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Citations for GE Life Sciences :
1501 - 1550 of 1897 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Individual reactions were transferred to a 96-well plate and analysed using a Superdex 200 Increase 5/150 GL gel filtration column (GE Healthcare) attached to an Agilent 1200 Series Gradient HPLC system ...
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bioRxiv - Cell Biology 2022Quote: ... then the protein was recovered in the flow-through and separated from thrombin and last contaminants using gel filtration (Superdex 200 pg HiLoad 16/600 column, GE healthcare). The final yield was typically 20 mg (LB ...
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bioRxiv - Biophysics 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin and gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Biochemistry 2022Quote: ... coli was essentially performed as described 15 with the modification that 25 mM HEPES buffer was used as buffer component in all purification steps and the final gel filtration was performed on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cleaned with an Illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Chicago, IL, USA) and sequenced by using Sanger technology in an ABI 3730XL sequencer (STAB-VIDA ...
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bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 10 ml Strep-Tactin column (Iba) and a gel filtration column (Superdex200 16 600, GE Healthcare). The purified protein was stored in PBS containing 1 mM TCEP at -80°C ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples were run on 5∼20% precast gradient SDS-PAGE (ATTO) or 4∼20% precast gradient gel (Shanghai WSHT Biotechnology Inc.) and then transferred to Hybond ECL membranes (GE Healthcare) followed by western blot analysis with the indicated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) and collected in gel filtration buffer (20mM PIPES pH6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) and collected in gel filtration buffer (20mM PIPES pH6.8 ...
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bioRxiv - Biophysics 2022Quote: ... and then purified from excess salt and potential oligomeric species using gel filtration on a size exclusion column (Superdex 75 10/300 GL, GE Healthcare) at a flow rate of 0.5 mL/min and eluted in 20 mM sodium phosphate 0.2 mM EDTA buffer (at pH 8.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the resultant [18F]RL-III-5F7 conjugate was isolated by gel filtration over a PD10 column (GE Healthcare, Piscataway, NJ) eluted with PBS ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing PomXN_K13AR15A-His6 were pooled and concentrated with an Amicon Ultra-15 centrifugation filter device with a cutoff of 3kDa and loaded onto a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare) that was equilibrated with dialysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Lysate equivalent of approximately 2×105 cells per well were run on NuPage 4-12% Bis-Tris Gel (Novex) and transferred to Amersham Hybond-P PVDF membranes (GE Healthcare). Membranes were blocked with 2% nonfat dry milk (Lab Scientific) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated on 10 % acrylamide gels by SDS-PAGE and protein transferred to a 0.45 μm PVDF membrane (GE Healthcare 15259894) using the BioRad TransBlot Turbo system ...
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bioRxiv - Microbiology 2021Quote: ... and 633 nm (Cy5) nm and finally verified by SDS-PAGE by measuring in-gel fluorescence with LAS4000 Imagequant (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and further purified with size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) in a buffer composed of 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... and the elution was concentrated to 1mL and loaded onto the size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated with 50 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction products were resolved on 6–8% (w/v) denaturing polyacrylamide gels and visualized with Typhoon FLA scanner (GE Healthcare). In total ...
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bioRxiv - Molecular Biology 2019Quote: ... RNAP was then mixed with an excess of σ70 and loaded onto a 16/60 Superdex 200 prep grade gel filtration column (GE Healthcare) with buffer A (20 mM TRIS pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA complexes were incubated at 25 °C for 15 mins before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). The peak corresponding to DNA-protein complex was collected and concentrated ...
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bioRxiv - Microbiology 2021Quote: ... lysed by sonication and purified using nickel affinity chromatography followed by gel filtration with either a Superdex 75 (NT1, NT2, RgpB-CTD) or 200 (rSslE, NT1-NT2, NT3-M60) column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare). The purified antibodies were either used in binding and neutralizing assays.
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bioRxiv - Cell Biology 2021Quote: ... The concentrated BASS-XL tetramer was applied on a HiLoad 16/600 Superdex 200 gel filtration column using an AKTA Purifier (GE Healthcare). Crystallization conditions were screened using a Mosquito Robot (TTP Labtech ...
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bioRxiv - Microbiology 2020Quote: ... concentrated using a Vivaspin 20 centrifugal concentrator (10-kDa molecular weight cutoff) and applied to a HiLoad 16/600 Superdex 75 pg gel filtration column (GE Healthcare). Pure protein samples were dialyzed against 10 mM TrisHCl pH 7.0 and concentrated as described above ...
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bioRxiv - Plant Biology 2021Quote: ... The chloroform extracts were loaded on lanes of LK6D Silica gel 60A TLC plates (GE healthcare Japan Co. Ltd, Tokyo, Japan), and the plates were put in a chamber with hexane ...
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bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified by gel filtration chromatography on a HiLoad™ 16/600 Superdex 200 pg column (GE Healthcare) with the following buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was analyzed by electrophoresis on a denaturing 8% polyacrylamide gel containing 8 M urea followed by transfer to a positively-charge Hybond nylon membrane (GE Healthcare). Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol ...
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bioRxiv - Microbiology 2021Quote: ... A constant amount of total RNA (between 3.5 and 14 μg) was loaded on 8% urea acrylamide gel in 1X TBE and transferred to a nitrocellulose membrane (GE Healthcare). For detection ...
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bioRxiv - Microbiology 2021Quote: ... Gels were dried and exposed on a phosphoscreen for 2-4 days and visualized using a Typhoon Imaging System (GE Healthcare). Bands were quantified using band densitometry using the ImageQuant software (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
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bioRxiv - Biochemistry 2020Quote: ... The intensity of the gel bands corresponding to the substrate and the product were quantitated with Typhoon TRIO Phosphorimager (GE Healthcare) using ImageQuant TL software (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were resolved on 10-14 % polyacrylamide gels containing 7 M urea and analyzed with a Typhoon TRIO Phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... His-Thrombin-TEV-Nsp15 variants were eluted with 250 mM imidazole and further purified by gel filtration on a Superdex-200 column (GE Healthcare) in SEC Buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... protein extracts were resolved on SDS-PAGE gels under denaturing conditions and transferred to Hybond ECL membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1 hour at RT and incubated overnight with the respective primary antibody (Supplementary Table S2 ...
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bioRxiv - Immunology 2021Quote: ... The peak fractions were collected and purified by size exclusion chromatography using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Peak protein fractions were pooled and loaded onto the size-exclusion chromatography column using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were further purified by gel filtration chromatography using a Superdex 200 column attached to an AKTA-format FPLC (GE Healthcare) and running buffer containing 50mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... CtNaa5082-289 was subsequently purified by SEC (size-exclusion chromatography) using a Superdex 75 26/60 gel filtration column (GE Healthcare) in buffer G500 (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2022Quote: Recombinant pGEM-T easy vectors used to sequence the lncov1 full-length were digested with EcoRI (Fermentas) and purified again with the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). In vitro reverse transcription was performed using the RiboMax T7 system (Promega ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Immunology 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin followed by gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed and His-ALOD4 and His-OlyA were isolated by nickel purification followed by size exclusion chromatography (HisTrap-HP Ni column, Tricorn 10/300 Superdex 200 gel filtration column; FPLC AKTA, GE Healthcare). Protein-rich fractions were pooled and concentration was measured using a NanoDrop instrument.