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Citations for GE Life Sciences :
1551 - 1600 of 4701 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Phl p 5 mannan neoglycoconjugates (P5-MN) were subjected to size exclusion chromatography using a 16/60 Sephacryl S-300 HR column (GE Healthcare) (Suppl ...
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bioRxiv - Biochemistry 2020Quote: ... The fusion protein was digested with thrombin (10 units/mg of TrxA-UbiTPa) at room temperature and then loaded on a HiLoad 16/600 Superdex 200 pg (GE Healthcare) coupled with a HisTrap FF crude column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The final size-exclusion chromatography after affinity purification and tag removal was performed using Superdex75 16/60 HiLoad and Superdex75 increase 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... α-Syn was purified by size exclusion chromatography using a HiLoad Superdex column 16/600 75 pg (GE healthcare life sciences) connected to an ÄKTA system (GE healthcare life sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... The refolded tetramer was centrifuged for 5 min at 13000 rcf at 4°C to eliminate any insoluble particles and purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 mL/min in 500mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The 176 bp GUB DNA was recovered from the supernatant of the polyethylene glycol precipitation fraction and further purified with gel-filtration chromatography on a HiLoad 16/60 Superdex 75 pg column (GE Healthcare) using TE buffer (10 mM Tris-HCl [pH 7.5] and 0.1 mM EDTA) ...
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bioRxiv - Cell Biology 2020Quote: ... and H3–H4 tetramer and H2A–H2B dimer were isolated with gel-filtration chromatography on a HiLoad 16/60 Superdex 75 pg column (GE Healthcare). Fractions containing (H3–H4 tetramer and H2A– H2B dimer ...
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bioRxiv - Systems Biology 2020Quote: ... Whole blood was diluted with room temperature RPMI medium in a 1:2 ratio to facilitate cell separation for other analyses using the SepMate PBMC isolation tubes (STEMCELL) containing 16 mL Ficoll (GE Healthcare). Diluted whole blood was centrifuged at 1200 g for 20 minutes at 20 °C ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... concentrated using centrifugal filters to bring down the volume to ∼1 mL and injected onto a Superdex 200 16/60 size exclusion column (GE Healthcare) previously equilibrated in HEPES 50 mM ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated protein was spun down 15 min in a bench-top centrifuge (4°C) and immediately loaded onto a HiLoad 16/600 Superdex 200 column (GE Healthcare), preequilibrated in gel filtration buffer consisting of 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... The excess dye was removed by loading the capsid sample on a HiPrep Sephacryl 16/60 size exclusion column (GE Healthcare), using the appropriate buffer (for MS2 and MS2S37P ...
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bioRxiv - Synthetic Biology 2020Quote: ... The gel filtration was performed with a HiPrep 16/60 Sephacryl S-100 column on an AKTA chromatography system (GE Healthcare), and the size of the proteins in each eluted fraction were verified by SDS-PAGE.
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bioRxiv - Plant Biology 2020Quote: ... and protease by IMAC and purified further by size-exclusion chromatography using either a HiLoad 16/600 Superdex 75 PG column or HiLoad 26/600 Superdex 75 PG column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 and 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted proteins were digested with 3C proteases at room temperature and further purified by size exclusion chromatography on HiLoadTM Superdex 75 16/60 prepgrade columns (GE Healthcare) equilibrated with 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were topped up to 1 ml with Buffer A and loaded onto a HiPrep 16/60 Sephacryl S-500 HR column (GE Healthcare). Buffer A was run at 1 ml/min and the flow-through was collected in 5 ml fractions ...
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bioRxiv - Biophysics 2020Quote: ... The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare) in NMR buffer (20 mM sodium phosphate pH 6.5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... the remaining soluble portion of the complex was concentrated and then purified by chromatography on a Superdex200 16/60 column followed by Resource-Q anion-exchange chromatography (GE Healthcare), as described previously (18).
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through fraction was further purified by HiTrap Q FF column and a Superdex 75 16/60 column (GE Healthcare). The pooled fractions were concentrated to 15 mg/ml in crystallization buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... MlaFB containing fractions eluted from the NiNTA column were pooled and concentrated before separation on a Superdex 200 16/60 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... applying a gradient of imidazole from 50 mM to 500 mM and further separated by gel filtration using an HiLoad 16/600 Superdex 200 (GE Healthcare) in equilibration buffer ...
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bioRxiv - Biochemistry 2019Quote: ... before the protein was eluted with wash buffer supplemented with 2.5 mM D-desthiobiotin and further purified by size-exclusion chromatography using a HiLoad (16/600) Superdex 200 pg column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble protein was injected on a HiLoad Sephadex 16/600 S200 (for constructs larger than ~25 kDa) or S75 (for constructs smaller than ~25 kDa) (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala, Sweden). All other reagents including buffers and solvents were of analytical grade.
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bioRxiv - Biochemistry 2021Quote: ... His-SUMO-Tau peptide was purified by affinity chromatography on Ni-NTA resin followed by size exclusion chromatography (Hiload 16/60, Superdex 75, prep grade, Cytiva) in SPR buffer (HBS-EP+, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The reaction was quenched with 10 mM DTT and free dye was separated by size exclusion chromatography on a HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions contained labelled protein were pooled and concentrated with Amicon Ultra-15 50 kDa cutoff centrifugal filter units (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... The purified recombinant proteins Spn2-BFP or BFP were concentrated to 2 ml and were further purified by SEC with a HiLoadTM 16/600 superdexTM 200 pg column (28-9893-35; GE Healthcare) in SEC buffer (50 mM Tris (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... The RBD containing flow through was further purified by SEC using a HiLoad 16/600 Superdex 200 pg column (GE healthcare). Proteins were flash frozen and stored at −80°C until use.
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bioRxiv - Cancer Biology 2021Quote: ... Unphosphorylated and phosphorylated samples were then concentrated and loaded separately onto a HiLoad 16/600 Superdex 200 SEC column (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The eluate containing the RBD was further purified by SEC using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) column pre-equilibrated in 2mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... was used to elute the protein using a binding buffer with 400 mM imidazole followed by size exclusion chromatography on a Superdex75 16/600 (GE Healthcare) column equilibrated in binding buffer ...
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bioRxiv - Immunology 2020Quote: ... was further purified by cation exchange and heparin affinity chromatography prior polishing by gel filtration through a Superdex 200 16/40 column (GE Healthcare), which was operated in 300 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... IF3 was purified similarly to IF1 using a cation exchange chromatography on a HiPrep CM FF 16/10 column (28-9365-42, GE Healthcare), however using a stronger gradient in Buffer A (0.1 to 1 M NH4Cl) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were first purified by Ni-NTA agarose beads and further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare) in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... spun at max speed for 30 min in a bench-top centrifuge (at 4°C) and finally loaded on a Superdex200 16/60 (GE Healthcare) previously equilibrated in MPS1 buffer (250 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... samples were concentrated in 10K MWCO centricons (PAL Corporation) (5000rpm [3214xg] at 4°C) and applied on a Superdex200 HiLoad 16/600 column (GE Healthcare) using 25 mM Hepes pH 7.6 ...
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bioRxiv - Biochemistry 2021Quote: For crystallization trials the ATAD2 BRD was further purified by gel-filtration using a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare). The purified ATAD2 BRD 966-1112 ...
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bioRxiv - Cell Biology 2021Quote: ... Undigested protein and TEV protease were removed by a second Ni-NTA step and flow through containing Pex5p were concentrated for gel filtration (Hiload 16/60 Superdex 200 pg, GE healthcare). Relevant fractions were pooled together and the protein was concentrated ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through fraction was concentrated and further purified by size exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in 20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was further purified using size-exclusion chromatography over a Superdex S75 13/300 or 16/600 column (GE Healthcare). For Ube2k-UBC containing the Avi tag ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated Arr samples were injected into a Superdex 75 16/60 size-exclusion chromatography (SEC) column (GE Healthcare, Chicago, IL, USA) pre-equilibrated in SEC buffer for removal of aggregated protein ...
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bioRxiv - Biophysics 2020Quote: ... The resulting samples were concentrated by using 100 kDa centrifugal filter devices and subjected to size-exclusion chromatography (Superdex 200 16/60; GE Healthcare) with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was further purified by a HiLoad® 16/600 Superdex® 200 pg gel filtration column (GE Healthcare) in buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... Fractions showing the corresponding encapsulin band on SDS-PAGE were pooled and subjected to further purification via anion-exchange chromatography using a HiPrep Q 16/10 column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... AtGDH sample was concentrated and further purified with the Superdex 200 pg 16/600 gel filtration column (120 ml bed volume, GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by size exclusion chromatography (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A as eluent ...
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bioRxiv - Immunology 2020Quote: ... Proteins were eluted from columns with 6M guanidine and subsequently loaded onto a gel filtration column (HiLoad 16/60 Superdex 200pg, GE Healthcare) to separate anti-OVA mAb heavy and light chains (FLC) ...
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bioRxiv - Microbiology 2020Quote: The Vag8:C1-INH complex was prepared in vitro by incubating C1-INH in ∼1.5 molar excess with Vag8 at room temperature for 10 min followed by purification using size exclusion chromatography on a S200pg 16/600 column (GE Healthcare). The eluted fractions were analysed by SDS-PAGE followed by ultrafiltration to concentrate the protein complex.
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bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
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bioRxiv - Microbiology 2020Quote: ... RBD with AviTag was biotinylated as described previously [32] and purified by size exclusion chromatography on a Hiload 16/90 Superdex 200 column (GE Healthcare) in 20 mM Tris-HCl pH 7.4 and 150 mM NaCl.