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Citations for GE Life Sciences :
1601 - 1650 of 4701 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Sample fractions were concentrated and size-exclusion chromatography (SEC) carried out using HiLoad 16/600 Superdex 200 pg or 75 pg columns (GE Healthcare) for UBA1a and UBC2 or UEV1 respectively ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were eluted with 5 column volumes elution buffer then purified by gel-filtration using an SD75 16/60 Superdex gel filtration column equilibrated in gel-filtration buffer (GF) with an AKTA pure (GE Healthcare). For SciW ...
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bioRxiv - Immunology 2021Quote: ... the soluble portion was concentrated and purified by chromatography on a Superdex 200 16/60 HiLoad size-exclusion column (GE Healthcare) followed by a Resource Q anion-exchange chromatography column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... Properly folded trimers were eluted with 3M MgCl2 and immediately dialyzed into storage buffer before being subjected to multiple size exclusion chromatography runs with a Superdex200 16/60 column followed by a Superose6 10/300 column (GE Healthcare). Peak fractions verified to be BG505 SOSIP.664 trimers were stored as individual fractions at 4°C in storage buffer.
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... The peak fraction (eluting at ~150 mM imidazole) was pooled and further purified over size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 8.0 and 150 mM NaCl) ...
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bioRxiv - Immunology 2021Quote: ... the remaining soluble portion of the complex was concentrated and then purified by chromatography on a Superdex 200 16/60 column followed by Resource Q anion-exchange chromatography (GE Healthcare), as previously described (36) ...
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bioRxiv - Genetics 2020Quote: ... The protein sample was finally purified on size-exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), pre-equilibrated with buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Size exclusion chromatography was carried out using a Biorad FPLC system with a HiLoad 16/60 Superdex 75 pg column (GE healthcare) pre-equilibrated with protein buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 kDa molecular weight cutoff) and purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), which was pre-equilibrated with buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... and the Csx30–Csx31–RpoE complex was purified by Ni-NTA Superflow resin and a HiLoad 16/600 Superdex 200 column (GE Healthcare). The target RNA (25 nucleotides plus 5′ GG ...
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bioRxiv - Microbiology 2022Quote: ... Both the 6His- and Strep-tagged proteins were then further purified by SEC on an ÄKTA Purifier using a Superdex 200 16/600 GL column (GE Healthcare), and simultaneously buffer-exchanged into 50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... followed by concentration in a Vivaspin 20 concentrator [10-kDa MWCO (molecular weight cutoff)] and final gel filtration step on a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was loaded on a Superdex 200 16/600 (GE-healthcare) size exclusion column connected to an AKTA Basic FPLC (GE Healthcare) in SEC-buffer ...
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bioRxiv - Biophysics 2022Quote: ... The His8-Sec14-enriched fractions were pooled and further resolved into two peaks by size exclusion chromatography (Superdex 75 16/600 column, GE Healthcare) at a flow rate of 1 mL/min in lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
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bioRxiv - Plant Biology 2022Quote: ... and injected onto a pre-equilibrated Hiload 16/600 Superdex 200 pg (in GF buffer) connected to an AKTApure system (GE Healthcare) set at 0.8 ml/min ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified MglA-His6 or His6-MglB proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer A without imidazole for use in GTPase assays or buffer C (20mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated VSGs were subjected to size exclusion chromatography as the last clean-up step using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Trimer was purified on a Superdex 200 Increase 10/300 GL column or a HiLoad 16/600 Superdex 200 PG column (GE Healthcare), whereas SApNPs were characterized on a Superose 6 10/300 GL column ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cleaved protein was separated from TEV protease by a subtractive Ni-NTA pass and loaded on to a HiLoad 16/60 Superdex 75 column (GE Healthcare) equilibrated with 20 mM Hepes ...
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bioRxiv - Immunology 2022Quote: ... and mi3 particles were isolated by ammonium sulfate precipitation followed by SEC purification using a HiLoad 16/600 Superdex 200 (GE Healthcare) column equilibrated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... We eluted bound proteins using an imidazole gradient and subjected them to size-exclusion chromatography (Superdex 200 16/600, GE Healthcare) using 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... centrifuge filters and subsequently applied to size exclusion chromatography (SEC) using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg, GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted fractions containing Tau441 were pooled and further purified by size exclusion chromatography (HiLoad 16/600 Superdex 200pg, GE Healthcare) in buffer (50 mM NaPO4 ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 25 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein were concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The protein was further purified (separating the NTD from the CTD) by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in 20 mM HEPES/NaOH pH=7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The protein sample was then purified using size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), previously equilibrated with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was concentrated and applied to a HiLoad 16/600 Superdex 200 pg size exclusion chromatography (SEC) column (GE Healthcare) equilibrated in p97 SEC buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... IgGs were expressed by transient transfection in Expi293 cells or HEK293-6E cells and purified from cell supernatants using MabSelect SURE (Cytiva) columns followed by SEC purification using a 10/300 or 16/600 Superdex 200 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... Tev protease and other contaminants were removed with Nickel NTA resin and the unbound protein purified further using a HiLoad 16/60 superdex 75 size exclusion column (GE Healthcare). His-GST-DIDATRX ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Biophysics 2023Quote: ... Further purification was performed by anion exchange chromatography using HiTrap Q HP column (Cytiva) and size-exclusion chromatography using Superdex 200 pg 16/600 column (GE Healthcare). Human GAC preparations were prepared as previously described25 ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences) pre-equilibrated with buffer F (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The tag-less proteins were separated from the tags and His6-TEV protease by Ni2+ affinity chromatography and were further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated with HSEC buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1mM DTT) and using a gradient of 30–1000 mM NaCl followed by size-exclusion chromatography (Superdex 75 16/60, GE Healthcare) in buffer B [25 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... the remaining sample was centrifuged at 16,000 x g for 10 min to remove any precipitate and loaded onto a HiPrep 16/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) pre-equilibrated in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... non-farnesylated constructs of GBP1 were purified by affinity chromatography (Ni Sepharose HP, Cytiva) and size exclusion chromatography (Superdex 200 16/600, GE Healthcare). Farnesylated GBP1 constructs were co-expressed with recombinant farnesyltransferase (FTase ...
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bioRxiv - Biophysics 2023Quote: VD1 hydrodynamic properties were approximated by SEC using a HiLoad 16/60 Superdex 75 prep grade (S-75) column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated and was further purified by a Superdex increase 200 16/600 size exclusion chromatography column (GE Healthcare) that was pre-equilibrated with SEC buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Immunology 2023Quote: ... followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Biochemistry 2022Quote: ... His-tagged HRV 3C protease (produced in-house) was used instead of TEV protease and a HiLoad 16/600 S200pg SEC column (GE Healthcare) was used instead of a HiLoad 26/600 S200 SEC column ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...