Labshake search
Citations for GE Life Sciences :
1851 - 1897 of 1897 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... Gel bands corresponding to the pure target single-strands were identified by UV-shadowing and subject to electroelution (Whatman, GE Healthcare, in TAE buffer) followed by ethanol precipitation ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... Proteins (20-30 μg) were separated on 10% SDS-PAGE gels and blotted to a 0.45-μm pore nitrocellulose filter (GE Healthcare, Europe GmbH, Freiburg, Germany) using Trans Blot Turbo system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... 75 V) and gel image was taken using an Image Quant LAS 500 chemo luminescence CCD camera (GE healthcare, life sciences, Chicago, IL, USA).
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bioRxiv - Neuroscience 2019Quote: ... Equivalent amounts of protein or equal volume from each sample were run on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (GE Healthcare, Piscataway, NJ, USA). Following blocking in 5% milk or bovine serum albumin the blots were incubated with primary antibodies and analyzed by HRP-conjugated secondary antibodies (Jackson Laboratories) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were further resolved using size exclusion chromatography on gel filtration columns using the Superdex 200 (16/600) (GE Healthcare, GE28-9893-35) or Superdex 75 (16/600 ...
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bioRxiv - Microbiology 2020Quote: The content of IgG monomers was assessed by FPLC gel filtration chromatography in a Superdex 200 10/300 GL column (GE Healthcare, Pharmacia; Stockholm, Sweden), using 0.15 mol/L NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... DNA fragments were separated on the 0.8% agarose gel and alkali-blotted onto a Hybond XL membrane (GE Healthcare Life Sciences, Waukesha, WI, USA). Telomere fragments were detected by a radioactively labeled telomeric DNA probe ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... Size-exclusion chromatography was performed on a 24 ml Superdex-75 gel filtration column on an ÄKTA Purifier System (GE Healthcare, Chicago, Illinois, USA) using the same buffer as during sedimentation velocity measurement ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were separated via 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis for 90 min and transferred to polyvinylidene fluoride blotting membranes (0.2 µm pore size; GE Healthcare, Chalfont St. Giles, UK) for 2 h ...
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bioRxiv - Physiology 2022Quote: ... A final washing step was completed and then membranes were developed with enhanced chemiluminescence (ECL) reagent and imaged using an LAS4000 Gel Imager with ImageQuant densitometry software (GE Healthcare, Mississauga, ON, Canada).
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and 85% of this suspension (containing membrane-cytoskeleton fraction) was loaded onto a Superdex 200 10/300 Gel Filtration column (GE Healthcare Life Sciences, UK) equilibrated with 20 mM Tris pH 7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μg of total RNAs were separated on 10% polyacrylamide–8M urea gels and transferred onto a nylon membrane (GE Healthcare Amersham Hybond −N+). The membrane was then UV cross-linked and dried at 80°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were separated on an SDS-15% polyacrylamide gel and then electrophoretically transferred onto the nitrocellulose membrane Amersham Protran 0.45 µm NC (GE Healthcare Lifesciences, Chicago, IL, USA). Membrane-immobilized proteins were probed with mouse anti-linker histone H1 monoclonal antibody clone AE-4 (Upstate Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were electrophoresed on an SDS polyacrylamide gel and then transferred to a nitrocellulose membrane (Amersham Protran Premium; GE Healthcare, Waukesha, WI, USA) using a semidry method ...
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bioRxiv - Molecular Biology 2022Quote: ... run in MES buffer for 45 min at 200 V and the gel was imaged using a Typhoon 9500 (GE healthcare, 600 V power).
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of total RNAs were fractionated by formaldehyde-agarose gel electrophoresis and transferred to Amersham™ Hybond-XL nitrocellulose membrane (GE Healthcare, Cat# RPN203S) by capillarity ...
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bioRxiv - Cell Biology 2023Quote: ... estimated by Bradford assays) were separated in a 10 % (w/v) polyacrylamide gel and then transferred on PVDF membranes (GE Healthcare Life Sciences, Amersham). Immunodetection was performed with an anti-HA monoclonal antibody [HA Epitope Tag (YPYDVPDYA ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then dried at 80°C for 45 min and exposed overnight to a storage phosphor screen (GE Healthcare, BAS-IP SR 2025), which was subsequently imaged with Typhoon FLA700 gel scanner and quantified in Fiji/ImageJ.
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bioRxiv - Biochemistry 2023Quote: ... and Rgl2RA were mixed in the GF buffer and the complex was purified on SEC using a gel-filtration column (GE Healthcare, HiLoad Superdex 75). The peak fractions containing both proteins in the 1:1 ratio were collected and concentrated to set up crystallization screenings ...
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bioRxiv - Biochemistry 2023Quote: ... and filtrated using a centrifugal filter unit (Milipore, Ultrafree, #UFC30GV00) before conducting size exclusion chromatography (SEC) with a gel-filtration column (GE Healthcare, HiLoad Superdex 75) attached to an FPLC system ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Molecular Biology 2019Quote: ... approximately 30 μg of total RNA was denatured and separated on a 1% denaturing formaldehyde agarose gel and transferred to a Hybond-N+ nylon membranes (GE Healthcare Life Sciences, London, UK). PCR products were labeled with DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were then extracted and purified using an illustra™ GFX™ PCR DNA and Gel Band Purification kit (GE Healthcare, Buckinghamshire, UK). The samples were sequenced using the general Sanger sequencing technique.
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bioRxiv - Molecular Biology 2020Quote: ... was purified by gel filtration on a Superdex-200 size-exclusion chromatography column connected to an ÄKTA FPLC apparatus (GE Healthcare Bio-Sciences, Piscataway, NJ) with an elution buffer comprised of 25 mM Tris (pH 8) ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 10,000 xg for 20 min and the His6-tagged-proteins were purified by Ni-NTA chromatography followed by size exclusion gel filtration using Superdex 200 10/300 GL column (GE Healthcare Life Sciences, Pittsburg, PA).
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bioRxiv - Neuroscience 2023Quote: ... Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029). Membranes were incubated in Fisher No-Stain Total Protein Stain (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting using the ECL Select Western Blotting Detection System (GE Healthcare Japan Co., Ltd., Tokyo, Japan) and a LAS-3000 imaging system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029) or 0.45µm Nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and purity of these samples were evaluated on a 1% agarose gel and on a NanoVue spectrophotometer (GE Healthcare Life Sciences, Chicago IL, US). We used an Illumina paired-end TruSeq RNA library preparation kit for 150 sequencing cycles for a mid-output flowcell ...
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bioRxiv - Neuroscience 2024Quote: ... Twenty µg retinal samples and 15µg nerve samples were loaded onto SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029). Membranes were incubated in Fisher No-Stain Total Protein Stain (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... reactions were diluted with water (970 μl) and purified by desalting on a disposable gel-filtration column (NAP10, GE Healthcare Life Sciences, cat. no. GE17-0854-02) according to the manufacturer’s instruction to remove the majority of the free dye ...
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Biophysics 2022Quote: ... with salt gradient of (250-1000mM NaCl for PARP1 and 50mM-1000mM NaCl for all other constructs) and finally through gel filtration (16/600 200pg or 16/600 75pg, GE Healthcare, depending on the size of the protein) column pre-equilibrated with buffer 150mM NaCl ...