ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody was expressed using ExpiCHO expression system (LifeTechnologies) according to the manufacturer’s protocol and purified using a Protein A MabSelect SuRE column (GE Healthcare). The wash buffer contained 20mM Tris & 150mM NaCl buffered to pH 8.6 and the elution was done using 0.1 M Citric acid pH 2.5 ...
-
bioRxiv - Cell Biology 2020Quote: ... Final polishing was performed by gel-filtration on a Superdex 200 16/60 coupled to an AktaPurifier system (GE Healthcare) into Binding Buffer (50 mM HEPES ...
-
bioRxiv - Biophysics 2022Quote: ... pre-equilibrated with buffer (50 mM HEPES pH 7.4 and 0.08% [w/v] GDN) was used to exchange CIV into GDN using an Äkta Pure M25 chromatography system (GE Healthcare) operated at 4°C with UV detection at 280 nm and 420 nm ...
-
bioRxiv - Plant Biology 2022Quote: ... strain BL21 as described previously,20 using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... and passed through a 0.45-μm filter prior to protein purification via affinity chromatography using an AKTA Start FPLC system (GE Healthcare). The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... Gels were dried and then imaged using a phosphor storage screen and Typhoon Trio Variable Mode Imager System (GE Healthcare). Extent of modification was estimated by band intensity comparison using ImageQuant TL 1D Version 7.0.
-
bioRxiv - Microbiology 2022Quote: ... SPR measurements were recorded at 20 °C using a Biacore 8k system using a ReDCaT SA sensor chip (GE Healthcare) with 8 immobilised channels as described in (43) ...
-
bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTA micro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... Placement in the spinal cord was confirmed with a commercially-available ultrasound system (LOGIQ e Vet, GE Healthcare, Fairfield, CT) using a linear array 4-10 MHz transducer (8L-RS) ...
-
bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The solution was then applied to a HisTrap FF crude column on an Aekta pure system (GE Healthcare, Freiburg, Germany), extensively washed with His-A buffer ...
-
bioRxiv - Zoology 2021Quote: Transthoracic echocardiographic examinations were performed in the orangutans’ indoor enclosure in the Ménagerie, le Zoo du Jardin des Plantes (Paris, France) using a portable cardiovascular ultrasound system (Vivid i, GE Healthcare, 9900 Innovation Drive ...
-
bioRxiv - Neuroscience 2022Quote: ... or nitration/phosphorylation were loaded to a final concentration of 36 ng into the Slot Blot system (GE Healthcare, Switzerland). The system was assembled with a pre-wetting nitrocellulose membrane and two Whatman filters in PBS for 10 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a Superdex 200 10/300 GL column using an ÄKTA chromatography system (GE Healthcare Life Sciences). 0.5 ml or 1 ml fractions were collected and analyzed by Western blotting using antibodies against HOIP and V5.
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTAmicro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
-
bioRxiv - Biochemistry 2020Quote: ... using a linear gradient of buffer B to increase the concentration of imidazole on an ÄKTA purifier system (GE Healthcare). The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by IMAC purification on an Äkta purifier chromatography system using a 5ml HisTrap Excel column (GE Healthcare, Freiburg, Germany). The column was washed by a linear imidazole gradient (20-70 mM) ...
-
bioRxiv - Microbiology 2020Quote: ... The protein content during the washing and elution steps was monitored using the Äkta purifier 100 system (GE Healthcare, UK) and double-checked with a Bradford assay ...
-
bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
-
bioRxiv - Genetics 2019Quote: ... The probe label and signal detection were performed using the Gene ImagesTM AlkPhos DirectTM labelling and detection system (GE Healthcare), according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography was performed on a quaternary Agilent 1290 Infinity II UPLC system equipped with a SEC column (Superdex Peptide 3.2/300, GE healthcare). 40 μg of the eight protein mixture was loaded and separated at a flow rate of 0.05 ml/min with running buffer (30%ACN ...