Labshake search
Citations for GE Life Sciences :
1451 - 1500 of 1957 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biochemistry 2021Quote: ... and concentrated by ultrafiltration (Amicon, 10 kDa cutoff,) before further purifying on a Superdex S75 16/60 gel filtration column (GE Healthcare) in 25 mM NaH2PO4/Na2HPO4 pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to nitrocellulose (GE Healthcare. For preparation of cell wall extracts for mass spec ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration chromatography was performed using an AKTA FPLC System with a Superose 6 Increase 10/300 GL column (GE Healthcare) in 50 mM NaPO4 pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2020Quote: ... heated to 95°C for 5min and separated on a 10% SDS-PAGE gel before transferring onto a nitrocellulose membrane (Amersham Hybond ECL, GE Healthcare) using BioRad transfer apparatus ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Microbiology 2021Quote: ... The digested proteins were then passed over a 5mL HisTrap-FF column and the flow-through was collected for further purification by gel filtration chromatography using a HiLoad Superdex 75 26/600 pg column (GE Healthcare). The monodisperse peak corresponding to BBK32-C was pooled following analysis of chromatography fractions by SDS-PAGE and exchanged into HBS (10mM HEPES (pH 7.3) ...
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bioRxiv - Molecular Biology 2020Quote: ... The gel was stained in Sybr Green II and scanned on a Typhoon 9410 Variable Mode Imager (GE Healthcare, Piscataway, NJ).
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bioRxiv - Molecular Biology 2020Quote: ... and harvested from cell culture medium followed by purification procedure using Ni-NTA column and Superdex 200 gel filtration column (GE Healthcare) in tandem ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples of TRIM21 CC 235 (100uL) were resolved on a Superdex S-200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min in 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Gels were dried and analyzed using the Typhoon FLA 7000 phosphor-imager and the ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... and 10-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1ko blot) Bis-Tris gels (Thermo) before transferring to PDVF membranes (GE Healthcare). Membranes were blocked in 5% milk and blotted with primary and secondary HRP antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The PAGE gel was exposed to a phosphosimage screen for ∼2 hours and analyzed using a Amersham Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: All the gel filtration chromatograms were ran in a Superdex 200 increase 10/300 column (fractionation range 10-600 kDa, GE Healthcare) coupled to an AKTA purifier ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) each ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein can be further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) as described above.
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bioRxiv - Molecular Biology 2019Quote: ... 10 µg of total RNA was size separated on an 18% denaturing polyacrylamide gel and subsequently blotted onto an Amersham HybondTM-N+ membrane (GE Healthcare) and cross-linked ...
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bioRxiv - Biophysics 2022Quote: ... and 5mg/mL (NNDP52, CNDP52 and ZF2) were loaded onto a Superdex 200 (30 x 1cm) analytical gel filtration column (GE Healthcare), equilibrated in 50mM Tris pH7.5 ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The gel was set onto a photostimulatable storage phosphor screen and the signal was detected by the Typhoon 7000 phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing EftMHM4 or EftMPAO1 were pooled and the protein further purified by gel filtration chromatography on a Superdex200 10/300 column (GE Healthcare) equilibrated with gel filtration buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was eluted by using purification buffer including 200 mM imidazole and further purified by gel chromatography on an ÄKTA pure system equipped with a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) running with purification buffer ...
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bioRxiv - Biochemistry 2020Quote: ... All products were resolved on 12% polyacrylamide gels containing 8 M urea and visualized using Typhoon Trio fluorescence imager (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The protein samples were centrifuged at 12,000g for 10 min to remove any protein precipitation and then loaded into a gel filtration column (Superdex 200 Increase 10/300GL, GE Healthcare) coupled with an 18 angle light scattering detector (DAWN HELEOS II ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing Mtb UgpB were pooled and loaded onto a Supderdex 75 pg HiLoad 16/600 gel filtration column (GE Healthcare) and eluted with buffer C (25 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... Products were separated by gel electrophoresis (20 % denaturing polyacrylamide) and visualised by exposure to a BAS Storage Phosphor Screen (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... The gels were exposed to phosphor screens and the autoradiographs were developed in a Typhoon FLA 9500 (GE Healthcare Life Sciences). The oligonucleotides used are listed in Table S2 (SI).
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... it was purified on a nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Visualization of PAGE gels was performed by fluorescence imaging using a Storm 860 or a Typhoon Trio phosphorimager with the ImageQuant software (GE Healthcare). Natural DNA and RNA oligonucleotides were purchased from Microsynth ...
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bioRxiv - Biochemistry 2019Quote: ... of total cellular protein was separated by SDS-PAGE using 4-12.5 % Bis-Tris gels and transferred to nitrocellulose membrane (GE Healthcare # 10600006). SeeBlue pre-stained protein standard (Invitrogen # LC5925 ...
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bioRxiv - Biochemistry 2019Quote: Peak fractions from the IEX were concentrated to < 4 ml and loaded onto a Superdex 16/60 gel filtration column (GE Healthcare) in Buffer A ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the desired protein were pooled and gel filtration was performed on Superdex 75 or 200 10/300 GL columns (GE Healthcare) mounted into an ÄKTA P100 chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... before heating at 95°C for 5 min and samples were separated by SDS-PAGE using a 4-16% acrylamide gradient gel and blotted onto nitrocellulose membranes (Hybond-C Extra, Amersham Bioscience or Optitran BA-S 85, GE Healthcare). The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako ...
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bioRxiv - Molecular Biology 2019Quote: ... Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl) and the resulting histone octamer purified using a Superdex 200 gel filtration column (GE Healthcare). Purified 147bp DNA carrying the 601 nucleosome-positioning sequence was a kind gift from the Brockdorff lab ...
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bioRxiv - Biochemistry 2019Quote: ... The HiTrap ButylFF column of 1 mL and the gel filtration column Superdex 200 10/300 GL were from GE Healthcare. MacroPrep HighS resin was from Biorad ...
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bioRxiv - Cancer Biology 2019Quote: ... All fractions identified to contain cleaved RNF114 were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer ...
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bioRxiv - Immunology 2020Quote: ... The Biotin-labelled-Avi-His6-SUMO proteins were purified using a nickel-NTA column followed by gel-filtration chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare). Mouse and human TBK1 (residues 1 to 657 ...
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bioRxiv - Microbiology 2019Quote: Samples were electrophoresed on a 15% SDS polyacrylamide gel and proteins were then blotted onto a nitrocellulose membrane (GE Healthcare, Amersham). Nitrocellulose was blocked with 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2019Quote: ... electrophoretically separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences, 10600003). Total protein bands were reversibly stained with Fast Green FCF (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction products were electrophoretically separated on a denaturing 24% polyacrylamide/7 M urea gel and visualized with a phosphorimager (Typhoon 9410; GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... from mouse liver was separated by 10% denaturing polyacrylamide gel electrophoresis (PAGE) and blotted onto a nylon membrane (Amersham Hybond N+; GE Healthcare) using a Transblot Turbo apparatus (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were resolved by SDS-PAGE using a 10 % acrylamide gel and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Amersham Hybond, GE Healthcare) via the PerfectBlue™ tank electro blotter (Peqlab ...
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bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
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bioRxiv - Biophysics 2021Quote: ... and 20 mM NaCl and subjected to gel filtration using the same buffer on a Superdex 200 column attached to an ÄKTA FPLC (GE Healthcare) at 4 °C ...