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Citations for GE Life Sciences :
6951 - 7000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Protein was affinity purified from lysate using a HisTrap HP 5 mL column (GE Healthcare) in binding buffer (50 mM NaPO4 ...
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bioRxiv - Biophysics 2022Quote: ... 89% (v/v) Percoll (GE healthcare), 1 cOmplete™ EDTA-free Protease Inhibitor ...
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bioRxiv - Biophysics 2022Quote: All samples were pre-reduced with 5 mM DTT for one hour followed by desalting using a PD10 column (GE Healthcare) into 20 mM tris pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... ion exchange chromatography for initial protein purification was performed on a 1ml HiTrap SP FF (GE healthcare) and a 24ml Superdex 75 10 300 GL (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... After purification with Ni-NTA agarose the protein was diluted to 375mM NaCl and further purified on an ÄKTA purifier system (GE Healthcare Life Sciences) at 4 °C using 1ml HiTrap™ Heparin HP column (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... and a 24ml Superdex 75 10 300 GL (GE healthcare) was used for size-exclusion chromatography ...
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bioRxiv - Biophysics 2022Quote: ... Unencapsulated protein was removed by desalting on Sepharose CL-4B (GE Healthcare). Liposomes were attached via streptavidin to a quartz slide passivated with biotinylated-BSA ...
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bioRxiv - Biophysics 2022Quote: ... Unconjugated dye was removed by desalting with Sephadex G50 (GE Healthcare) followed by dialysis.
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bioRxiv - Biophysics 2022Quote: ... followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL, GE Healthcare). Purified protein was diluted in PBS buffer (pH = 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Images were taken using a Quant LAS 4000 platform (GE Healthcare Life Sciences, USA)
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bioRxiv - Biophysics 2022Quote: ... Samples were imaged using the DeltaVision Elite deconvolution microscope (Applied Precision, Issaquah, WA, USA). Images were deconvolved using the aggressive algorithm in the DeltaVision softWoRx program.
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bioRxiv - Biophysics 2022Quote: ... Proteins were further purified by ion-exchange column (Source-15Q, GE healthcare) and polished by gel-filtration column (Superdex-200 ...
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bioRxiv - Biophysics 2022Quote: ... pooled fractions from the first gel-filtration purification were further purified by anion exchange column (Source-15Q, GE Healthcare) and then subjected to a second gel-filtration column in SEC150 buffer ...
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bioRxiv - Biophysics 2022Quote: ... and 5mg/mL (NNDP52, CNDP52 and ZF2) were loaded onto a Superdex 200 (30 x 1cm) analytical gel filtration column (GE Healthcare), equilibrated in 50mM Tris pH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Eluted Sox2 was refolded by changing to a zero-urea buffer using a desalting column (GE healthcare #17-1408-01). Further purification was performed by gel filtration on a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Further purification was performed by gel filtration on a Superdex 200 10/300 GL column (GE Healthcare). Fluorescence labeling was performed as previously described.32 In brief ...
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bioRxiv - Biophysics 2022Quote: ... Cy5 or Cy3 maleimide (GE healthcare) was mixed with Sox2 at a molar ratio of ~2:1 ...
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bioRxiv - Biophysics 2022Quote: ... Cell disruption was achieved by sonication (Branson Sonifier SFX250) and the soluble fraction was subjected to immobilized metal affinity purification using a 5 ml Ni-NTA column (GE healthcare) connected to a FPLC system (GE healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... reactions were diluted with water (970 μl) and purified by desalting on a disposable gel-filtration column (NAP10, GE Healthcare Life Sciences, cat. no. GE17-0854-02) according to the manufacturer’s instruction to remove the majority of the free dye ...
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bioRxiv - Biophysics 2022Quote: ... The images were processed using ImageQuant TL v8.1.0.0 (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: Tubulin from bovine brain (PurSolutions) was labeled with Cy5 NHS ester (GE Healthcare) as previously described51.
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bioRxiv - Biophysics 2022Quote: ... connected to a FPLC system (GE healthcare). The 6xHis tag was cleaved from the N-terminus of p51 using a 1:10 mass ratio of HRV14 3C protease by overnight treatment on ice ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The individual complexes were incubated over ice for 1 hour and purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE healthcare) pre-equilibrated in a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Western blots were developed by chemiluminescence (Super Signal West Dura, Pierce Thermo) and imaged using an Amersham Imager 600 imager (GE Healthcare Life Sciences). Bands were quantified with Gel-Pro Analyzer 4 (Meyer Instruments).
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were anti-rabbit (NA934V, GE Healthcare) or anti-mouse (NA931V ...
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bioRxiv - Cell Biology 2022Quote: ... These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime, GE healthcare). Chemiluminescence signals were detected by a LAS 4000 (GE healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Chemiluminescence signals were detected by a LAS 4000 (GE healthcare) and protein levels were quantified using ImageQuant software (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... and protein levels were quantified using ImageQuant software (GE Healthcare). Results were normalized to GAPDH and then to Sham values.
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bioRxiv - Cell Biology 2022Quote: ... or anti-mouse (NA931V, GE Healthcare). These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime ...
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bioRxiv - Biophysics 2022Quote: ... The NaCl concentration of the eluate was adjusted to 75 mM with IE-A buffer and applied to a 5mL HiTrap Q Sepharose anion exchange column (GE Healthcare) and eluted with a linear gradient of 75 mM to 1 M NaCl (IE-B buffer) ...
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bioRxiv - Biophysics 2022Quote: SPR analysis were carried out at 25.0 °C using Biacore T100 system (GE Healthcare, Chicago, IL, USA). In all protocols ...
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bioRxiv - Biophysics 2022Quote: ... and polished by gel-filtration column (Superdex-200, GE Healthcare) in SEC150 buffer without protease inhibitors ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and applied to an SEC step using HiLoad 16/60 Superdex 75 (GE Healthcare, Chicago IL) in SEC75 buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The sensor chips for the SPR measurements were purchased from GE Healthcare (Little Chalfont, Buckinghamshire, UK). Any other reagents and materials were all purchased from FUJIFILM Wako Pure Chemical Corp ...
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bioRxiv - Biophysics 2022Quote: ... The cleared lysate was applied into glutathione-Sepharose 4B resin (GE Healthcare, Piscataway, NJ) overnight in 4°C on a nutator ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and then applied to an initial size-exclusion chromatography (SEC) step using HiLoad 16/60 Superdex 200 (GE Healthcare, Chicago IL) in SEC200 buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... ImageQuant LAS4000 (GE Healthcare Europe, Freiburg, Germany). Primary antibodies used are as follows ...
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bioRxiv - Biophysics 2022Quote: ... 25 μL of this mixture was then chromatographed at room temperature and a flow rate of 0.04 mL/min on a Superdex-200 3.2/300 size exclusion column equilibrated in assembly buffer (GE Healthcare Ettan). A 50 μL fraction containing the largest molecular weight complex was assessed by SDS-PAGE (stained with SYPRO Red [Thermo Fisher] ...
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bioRxiv - Biophysics 2022Quote: ... using HisTrap FF columns (GE Healthcare). Protein fractions were further purified using Size Exclusion Chromatography ...
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bioRxiv - Biophysics 2022Quote: ... and purified under denaturing conditions using Q and SP ion exchange columns (GE Healthcare). Octamers were refolded by dialysis and purified by gel filtration on a Superdex 200 10/300 GL column ...
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bioRxiv - Biophysics 2022Quote: ... using a Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... and visualized using a Typhoon FLA7000 gel imager (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Applied method for purification in Newcastle was routine in lab as previously described 50% and 100% discontinuous Percoll (GE Healthcare, Castle Hill, Australia) centrifugation gradients for normal sperm11 ...
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bioRxiv - Cell Biology 2022Quote: ... A cellulose acetate membrane (0.2 µm pore size, GE Healthcare) was pre-equilibrated in 0.1% SDS and affixed to the filter trap apparatus (PR648 Slot Blot Blotting Manifold ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and ribosome protected fragments were isolated using sephacryl S400 columns (GE Healthcare) in TE buffer ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...