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Citations for GE Life Sciences :
1451 - 1500 of 4823 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30-60 min at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C and 120 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare). For the experiments presented in Figure S2A and S2B another DeltaVision microscope equipped with a CoolSNAP-HQ2 cooled CCD camera (Photometrics ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Obtained gels and membranes were analyzed in Image Master 2D Platinum 7 Software (GE Healthcare, Chicago, IL, USA) to look for qualitative and/or quantitative differences in abundance/antigenicity of some venom proteins between the samples ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was diluted to 500 µl with running buffer A (50 mM Tris, pH 9) and loaded on a 1 ml Resource™ Q anion exchange column (GE Healthcare Life Sciences). A linear gradient to 40% running buffer B (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...
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bioRxiv - Biophysics 2021Quote: ... followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare). Purified Nap1 was snap frozen in buffer containing 10 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and concentrated by ultrafiltration (Amicon, 10 kDa cutoff,) before further purifying on a Superdex S75 16/60 gel filtration column (GE Healthcare) in 25 mM NaH2PO4/Na2HPO4 pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... The complex was supplied with 2mM GTP and incubated at 4°C for 1 hour prior to purification by size exclusion chromatography on a HiLoad Superdex S75 16/600 column (GE Healthcare) equilibrated in 50mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Remaining contaminants were removed using size exclusion chromatography on a HiLoad 16/600 Superdex 200 Column (GE Life Sciences, Marlborough, MA). Proteins that did not require fluorescent labeling were buffer exchanged using Amicon concentrators (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: ... ParB-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been equilibrated with 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Further purification was carried out by size exclusion chromatography using a Superdex™ 200 Hi Load 16/60 column (GE Healthcare). Proteins were eluted with SEC buffer (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... the affinity tag was removed from complexes collected after the nickel elution by an overnight TEV digestion against lysis buffer followed by size-exclusion chromatography using a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... ExoU was purified by an initial affinity step (immobilised nickel affinity chromatography) followed by size-exclusion chromatography (SEC) (16/600 Superdex 200, GE healthcare) in 20 mM Tris–HCl pH 8.2 ...
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bioRxiv - Molecular Biology 2020Quote: Purified Affimer proteins were incubated with KRAS lysates overnight at 4°C and the complexes purified by Ni-NTA affinity chromatography and size exclusion chromatography using HiPrep 16/60 Sephacryl S-100 column (GE Healthcare). The complexes were concentrated to 24 mg/ml (KRAS:K3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Pum-HD was then separated from the tag by reverse affinity chromatography and in the final step Pum-HD was further purified by size-exclusion chromatography (SEC) using HiLoad 16/600 Superdex 75 column (GE Healthcare) in 50 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixture was then concentrated by reducing the volume from 48 ml to 1 ml using a 3 kDa cutoff concentrator and the hb complex was purified by SEC using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare). The identity of the hb complex peak was confirmed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was passed through a HiLoad 16/600 Superdex 200 PG size exclusion column using an ÄKTA pure purification system (GE Healthcare). Peak fractions were pooled and concentrated using Amicon Ultra-4 centrifugal filter units (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the proteins were concentrated with Amicon Ultra 10K filter (viMillipore) and applied to HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions containing either AsCas12a or SpCas9 were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the heparin-purified protein was concentrated by ultrafiltration and applied to a size exclusion column (Superdex 200 16/600 column, GE Healthcare). The protein was eluted with Buffer C (20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was eluted by using purification buffer including 200 mM imidazole and further purified by gel chromatography on an ÄKTA pure system equipped with a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) running with purification buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing Mtb UgpB were pooled and loaded onto a Supderdex 75 pg HiLoad 16/600 gel filtration column (GE Healthcare) and eluted with buffer C (25 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: Peak fractions from the IEX were concentrated to < 4 ml and loaded onto a Superdex 16/60 gel filtration column (GE Healthcare) in Buffer A ...
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... before heating at 95°C for 5 min and samples were separated by SDS-PAGE using a 4-16% acrylamide gradient gel and blotted onto nitrocellulose membranes (Hybond-C Extra, Amersham Bioscience or Optitran BA-S 85, GE Healthcare). The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were further purified by size-exclusion chromatography (on either a Superdex 200 increase 10/300 GL or a HiLoad 16/600 Superdex 200pg column, both GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biochemistry 2019Quote: ... the sample was concentrated and then purified by size exclusion chromatography on a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The Biotin-labelled-Avi-His6-SUMO proteins were purified using a nickel-NTA column followed by gel-filtration chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare). Mouse and human TBK1 (residues 1 to 657 ...
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bioRxiv - Microbiology 2020Quote: ... The final purification step for all variants encompassed a size exclusion chromatography run (HiLoad 16/60 or 26/60 Superdex 75 or 200 prepgrade, GE Healthcare) using a buffer with 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... and the protein was further purified by size exclusion chromatography using a Superdex 75 16 600 column (GE Healthcare, Freiburg, Germany) and a 20 mM Tris-HCl pH 7.5 buffer.
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bioRxiv - Biophysics 2019Quote: ... The eluted BBSome:ARL6 complex was concentrated to 500 μl using a concentrator with a 100 kDa molecular weight cutoff (Thermo Fischer Scientific) and injected onto a Superdex 200 (16/600) size-exclusion chromatography column (GE Healthcare) equilibrated with 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... We purchased all columns (26/10 HiPrep [desalting], HisTrap HP [Ni], and HiPrep Q HP 16/10 [anion exchange]) from GE Healthcare, Inc ...
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bioRxiv - Developmental Biology 2019Quote: ... Size-exclusion chromatography was performed for the final purification of wild-type and mutant dP75 PWWP using Superdex G75 Hiload 16/60 column (GE Healthcare). Fractions containing target proteins were pooled and concentrated to 10 mg/ml in a buffer containing 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequently purified further by size-exclusion chromatography (SEC) using either a HiLoad 16/600 or HiLoad 26/600 Superdex 75 pg column (GE Healthcare) equilibrated with a buffer containing 10 mM HEPES pH 8.0 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... during His6-tag cleavage to remove phosphate groups resulting in inhibitory autophosphorylation ([19]. Size exclusion chromatography was carried out using a Superdex 200 16/600 column (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were further purified by size exclusion chromatography (SEC) using the HiLoad Superdex 16/600 200 prep grade column (GE Healthcare) run in 20 mM Tris-HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... The affinity chromatography fraction containing the antigen (as identified by SDS-PAGE) was concentrated and loaded onto a HiLoad 16/600 GL Superdex 75 column (GE Healthcare) pre-equilibrated in endotoxin-free PBS using an AKTA chromatography system (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant obtained at this step was filtered through 0.45 μm filters and injected into a sample loop connected to HiPrep Q FF 16/10 (GE healthcare, 28936543). The supernatant was injected at 2 mL/min and eluted using buffer B (20mM Tris-HCl ...