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Citations for GE Life Sciences :
1801 - 1850 of 1897 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and 25μg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... 20 μg RNA samples were separated on a denaturing 12% polyacrylamide gel and transferred to a membrane (Amersham™ Hybond™ -N+ Membrane, GE Healthcare). Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... Protein separation was carried out by SDS-PAGE in 10% bis-acrylamide gels and transferred to nitrocellulose membranes (Amersham Protran; pore size, 0.45 μm; GE Healthcare, Chicago, IL, USA). The membranes were blocked with 5% fat free milk in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were dried in a BIO-RAD Model 583 gel dryer and developed using the Typhoon FLA 9500 biomolecular imager (GE healthcare Life Sciences).
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bioRxiv - Molecular Biology 2022Quote: ... The RNA in the gel was transferred overnight to a nitrocellulose membrane with a positive charge (GE healthcare Amersham™ Hybond™ -N+) by capillary transfer using an assembly of Whatman® filter paper ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was exposed with phosphor imaging screens for 5 minutes before being scanned with a Typhoon™ FLA9500 (GE Healthcare Life Sciences). The bands corresponding to the probes were cut out of the gel and conserved at -20°C for future work ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μg total RNA from Col and ktn1-20 was resolved on 15% Urea-PAGE gels and transferred onto Hybond NX membranes (GE Healthcare Life Sciences) followed by chemical cross-linking using EDC (1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: Protein samples were separated by standard Laemli SDS-PAGE on 9% Tris-Glycine gels and transferred onto nitrocellulose membrane (GE Healthcare Bio-Sciences). PhosTag gels were prepared by adding 200 μM PhosTag™ (WAKO chemicals ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was visualized using Typhoon laser scanner and Image Quant TL software (normal sensitivity and PMT 380 V; GE Healthcare Life Sciences). The low range ssRNA ladder (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... OTUB1 and its catalytic mutant (C91S) were further purified by gel filtration chromatography on a HiLoad 16/60 Superdex 75 (GE Healthcare Life Sciences) column equilibrated in 25 mM HEPES pH 7.6 ...
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bioRxiv - Microbiology 2019Quote: ... Fluorescent detection and images of the gels and were taken using a Typhoon FLA 9000 imager and the requisite software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2019Quote: ... The gel was again washed and exposed as above and the image was acquired using Amersham™ Typhoon™ Biomolecular imager (GE Healthcare). The images were quantified using Aida Image Analysis software ...
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bioRxiv - Biochemistry 2019Quote: ... results were confirmed by SDS-PAGE of samples from the reaction mixtures and fluorescence imaging of the gel with a Typhoon FLA 9500 laser scanner (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2020Quote: ... The Vip3Aa200-end proteins were further purified by HiTrap Q HP ion-exchange chromatography and Superdex 200 gel filtration chromatography (GE Healthcare Life Sciences). Fractions containing the Vip3Aa200-end protein were concentrated to ∼ 7 mg/ml for crystallization ...
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bioRxiv - Physiology 2021Quote: ... on an ÄKTA Pure Protein Purification System equipped with a Superdex 75 10/300 GL gel-filtration column and a 100 µL loop (GE Healthcare Life Sciences). Buffer was 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... and same protein quantities were separated on a 10% SDS-PAGE gel under reducing conditions and transferred to 0.45 μm PVDF blotting membrane (GE healthcare, Chicago, IL, USA). Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... AF488-labeled samples were separated by SDS-PAGE and fluorescence in gels was captured with an Amersham Imager 680 (GE Healthcare Life Sciences). To ensure that equal amounts of protein were loaded in each lane ...
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bioRxiv - Immunology 2021Quote: ... The elimination of imidazole was achieved by gel filtration on columns filled with 10 ml of Sephadex G-25 (GE Healthcare Life Sciences) previously equilibrated with ice-cold PBS ...
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bioRxiv - Plant Biology 2019Quote: We extracted RNA according to Oliveira et al.33 and evaluated its integrity and purity using agarose gel electrophoresis and a NanoVue spectrophotometer (GE Healthcare Life Sciences). lllumina TruSeq RNA Sample Preparation kits (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Quantification of band intensities was carried out on digital images of membrane samples provided by LAS4000 gel documentation system (GE Healthcare; Barcelona, Spain) using ImageJ software (1.50b ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of prepared protein samples were loaded and separated on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare, Buckinghamshire, UK). NZYColour Protein Marker II (NZYTech ...
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were further purified via size exclusion chromatography using a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare, Chicago, IL) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitated proteins were analyzed by SDS-PAGE followed by phosphorimaging of dried gels using a Molecular Dynamics Typhoon system with native ImageQuant Software (GE Healthcare, Piscataway, NJ).
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bioRxiv - Cell Biology 2021Quote: ... and 25µg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... The gel was then dried and exposed on a phosphor screen overnight before being visualized on a Typhoon FLA9500 (GE Healthcare Life Sciences).
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bioRxiv - Bioengineering 2022Quote: ... The 100 mM and 250 mM fractions containing the eluted endolysins were dialyzed against PBS at 4 °C and they were further purified using gel filtration (fast protein liquid chromatography) on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The final step was LPS removal ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant containing the cleaved protein was separated from the resin by centrifugation and loaded on to HiLoad 16/600 Superdex 75 gel filtration column (GE Healthcare Life Science) equilibrated with 20mM Bis–Tris (pH 6.5) ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μg total protein for each condition was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences) at 350 mA for 3 h for blotting ...
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bioRxiv - Physiology 2022Quote: ... and ran this mixture through handcast Tris-glycine gels prior to blotting onto nitrocellulose membranes with 0.1 µm pore size (GE Healthcare, Chicago, IL, US). Subsequently ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was visualized using Typhoon laser scanner and Image Quant TL software (normal sensitivity and PMT 380 V, GE Healthcare Life Sciences). The low range ssRNA ladder (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was visualized using Typhoon laser scanner and Image Quant TL software (normal sensitivity and PMT 380 V, GE Healthcare Life Sciences). The low range ssRNA ladder (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty micrograms of total RNAs were separated on 15% acrylamide/8 M urea gel and transferred onto an Amersham™ Hybond™-N+ nylon membrane (GE Healthcare). The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were further purified by HiTrap Q HP ion-exchange chromatography and Superdex 200 Increase 10/300 GL gel filtration chromatography using an ÄKTApure chromatography system (GE Healthcare Life Sciences). Proteins corresponding to the molecular weight of the tetramerized Vip3Aa were used for subsequent biochemical and bioassay analyses ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein from this affinity step was concentrated and further purified by gel filtration on a HiLoad® 16/600 Superdex® 200 pg column (GE Healthcare) equilibrated in 20 mM TrisHCl ...
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bioRxiv - Biophysics 2023Quote: ... The digested protein was concentrated using a 100 kDa concentrator and further purified on a Superose 6 Increase 10/300 gel filtration column (GE Healthcare Life Sciences) equilibrated in an Mg2+-containing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). Rabbit polyclonal anti-SDHC (1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then incubated for 30 minutes at room temperature and excess copper was removed by gel filtration using a PD MidiTrap G-25 desalting column (GE Healthcare; Uppsala, Sweden) equilibrated with acetate buffer (25 mM sodium-acetate ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant A3A-MycHis was expressed and purified as described previously to provide a positive control for deamination activity.49 Samples were run on a 15% TBE-Urea gel and imaged using a Typhoon 7000 FLA biomolecular imager (GE Healthcare Life Sciences).
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bioRxiv - Plant Biology 2023Quote: ... and their fluorescence was recorded by direct scanning of the gel using an image analyser (Typhoon 9600, GE Healthcare Bio-Science, Chicago, IL) as described (Tasaki et al ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Biochemistry 2023Quote: The oligosaccharide products were purified by semi-preparative gel permeation chromatography using three HiLoad® 26/600 Superdex® 30 pg (GE Healthcare) columns mounted in series and connected to a semi-preparative size-exclusion chromatography system which consisted of a Knauer pump (pump model 100) ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Purified full-length and MTases underwent an extra step of size exclusion chromatography on a gel filtration column (HiLoad® 16/600 Superdex® 200 pg, GE Healthcare) against 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... Germany) and gel filtration fast protein liquid chromatography (FPLC) was performed on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The sample was dialyzed against phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Unencapsulated ANTS and DPX were removed by gel filtration through a 5 mL HiTrap Desalting Sephadex G-25 resin column (GE Healthcare Life Sciences). To ensure the correct size and obtain LUVs ...
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bioRxiv - Microbiology 2024Quote: ... The His-tag S protein was purified by gel filtration using the Akta explorer 100 system with a high-load Superdex pg 200 16/600 column (GE Life Sciences, USA). Main peak fraction was collected ...