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Citations for GE Life Sciences :
1551 - 1600 of 1897 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The dried gels were exposed to a storage phosphor screen and radioactive signals were detected with a Typhoon biomolecular imager (GE Healthcare). Signals were quantified using ImageJ64 software (NIH).
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bioRxiv - Microbiology 2019Quote: ... Orn was concentrated by Amicon Ultra 10K concentrator prior to loading onto a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Biophysics 2019Quote: ... The eluted protein fraction was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) in the buffer of 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2019Quote: ... All fractions identified to contain the cleaved protein were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer (20 mM Bis-Tris pH 7.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Cell Biology 2019Quote: ... PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with gel filtration buffer (20 mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2019Quote: ... Southern analysis was performed by separating DNA fragments in 1% agarose/1X TBE gels using pulse-field electrophoresis with a 5-45 s ramp and blotted onto Hybond-XL membranes (GE Healthcare) with alkaline transfer.
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bioRxiv - Cell Biology 2020Quote: ... The 176 bp GUB DNA was recovered from the supernatant of the polyethylene glycol precipitation fraction and further purified with gel-filtration chromatography on a HiLoad 16/60 Superdex 75 pg column (GE Healthcare) using TE buffer (10 mM Tris-HCl [pH 7.5] and 0.1 mM EDTA) ...
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bioRxiv - Cell Biology 2020Quote: ... and H3–H4 tetramer and H2A–H2B dimer were isolated with gel-filtration chromatography on a HiLoad 16/60 Superdex 75 pg column (GE Healthcare). Fractions containing (H3–H4 tetramer and H2A– H2B dimer ...
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bioRxiv - Microbiology 2020Quote: ... The protein in the supernatant was sequentially purified using a Ni-NTA column and a Superdex200 gel filtration column (GE Healthcare) as previously described (20) ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
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bioRxiv - Cell Biology 2020Quote: ... MFG-E8-eGFP was further purified by gel filtration on an Äkta prime system with a Superdex 200 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... 10 mM DTT and the flow-through (containing PFN1) was collected and then applied to a Superdex 75 (10/300) gel filtration column (GE Healthcare) equilibrated in 50 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: ... Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG ...
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bioRxiv - Microbiology 2020Quote: ... Gels were dried and exposed to PhosphorImager screens overnight (approximately 17 hours) and imaged with a Typhoon-9410 scanner (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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bioRxiv - Synthetic Biology 2020Quote: ... The gel filtration was performed with a HiPrep 16/60 Sephacryl S-100 column on an AKTA chromatography system (GE Healthcare), and the size of the proteins in each eluted fraction were verified by SDS-PAGE.
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bioRxiv - Molecular Biology 2020Quote: ... The gels were visualized by the 6-FAM label on the RNA primer using the Typhoon 9500 FLA Imager (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Biochemistry 2020Quote: ... The elute was concentrated to 5 ml and applied to a gel filtration run on a 16-600 HiLoad Superdex 200 pg column (GE Healthcare), preequilibrated with 20 mM Hepes (pH 7.0) ...
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bioRxiv - Biochemistry 2020Quote: ... gels were dried and exposed with Super RX Medical X-Ray Film (FUJI) or scanned using a Typhoon phosphoimager (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM EDTA (pH 6.8) prior to purification by size exclusion chromatography using a gel filtration column (HiLoad 26/60 Superdex 75 column, GE Healthcare). The sample was concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Final gels were exposed to a storage phosphor screen for 1 hour and scanned using a Typhoon Imager 9410 (GE Healthcare). Band intensities were determined using the Fiji ImageJ software49 ...
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bioRxiv - Biophysics 2020Quote: ... The protein in the supernatant was collected and subjected to gel filtration chromatography in a Superose6 10/300 GL column (GE Healthcare) previously equilibrated in Buffer B1 with 0.02% DDM ...
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bioRxiv - Microbiology 2020Quote: Molecular mass determination was carried out using gel-filtration chromatography with a Superose 6 Increase 10/300 GL (GE Healthcare, USA) column ...
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bioRxiv - Microbiology 2020Quote: ... MlaFB containing fractions eluted from the NiNTA column were pooled and concentrated before separation on a Superdex 200 16/60 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA was loaded per well on a denaturing agarose gel and was blotted via capillary blot to Hybond(tm)-N+ membrane (GE Healthcare). The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... applying a gradient of imidazole from 50 mM to 500 mM and further separated by gel filtration using an HiLoad 16/600 Superdex 200 (GE Healthcare) in equilibration buffer ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated by protein electrophoresis at 120 V for 2 h using 10% Bis-Tris pre-cast gels (NuPage) and transferred overnight at 4°C onto Hybond-P PVDF membranes (GE Healthcare). After blocking in ECL blocking agent (GE Healthcare) ...
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...
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bioRxiv - Immunology 2020Quote: ... 30 μg of total protein were separated on 4-12% Bis-Tris polyacrylamide gels (NuPAGE, Invitroge) in MES SDS running buffer then blotted onto Protran 0.2 mM Nitrocellulose (GE Healthcare, UK). Membranes were probed with 1:1000 dilution of the appropriate primary antibody anti-pSer727-STAT3 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... was further purified by cation exchange and heparin affinity chromatography prior polishing by gel filtration through a Superdex 200 16/40 column (GE Healthcare), which was operated in 300 mM NaCl ...
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bioRxiv - Pathology 2019Quote: ... proteins were separated based on their isoelectric point on 18-cm immobilized pH gradient (IPG) gel strips of pH 3-10 (GE Healthcare). Subsequent to overnight rehydration ...
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bioRxiv - Genetics 2019Quote: ... The purified DNA samples were subjected to 10% denaturing polyacrylamide gel electrophoresis and were detected using the Typhoon FLA9500 (GE Healthcare) and analyzed with the ImageQuant TL software (GE Healthcare).
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bioRxiv - Genomics 2019Quote: 10 μg of total RNA were loaded on denaturing 1.2% agarose gel and transferred to Hybond™-XL nylon membrane (GE Healthcare). 32P-labelled oligonucleotides (see Table S4 ...
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bioRxiv - Zoology 2019Quote: ... DNA quality was assessed by running a subsample on a 1% agarose gel and the quantity of DNA was measured using a NanoVue (GE Healthcare). DNA libraries were constructed by Macrogen using a TruSeq DNA PCR-free library kit (350bp insert size) ...
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bioRxiv - Microbiology 2021Quote: ... Gels were exposed to auto radiographic film and visualized by a phosphor imager (Typhoon FLA-9000, GE Healthcare Life Sciences, USA). All the above reactions and separations were performed under anaerobic condition inside glove box (Plas-Labs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Between 3-20 µg of total protein per sample were resolved by SDS-PAGE (4-20% gels) and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked overnight at 4 °C in 5% milk or 5% BSA in PBST ...
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bioRxiv - Genomics 2021Quote: ... Gels were exposed on a phosphorimager screen overnight at -80°C and screens were scanned using a Typhoon scanner (GE healthcare). Images were analysed using the ImageQuant software (GE healthcare).
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bioRxiv - Biochemistry 2020Quote: P3 membranes were solubilized as described in the previous section and 200 μL of the detergent-solubilized supernatant was injected in a Superose 6 10/300 GL gel-filtration column (GE healthcare) equilibrated with 20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... and the digestion products were then loaded in 7 M urea containing 20% (wt/vol) polyacrylamide gels (acrylamide/bisacrylamide ratio 19:1) buffered with TBE and visualized using a Fluorescent Image Analyzer Typhoon (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions of protein were then concentrated and loaded onto a 10/300 Superdex200 Increase gel filtration column (GE Life Sciences) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was inactivated at 75 °C for 10 min and the labeled RNA/DNA was purified by gel filtration (Illustra MicroSpin G-25 columns; GE Healthcare). Recombinant ...