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Citations for GE Life Sciences :
1651 - 1700 of 4701 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved GST-tag and bZip construct were separated by gel filtration chromatography using Hi-Load 16/600 Superdex 200 pg (GE Healthcare) on AKTA pure in a 20 mM MES pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... The cleaved AVR proteins were then eluted and further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare). Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... The SAFB2 SAP and RRM domains were further purified via size exclusion on a HiLoad 16/600 SD 75 (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Size Exclusion Chromatography (SEC) was performed using a Superdex 200 16/70 or Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: Coupled PEG-PhyB and PEG-PIF6 were purified by size exclusion chromatography using a HiLoad Superdex 200 pg (16/600 or 25/600, GE Healthcare) column connected to an ÄKTA Explorer system with PBS as a running buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... Complete cleavage by 3C was visualised by Coomassie-stained SDS-PAGE and the cleaved sample was purified further by size exclusion chromatography (SEC) on a Superdex 75 16/600 column (GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was further purified using ion exchange chromatography preceding size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) into CRY buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The fractions with high purity were concentrated using 10 kDa cutoff Amicon filters and then further purified by FPLC using a HiLoadTM 16/600 SuperdexTM 200 pg column (GE Healthcare) equilibrated with sizing buffer (500 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... with a 10 kDa cut-off and loaded onto a Superdex 200 pg HiLoad 16/600 (GE Healthcare, 1 mL/min). Sample purity was assessed by 12% SDS-PAGE analysis and sample concentration was determined by spectrophotometry using a theoretical molar extinction coefficient at 280 nm of 20 ...
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bioRxiv - Molecular Biology 2023Quote: ... aureus EF-G was purified further by gel filtration on a Hiload 16/60 Superdex-200 column (GE Healthcare, Uppsala, Sweden) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved BRCA2 Ex14 was further purified by HiTrapSP HP ion exchange chromatography and size exclusion chromatography (HiLoadTM 16/600 Superdex 200, GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
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bioRxiv - Biochemistry 2023Quote: ... Desired fractions were incubated with 0.5 mM FeCl3 in presence of 200 mM NaHCO3 for 1h at room temperature and further purified SEC on a S200 16/600 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated Hsp10 preps were first diluted tenfold with chromatography buffer before loading into a HiPrep SP XL 16/10 column (GE Healthcare). A sodium chloride linear gradient from 0 to 1 M was used to purify Hsp10 and it eluted at a conductivity of 13.2 mS/cm ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved DMC1 was further purified by HiTrapQ HP ion exchange chromatography and size exclusion chromatography (HiLoadTM 16/600 Superdex 200, GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... with a 10 kDa MWCO before being subjected to size exclusion chromatography (SEC) on a Superdex 200 16/60 column (GE Healthcare) connected to an Äkta FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... LmdC was further purified by size exclusion chromatography (SEC) on a HighLoad 16/60 Superdex 200 pg column (GE Healthcare, USA) equilibrated with SEC buffer (20 mM Tris/HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... stored in sample loops on the system and injected into a Gel Filtration column (HiLoad 16/60 Superdex 200 prep grade, GE Healthcare). The protein sample was concentrated using Vivaspin 20 filter concentrators (VivaScience ...
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bioRxiv - Cell Biology 2024Quote: ... We pooled the α-syn-containing fractions and further purified the protein by size-exclusion chromatography on a HiPrep Sephacryl S-200 HR 16/60 gel filtration column (GE Healthcare) in 50 mM NH4Ac pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... Concentrated proteins were further purified by Size-exclusion chromatography (SEC) either using a HiLoad 16/60 Superdex 200 pg column (GE Healthcare) or a HiLoad 16/60 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... This elution was subjected to a second polishing step via size-exclusion chromatography using a HiLoad Superdex S200 16/600 column (GE Healthcare) in the presence of 1x TBS pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
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bioRxiv - Immunology 2024Quote: ... A further size exclusion chromatography (SEC) polishing step was performed on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was diluted to half with 40 mM BisTris pH 6.8 and loaded onto HiPrep™ Q FF 16/10 anion exchange chromatography column (GE healthcare), washed with 20 mM BisTris pH 6.8 - 100 mM NaCl.
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bioRxiv - Neuroscience 2023Quote: ... The cleaved protein was then separated from tag and TEV by a second Ni-NTA affinity column and further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) in 50 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... These eluted proteins were subsequently passed through a superdex s75 16/60 column attached to an AKTA Pure system (GE healthcare) in 50 mM Tris pH 8.0 and 300 mM NaCl.
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bioRxiv - Molecular Biology 2024Quote: ... All proteins underwent an additional purification step by size exclusion chromatography (SEC) on a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare) in SEC Buffer (50 mM Na2HPO4 ...
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bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... A final size exclusion chromatography (SEC) step was performed using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare), with running buffer 20mM HEPES 7.4 ...
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bioRxiv - Biochemistry 2024Quote: A second purification step on Ni-NTA beads was performed to remove any uncleaved protein before to achieve a SEC purification onto a Superdex 75 16/600 GE column (GE Healthcare) in a final buffer containing 10 mM Tris pH = 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... The SEC was performed as the final purification step using HiLoad® 16/600 Superdex 200 or Superdex 75 (GE Healthcare) column pre-equilibrated with buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions containing the protein complexes were pooled and further purified by size exclusion chromatography using a 16/60 Superdex 200 column (GE Healthcare) equilibrated with a buffer containing 200 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... SpyTag003-linked non-antigen binding proteins) were injected onto a pre-equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare), or a HiLoad 16/600 Superdex 75 pg column (SUMO-SpyTag003 and affibodies ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was separated on a Ni Sepharose 6 Fast Flow (GE Healthcare) column using 30 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... hTRPC5 protein was further purified by size exclusion chromatography (Superose-6, GE Healthcare) in buffer containing TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... which was further purified using a Superose 6 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... and then loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified with Ni-sepharose fast flow 6 resin (GE Healthcare, Boston, USA) by the elution with 250 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the size-exclusion chromatography (Superose 6 10/300 GL, GE Healthcare) in buffer containing 20 mM MOPS-Na ...
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bioRxiv - Molecular Biology 2021Quote: ... and was injected into a Superose 6 Increase 10/300 column (GE Healthcare) in buffer H200 (20 mM Hepes ...
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bioRxiv - Molecular Biology 2019Quote: ... and fluorescent size-exclusion chromatography (FSEC) (Superpose-6 10/300 GL, GE Healthcare) using buffer C (50mM Na-PIPES ...
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bioRxiv - Biochemistry 2019Quote: ... and further purified on a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer C (20 mM Tris pH8 ...
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bioRxiv - Neuroscience 2019Quote: ... Odor was added to a 6 mm filter disc (GE Healthcare #2017-006) placed on one end of the petri-dish and the diluent ...
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bioRxiv - Biophysics 2019Quote: ... and subjected to size-exclusion chromatography using a Superose 6 column (GE Healthcare) with Wash Buffer containing 0.006% DMNG ...
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bioRxiv - Immunology 2019Quote: ... 94°C 5min) with 6 µl of RNA and random hexamers (GE Healthcare). A PCR was then performed based on an established protocol (Tiller et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The clear supernatant was incubated with Ni-Sepharose 6 Fast Flow (GE Healthcare) to capture the His-tagged protein ...