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Citations for GE Life Sciences :
1751 - 1800 of 1897 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the strip were separated by SDS-PAGE (200 V, 5 h) and the gel was imaged using a Typhoon 9500 fluorescence imager (GE Healthcare, Germany) at a resolution of 100 μm/pixel for Cy2 at 488 nm excitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μg of total RNA was resolved on a denaturing 1% agarose/1.25% formaldehyde gel using Tri/Tri buffer (Mansour and Pestov 2013) and transferred to a Hybond-XL membrane (GE Healthcare, RPN 303S). UV-crosslinked membranes were stained with methylene blue (0.025% w/v ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were run on the gel at 300V for 135 minutes using a gel transfer system (Doppel-Gelsystem Twin L, PerfectBlue).The samples were transferred to a Hybond-XL membrane (GE healthcare, RPN203S) at 50V for 1 hour at 4°C and then cross-linked using 0.12J (UV-lamp T8C ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Microbiology 2023Quote: ... A portion (20.9 mg) of this LPS preparation was further purified by gel permeation chromatography on Sephacryl S-400 HR (GE Healthcare, Uppsala, Sweden) on a column (2.5 × 120 cm ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Molecular Biology 2023Quote: ... The precipitate was then resuspended in BD buffer (BC buffer with 50mM Ammonium sulphate) with 500mM KCl and fractionated by molecular weight on the gel filtration column Sephacryl S-400 (GE healthcare, USA) with a fractionation range of 2×104–8×106 Da ...
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biochemistry 2023Quote: The changes in the oligomeric state of PrASNase were studied using the gel filtration column Superdex-200 (10/300 GL Increase, GE Healthcare, USA). The PrASNase was freshly purified and distributed equally in two different vials ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was subjected to further size-exclusion chromatography (SEC) by a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare, USA) using a flow rate of 0.3 mL/min and a running buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were run on 10% polyacrylamide SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran 0.45 µm NC - GE Healthcare Life Sciences). Membranes were blocked for 30 minutes in 5% milk in TBST buffer and incubated overnight with primary antibody (1:1000) ...
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bioRxiv - Microbiology 2023Quote: ... All genes used in the curves were purified with the IllustraTM GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) for better efficiency of the standard curves ...
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bioRxiv - Biochemistry 2022Quote: ... Purity and molecular weights were analyzed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analytical size exclusion chromatography (SEC) using a Superdex™ 75 5/150 GL column (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... All samples were loaded with the same amount of total protein (40μg) and separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (GE Healthcare, Buckinghamshire, UK) using a Transfer-Blot system (Bio-rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-3xFLAG-V1H(1-351) was further purified by gel filtration on a Superdex 200 10/300 GL column (GE Life Sciences) into the appropriate buffer for the subsequent application ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
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bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... and gel filtration chromatography on a HiLoad 16/60 Superdex 200 column controlled by an AKTA pure FPLC system (GE Life Sciences). The protein was concentrated to ∼3 mg/ml in buffer containing 150 mM NaCl ...
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bioRxiv - Evolutionary Biology 2023Quote: Native PAGE with Laemmli buffer (pH 8.8) without SDS was performed in 4–20% gradient polyacrylamide gels in a miniVE Electrophoresis System (GE Healthcare, Life Science). High molecular weight standards (Pharmacia ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Genetics 2023Quote: ... analysis and were transferred from gels to a nitrocellulose membrane (Cat#10600003, Amersham Protran Premium 0.45 μm NC; GE Healthcare Life sciences). Membranes were probed with rabbit polyclonal p44/42 antibodies (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured by Bradford assay and 20 µg of denatured protein (in Laemmli buffer at 95°C for 5 min) was loaded in a 7.5% polyacrylamide gel and blotted onto a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK). The membranes were blocked in 5% milk in PBS with 0.01% Tween (PBST ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through was then collected and concentrated using a 100 kDa concentrator and further purified on a Superose 6 gel filtration column (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... cell lysates corresponding to 2×105 cells in the case of in vivo experiments or 4×105 per sample in the case of nuclease-treated lysates were analyzed by electrophoresis on 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science). The proteins of interest were probed with antibodies against the following proteins ...
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bioRxiv - Genetics 2021Quote: ... All digested DNA was separated by electrophoresis on a 1% TBE agarose gel and subsequently transferred by capillary transfer to an Amersham Hybond-N+ nylon membrane (GE Healthcare, Milwaukee, WI).
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bioRxiv - Molecular Biology 2021Quote: ... The protein extracts were resolved by SDS-PAGE and the incorporation of 35S-Methionine was detected by autoradiography of the dried gel using film or a Phosphoimager (Typhoon Trio, GE Healthcare, Freiburg, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Biophysics 2019Quote: ... was used to remove the residual impurities by passing through a Superdex 200 10/300 GL gel filtration column (GE Healthcare Life Sciences). The peak fractions were collected and concentrated to 10-12 mg/ml ...
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bioRxiv - Biophysics 2019Quote: ... The fractions corresponding to pure DGRASP55/65 were then concentrated and applied into a Superdex200 10/300 GL gel filtration column (GE Healthcare Life Sciences) equilibrated in a 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Biophysics 2019Quote: ... The mAb TS2/4-biotin was purified from an excess of NHS-LC-LC-Biotin via gel filtration (Superdex-200 10/300; GE Healthcare Life Sciences), concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Genomics 2021Quote: ... DNA was stained with 0.2 µg/ml ethidium bromide (EtBr) (Nacalai Tesque, 14631-94) and detected using a Typhoon FLA9000 gel imaging scanner (GE Healthcare, Chicago, Illinois). Gel images were processed using ImageJ 1.8.0 (NIH ...
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bioRxiv - Cell Biology 2019Quote: ... Nanobodies were analyzed by SDS-gel electrophoresis and autoradiography using BAS Storage Phosphor Screens and a Typhoon FLA7000 IP phosphorimager (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag with the 3C protease (MERCK) ...
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bioRxiv - Biophysics 2020Quote: ... the AF555- or AS635P-conjugated and biotinylated H57-scFVs were purified by gel filtration using Superdex 75 (10/300 GL, GE Healthcare Life Sciences). Fractions containing monomeric ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Biophysics 2020Quote: ... the H57-scFV was concentrated with a stirred 10 kDa ultrafiltration cell (PBGC04310, MERCK and purified by means of gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) using an ÄKTA pure chromatography system (GE Healthcare Life Sciences) ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK ...
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bioRxiv - Biochemistry 2020Quote: ... the reaction mixture was applied to a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare Life Sciences #28-9909-44) to remove excess glutaryl-CoA/succinyl-CoA ...
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bioRxiv - Biochemistry 2020Quote: ... The products were separated by electrophoresis on a 15% polyacrylamide gel under denaturing conditions and subjected to autoradiography for quantitation using the Typhoon imaging system (GE Healthcare Life Sciences) and analysed in the Quantity One software (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... The products were separated by electrophoresis on a 10% polyacrylamide gel under denaturing conditions and subjected to autoradiography for quantitation on the Typhoon imaging system (GE Healthcare Life Sciences) and were analysed using Quantity One software (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Approximately 33 mg of CBL or 10 mg of mSLAP2 were loaded individually onto a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare Life Sciences) equilibrated with GF buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Peak fractions were pooled and further purified by gel filtration using a Superdex 200 Increase 10/300 GL (GE Healthcare, 28-9909-44) column equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: Samples for SAXS analysis performed in batch/static mode were prepared by additional purification over a Superdex S200 gel filtration column (GE Life Sciences Inc.) in 25 mM HEPES pH 7.5 ...