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Citations for GE Life Sciences :
1901 - 1950 of 4701 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Cell Biology 2020Quote: ... we used a DeltaVision|OMX microscope version 3 (GE Healthcare) with an objective lens 100X UPlanSApo NA1.40 Oil (Olympus ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~7 mg/mL ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Immunology 2020Quote: ... and protein A-Sepharose (PAS, 3 mg/sample, GE Healthcare), after a preclearing step on PAS (1 h ...
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bioRxiv - Cell Biology 2020Quote: ... were used first for an affinity-based batch purification process which was followed by size exclusion chromatography using an ÄKTApurifier system and a High Load 16/60 Superdex 200 SEC column (both GE Healthcare Life Sciences). Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and then applied to an initial size-exclusion chromatography (SEC) step using HiLoad 16/60 Superdex 200 (GE Healthcare, Chicago IL) in SEC200 buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... expressed aSyn was purified using ion exchange chromatography (IEX) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub ...
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bioRxiv - Molecular Biology 2020Quote: ... The collected eluate was concentrated to 2.6 mg/ml using a Sartorius VivaSpin20 column (10 K MW cut-off) for additional purification by Size-Exclusion Chromatography (SEC) using a HiLoad® 16/600 Superdex® 75 prep grade column (GE Healthcare) in Gel Filtration Buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Binb SH3 sample was immediately loaded on size exclusion chromatography column HiLoad™ 16/600 Superdex™ 75 prep grade column (GE Healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 2 mL in a 30 kDa MWCO spin concentrator and applied onto a HiLoad 16/600 Superdex 200 pg size exclusion chromatography (SEC) column (GE healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were further purified according to their size on a HiLoad™ 16/60 Superdex™ 200 prep grade prepacked column (GE Healthcare) using an ÄKTAprime chromatography system at 4°C with a SEC buffer containing 50 mM Tris/HCl ...
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bioRxiv - Biochemistry 2021Quote: ... AMDHD2 was further purified according to its size on a HiLoad™ 16/60 Superdex™ 200 prep grade prepacked column (GE Healthcare) using an ÄKTAprime chromatography system at 4°C with a SEC buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were concentrated and loaded on to a HiLoad 16/ 600 Superdex 75 pg column (GE Healthcare Life Sciences, Piscataway, NJ, USA). For biophysical measurements ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified by nickel-affinity chromatography and samples were passed over a HiLoad® 16/600 Superdex® 75 or 200 pg column (GE Healthcare), equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length GFAT-1 and the isomerase domain were further purified according to their size on a HiLoad™ 16/60 Superdex™ 200 prep grade prepacked column (GE Healthcare) using an ÄKTAprime chromatography system at 4°C with a SEC buffer containing 50 mM Tris/HCl ...
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bioRxiv - Immunology 2021Quote: ... Samples were eluted from the corresponding immunoaffinity matrix with 3M MgCl2 buffer and subjected to size-exclusion chromatography (SEC) on a HiLoad® 16/600 Superdex® pg200 (GE Healthcare). Samples were subsequently concentrated to 1 mg/ml and stored frozen in DPBS (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Eluted particles were concentrated using an Amicon Ultra 15 mL 30K concentrator (MilliporeSigma) and purified by SEC using a HiLoad® 16/600 Superdex® 200 (GE Healthcare) column equilibrated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The protein purity and enrichment were confirmed by SDS-PAGE and size exclusion using a HiLoad® 16/600 Superdex® 200 pg column using an ÄKTA pure (GE healthcare). However ...
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bioRxiv - Immunology 2020Quote: ... and purified with PGT145 affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) as described previously 53.
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bioRxiv - Biochemistry 2020Quote: ... These affinity-purified protein concentrates were then applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences) packed with dextran covalently bound to highly cross-linked agarose ...
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bioRxiv - Microbiology 2019Quote: ... and then further purified by FPLC size exclusion chromatography using a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare Life Sciences). Pure 6xHis+t-ShyA fractions were collected and concentrated up to 11.4 mg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... OTUB1 and its catalytic mutant (C91S) were further purified by gel filtration chromatography on a HiLoad 16/60 Superdex 75 (GE Healthcare Life Sciences) column equilibrated in 25 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing TcB-TcC were subjected to SEC using a Superdex 200 10-300 or a Superdex 200 16-60 column (GE Healthcare Life Sciences) equilibrated in gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The lysate was then subjected to nickel-immobilized metal affinity chromatography (IMAC) using a HisPrep™ Fast Flow 16/10 column (GE Healthcare, USA) equilibrated with equilibration buffer (20 mM NaH2PO4 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The precipitated protein was resuspended in 100 mM HEPES buffer (pH 7.4) and subjected to SEC using a HiPrep™ 16/60 Sephacryl® S-400 column (GE Healthcare, USA) using 100 mM HEPES Buffer ...
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bioRxiv - Immunology 2020Quote: ... and BG505 SOSIP.v5.2 were expressed in HEK293F cells and purified with either PGT145 or 2G12 affinity chromatograph followed by size exclusion chromatography (SEC) using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) as described previously 7 ...
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bioRxiv - Immunology 2020Quote: ... Eluted VLPs were concentrated using an Amicon Ultra 15 mL 30K concentrator (MilliporeSigma) and further purified by SEC using a HiLoad® 16/600 Superdex® 200 (GE Healthcare) column equilibrated with 500 mM glycine pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Eluted NPs were concentrated using an Amicon Ultra 15 mL 30K concentrator (MilliporeSigma) and further purified by SEC using a HiLoad® 16/600 Superdex® 200 (GE Healthcare) column equilibrated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... concentration to 1-2 mL in 10 kDa MWCO spin concentrators and applied to either a HiLoad Superdex 75 pg 16/600 or a Superdex 75 Increase 10/300 GL SEC column (GE Healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... αSyn-GLuc1 and αSyn-GLuc2 were polished by HiLoad 16/60 Superdex 75 size exclusion chromatography (SEC) (GE Healthcare Lifesciences, Marlborough, MA, USA), collecting fractions eluting at 47-61ml ...
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bioRxiv - Biophysics 2020Quote: ... The fractions with the protein were pooled and further purified by size exclusion chromatography using a 16/60 superdex 75pg column (GE Healthcare Life Sciences) equilibrated with a buffer containing 10 mM Tris pH 7.8 ...
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bioRxiv - Biophysics 2021Quote: ... was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, USA), equilibrated in storage buffer (50 mM HEPES pH 7.25 ...
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were further purified via size exclusion chromatography using a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare, Chicago, IL) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... affinity-purified proteins were further purified by size-exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences) in 1x TBS (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant containing the cleaved protein was separated from the resin by centrifugation and loaded on to HiLoad 16/600 Superdex 75 gel filtration column (GE Healthcare Life Science) equilibrated with 20mM Bis–Tris (pH 6.5) ...
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bioRxiv - Microbiology 2022Quote: ... Eluted particles were concentrated using an Amicon Ultra 15 mL 30K concentrator (MilliporeSigma) and SEC purified using a HiLoad® 16/600 Superdex® 200 (GE Healthcare) column equilibrated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Samples then underwent size exclusion chromatography in an ÄKTA Pure fast protein liquid chromatography (FPLC) using HiLoad 16/600 Superdex 200 or Superdex 75 column (GE Healthcare Life Sciences) in GF-300 buffer (25 mM HEPES buffer at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein from this affinity step was concentrated and further purified by gel filtration on a HiLoad® 16/600 Superdex® 200 pg column (GE Healthcare) equilibrated in 20 mM TrisHCl ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated and further purified by size-exclusion chromatography in purification buffer including 10 mM imidazole using the column HiLoad® 16/600 Superdex® 75 prep grade (GE healthcare). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Purified full-length and MTases underwent an extra step of size exclusion chromatography on a gel filtration column (HiLoad® 16/600 Superdex® 200 pg, GE Healthcare) against 20 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... After overnight cleavage with Ulp1 the His6-SUMO tag was removed by subsequent HisTrap purification and protein solution was subjected to size-exclusion by using HiLoad 16/60 Superdex 30 (for integrin constructs) or Superdex 75 column (for paxillin constructs, GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... TLC plates were visualized via exposure to a phosphor screen for 16 h prior to imaging using a phosphor screen and Typhoon FLA7000 (GE Healthcare, Pittsburgh PA). Silica gel plates used to assay the metabolism samples and lesion extracts were 150 Å Silica Gel HL 250µ m 10 x 20 cm channeled plates with a pre-adsorbent zone purchased from Analtech/iChromatography (Newark ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Microbiology 2024Quote: ... The His-tag S protein was purified by gel filtration using the Akta explorer 100 system with a high-load Superdex pg 200 16/600 column (GE Life Sciences, USA). Main peak fraction was collected ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... or 5-mL HisTrap column (GE Healthcare), respectively ...