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Citations for GE Life Sciences :
1501 - 1550 of 4701 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... A second purification step was performed by loading Protein A eluate on a Hiload 16/600 Superdex 200 pg gel filtration column (GE Healthcare), which was equilibrated and run using DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted protein was then supplemented with 1mM TCEP and concentrated to be further purified by gel filtration on a HiLoad Superdex200 16/600 using an ÄKTA Pure (GE Healthcare). The gel filtration column was pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... The His6-tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: ... The affinity-purified protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the protein of interest were pooled together and loaded onto a pre-equilibrated size exclusion column (HiPrep 16/60 Sephacryl S-300, GE Healthcare). The protein was then eluted using HSU buffer and the protein fractions were used immediately or frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biophysics 2020Quote: ... the pH of the aSyn sample was adjusted to pH 7 and filtered through a 0.22 μm filter before being loaded onto a HiPrep Phenyl FF 16/10 (High Sub) column (GE Healthcare, Sweden) and eluted in HIC buffer A (50 mM Bis-Tris ...
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bioRxiv - Biophysics 2020Quote: ... Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare) in 20 mM sodium phosphate buffer (pH 7.3) ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing the Mtw1c were concentrated using a Vivaspin 20 ultracentrifugal unit MWCO 10000 Dalton (Satorius) and loaded to a HiLoad Superdex 200 16/600 pg (GE Healthcare) equilibrated in 30 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... The 6x His tag was removed by TEV protease digestion followed by passage over NiNTA followed by a final gel filtration chromatography on a Superdex 200 16/60 column (GE Healthcare). The final purified protein was concentrated to approximately 10 μM and snap frozen on liquid nitrogen in small aliquots.
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bioRxiv - Biochemistry 2022Quote: ... The flow-through fraction was concentrated and further purified by size exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in 20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2022Quote: ... the protein samples were then further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (ÄKTA pure, GE Healthcare). The desired fractions containing dimeric VP40 or monomeric LactC2 were collected ...
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bioRxiv - Cell Biology 2022Quote: ... then the protein was recovered in the flow-through and separated from thrombin and last contaminants using gel filtration (Superdex 200 pg HiLoad 16/600 column, GE healthcare). The final yield was typically 20 mg (LB ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was purified with subtractive Ni-NTA followed by purification on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The nickel affinity chromatography step was repeated once more with dialyzed CICmin prior to purification on a HiLoad Superdex 75 16/600 column (GE Healthcare) equilibrated with 50 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... coli was essentially performed as described 15 with the modification that 25 mM HEPES buffer was used as buffer component in all purification steps and the final gel filtration was performed on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 10 ml Strep-Tactin column (Iba) and a gel filtration column (Superdex200 16 600, GE Healthcare). The purified protein was stored in PBS containing 1 mM TCEP at -80°C ...
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bioRxiv - Cell Biology 2022Quote: ... Myristoylated Arf1 was enriched by ammonium sulfate precipitation (35% saturation) then applied to HiLoad 16/60 Superdex 75 column (GE Healthcare) in 20 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... K11-Ub4 was made from K11-Ub2 and purified by size exclusion chromatography using a Superdex 75 HiLoad 16/600 column (GE Healthcare). The yield for K11-Ub4 was about 10% ...
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bioRxiv - Immunology 2022Quote: ... The complex was purified from excess/unassembled material using size-exclusion chromatography (HiLoad® 16/600 Superdex® pg200 (GE Healthcare) column running TBS buffer (Alfa Aesar)) ...
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bioRxiv - Biophysics 2022Quote: ... K48 and K63-Ub4 required an additional purification step via size exclusion chromatography using a Superdex 75 HiLoad 16/600 column (GE Healthcare). K11-Ub2 was made with K63R as described by (Bremm et al. ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing PomXN_K13AR15A-His6 were pooled and concentrated with an Amicon Ultra-15 centrifugation filter device with a cutoff of 3kDa and loaded onto a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare) that was equilibrated with dialysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... concentrated and applied onto a size exclusion chromatography (SEC) column (SD200 increase 10/300 or SD200 16/600 pg, GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained structural proton images and DTI for assessment of volumes and diffusivity using a flexible 16-channel coil (GE Healthcare). Anatomy and volumes were evaluated using T1-weighted (TR/TE = 14/500 ms ...
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bioRxiv - Microbiology 2020Quote: ... a pool containing Staphopain A was directly loaded on a HiPrep CM Fast Flow 16/10 (#28-9365-42, GE Healthcare) column equilibrated with NaAc5.5 and the column was washed until OD280nm reached a baseline ...
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bioRxiv - Microbiology 2020Quote: ... and 5 mM EDTA were pooled and concentrated by ultrafiltration on Amicon 10 kDa cut-of membrane and loaded on HiLoad 16/60 Superdex 75 pg (#28-9893-33, GE Healthcare) equilibrated with NaAc5.5 and fractions containing the proteolytic activity on azocasein were pooled and concentrated as described above ...
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bioRxiv - Molecular Biology 2019Quote: ... RNAP was then mixed with an excess of σ70 and loaded onto a 16/60 Superdex 200 prep grade gel filtration column (GE Healthcare) with buffer A (20 mM TRIS pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The final purification of CwlQ-6His protein was conducted via size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using gel filtration buffer (25 mM Tris [pH 8.0] ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrated BASS-XL tetramer was applied on a HiLoad 16/600 Superdex 200 gel filtration column using an AKTA Purifier (GE Healthcare). Crystallization conditions were screened using a Mosquito Robot (TTP Labtech ...
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bioRxiv - Microbiology 2020Quote: ... concentrated using a Vivaspin 20 centrifugal concentrator (10-kDa molecular weight cutoff) and applied to a HiLoad 16/600 Superdex 75 pg gel filtration column (GE Healthcare). Pure protein samples were dialyzed against 10 mM TrisHCl pH 7.0 and concentrated as described above ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated protein was then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare, HiLoad 16/60) equilibrated with buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... SepH or FtsZMs was then concentrated and subjected to size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in Tris-FtsZ buffer ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified by gel filtration chromatography on a HiLoad™ 16/600 Superdex 200 pg column (GE Healthcare) with the following buffer ...
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bioRxiv - Immunology 2020Quote: ... Conditioned medium was clarified by centrifugation and protein was sequentially purified by a HisTrap HP column and a HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography (SEC) column (both from GE Healthcare). Purified protein was first analyzed by Native-PAGE and Western blot ...
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bioRxiv - Microbiology 2021Quote: ... HisTRAP eluted peak fractions were pooled and applied to a Hi-Load 16/600 Superdex-200 (preparation grade) size-exclusion column (GE Healthcare) equilibrated with the gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Capsid protein was eluted at 1 M imidazole in NTE buffer and the collected elute was subjected to size exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare). The fraction containing DENV2C protein were pooled and dialyzed against 50 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The peak fractions were collected and purified by size exclusion chromatography using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Peak protein fractions were pooled and loaded onto the size-exclusion chromatography column using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing His6-LexA were pooled and subjected to size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in 50mM Tris pH 10.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... size-exclusion chromatography of all proteins was carried out on either HiLoad 26/60 or 16/600 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... GST- DIDATRX fragments and His-DIDATRX proteins were purified directly after Ni-NTA using HiLoad 16/60 superdex 200 or 75 size exclusion columns (GE Healthcare). With the exception of proteins used in NMR experiments ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by incubating the purified proteins with ∼3% (w/w) thrombin (4°C, overnight) and then subjected to SEC using a Superdex S-200 16/60 column (GE Healthcare) pre-equilibrated with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The pooled fractions containing tdClamp-HaloTag were purified by size exclusion chromatography on a HighLoad Superdex 200 pg 16/60 column (GE Healthcare). Aliquots from combined fractions were frozen in liquid nitrogen and stored at -80°C until usage.
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bioRxiv - Biochemistry 2019Quote: ... The proteins were then subjected to size-exclusion chromatography using a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare) equilibrated in SEC buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... Orn was concentrated by Amicon Ultra 10K concentrator prior to loading onto a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2019Quote: ... aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare). aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA ...
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bioRxiv - Biophysics 2020Quote: ... and the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), equilibrated with buffer E (20 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... and the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), equilibrated with buffer B ...
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bioRxiv - Biochemistry 2019Quote: ... the beads were centrifuged and the supernatant was run over a HiPrep Sephacryl 16/60 S-100 HR column (GE Healthcare) in Crystal Buffer 1 (10 mM Tris-HCl pH 8.3 ...