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Citations for GE Life Sciences :
7051 - 7100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by centrifugation using Ficoll-Paque PLUS (GE Healthcare). PBMCs were resuspended in R10 media consisting of RPMI-1640 (Wisent) ...
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bioRxiv - Immunology 2022Quote: ... Each target was immobilized onto flow cells in a CM5 sensor chip (GE Healthcare) via the amine-coupling method ...
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bioRxiv - Immunology 2022Quote: ... The binding curves were analysed using a kinetic binding model supplied with Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... followed by Superdex 75 (GE Healthcare). Tags were removed by Enterokinase (Roche ...
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bioRxiv - Immunology 2022Quote: ... and purified by Ni Sepharose Excel (GE Healthcare) followed by Superdex 75 (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected from selected hybridoma clones and purified through a protein G-sepharose column (GE Healthcare). mAbs were isotyped with Pierce rapid isotyping kit (Cat#26178) ...
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bioRxiv - Immunology 2022Quote: ... SPR based assays were performed using the Biacore™ T200 (GE Healthcare) system and the kinetic rate constants (ka and kd ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Immunology 2022Quote: ... blood was diluted in PBS and fractions were separated by Ficoll-Paque (GE healthcare, Zeist, The Netherlands) density gradient centrifugation as previously described (Domínguez-Andrés et al. ...
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bioRxiv - Immunology 2022Quote: ... The cell free supernatant derived during harvest of the fermented broth was processed using Blue Sepharose™ 6 Fast Flow column (GE Healthcare, Cat# 17-0948-01) in flow-through mode followed by a hydrophobic interaction chromatography (HIC ...
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bioRxiv - Microbiology 2022Quote: ... rolling at 4°C with 100 μl of Ni-NTA Agarose (GE Healthcare) pre-equilibrated with the SEC buffer ...
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bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: ... and loaded onto the Superdex 200 Increase 10/300 GL (GE Healthcare) equilibrated with the final SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... the sample was concentrated to 5 ml and loaded over the HiLoad 26/600 Superdex 200 prep grade (GE Healthcare). In order to clear the impurities which had been eluted with the complex ...
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bioRxiv - Microbiology 2022Quote: ... Cells were separated from supernatant by centrifugation and supernatant containing the secreted proteins was loaded onto a NiFF (GE Healthcare) column as described above.
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bioRxiv - Microbiology 2022Quote: ... The concentrated medium was purified using a HiTrap protein A column (GE Healthcare) as previously described (41 ...
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bioRxiv - Microbiology 2022Quote: ... The plates were scored after 24 and 48 h incubation at 37 °C and imaged using ImageQuant LAS 4000 (GE Healthcare). The last time point (24 h ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM 2-mercaptoethanol and refolded on a NiFF (GE Healthcare) column using a reverse linear urea gradient with imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... The sample was then applied to a AEKTA start chromatography system (GE Healthcare Europe GmbH, Freiburg, Germany), and purification of crosslinked proteins was achieved on a 1 ml HisTrap™ Fast Flow column ...
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bioRxiv - Biophysics 2022Quote: ... following a 30 minutes incubation with horseradish peroxidase-conjugated anti-M13 monoclonal antibody (GE Healthcare). Absorbance was measured at 450 nm after quenching the reaction with 1.0 M HCl ...
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bioRxiv - Biophysics 2022Quote: ... Eluted COPs were loaded onto a 1 mL Resource S column (GE Healthcare) equilibrated with COP Buffer A (50 mM sodium acetate pH 5.0 ...
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bioRxiv - Biophysics 2022Quote: ... Supernatants were filtered by 0.45 μm and loaded onto a 5 mL HiTrap MabSelect SuRe column (GE Healthcare) equilibrated with COP Wash buffer (20 mM Hepes pH 7.4 and 500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
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bioRxiv - Biophysics 2022Quote: ... and size-exclusion chromatography was performed using an AKTA pure system (GE Healthcare) in order to separate free Cal-520 dye molecules from loaded vesicles.
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bioRxiv - Bioengineering 2022Quote: ... purified protein (100 µL) was applied to Superdex 200 GL column (GE HealthCare) at 0.5 mL/min using a UFLC system (Shimadzu ...
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bioRxiv - Bioengineering 2022Quote: ... The assembled 6RBD-np was purified by size exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare) in DPBS ...
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bioRxiv - Bioengineering 2022Quote: ... connected to an Ä KTA FPLC system (GE HealthCare). The purified S-PCNA1 and M-PCNA2 were mixed with a 1.2:1 molar ratio at 4°C for 6 hrs ...
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bioRxiv - Bioengineering 2022Quote: Drosophila Kc167 cells were cultured in Hyclone CCM3 Insect Media (GE Life Sciences) containing 1% Pen-Strep (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: The shift in elution volume of the antigens was determined by size exclusion chromatography (SEC) on a Superdex 200 GL column (GE HealthCare) equilibrated with 50 mM Tris (pH 8.0 ...
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bioRxiv - Bioengineering 2022Quote: ... and by size exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare, Uppsala, Sweden) in Dulbecco′s phosphate buffered saline (DPBS) ...
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bioRxiv - Bioengineering 2022Quote: T1-weighted axial images of the non-dominant arm were obtained using a 3T MRI scanner (Discovery MR750w, GE Healthcare, Chicago, IL, USA) and a receiver 16-channel flex coil ...
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bioRxiv - Biochemistry 2022Quote: ... followed by further separation from small molecules by passage through a pre-packed PD-10 column (GE Healthcare). Protein concentrations were determined by absorbance at 280 nm using a calculated molar extinction coefficient (ε280nm ...
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bioRxiv - Bioengineering 2022Quote: ... Further purification was performed by ion-exchange chromatography using mono Q 5/50 GL (GE HealthCare, Uppsala, Sweden) for each fusion protein in 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... and isolated using the Ficoll Paque Plus density gradient medium (GE Healthcare Life Sciences) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was loaded into a 5 mL HisTrap FF column charged with Ni2+ (GE Healthcare). The protein was eluted using a buffer containing 10 mM tris (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... Bands from immunoprecipitated samples were visualized using an Amersham Imager 600 (GE Healthcare), and the protein band at 110 kDa for C55R F3 was manually excised ...
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bioRxiv - Cancer Biology 2022Quote: ... The plates were scanned with phosphor-imager (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Immunoreactive protein bands were visualized via enhanced chemiluminescence and scanned with a LAS-4000 system (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
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bioRxiv - Cell Biology 2022Quote: ... according to the manufacturer’s instructions and imaged on a Typhoon FLA 9500 biomolecular imager (GE Healthcare). TRARG1 bands were excised for in-gel digestion.
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bioRxiv - Biochemistry 2022Quote: ... Individual tRNA species were purified using biotinylated DNA probes anchored to a high-capacity streptavidin agarose resin (GE Healthcare). Typically for this purification ...
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bioRxiv - Cell Biology 2022Quote: ... In a 24 well plate, 65μL siRNA [50nM] for sequences, see (Jongsma et al., 2016)) was mixed with 26uL 1x siRNA buffer (GE Healthcare) containing 1.15uL Dharmafect 1 transfection reagent ...
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bioRxiv - Cell Biology 2022Quote: ... The mixture was analyzed by size exclusion chromatography using Superose 6 Increase 5/150 GL column (GE Healthcare) mounted on an AKTApure system ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by ion-exchange chromatography using the HiTrap Heparin HP column (GE Healthcare Life Sciences) and a Mono Q anion exchange column (16/10 ...
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bioRxiv - Biochemistry 2022Quote: ... and a Mono Q anion exchange column (16/10; GE Healthcare Life Sciences) using gradient elution from 150 mM to 2 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... 1991 and separated on a Mono STM5/50 GL Column (GE Healthcare, Uppsala, Sweden) fractions were eluted in 6 M urea ...
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bioRxiv - Biochemistry 2022Quote: ... The elution was collected and loaded onto the HiLoad 16/600 Superdex 200 (GE Healthcare) equilibrated in SEC buffer (10mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 was further purified by gel filtration on a 16/600 Superdex 200 column (GE Healthcare) in S200 buffer ...