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Citations for GE Life Sciences :
6801 - 6850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Further purification was carried out using size-exclusion chromatography with a Superose 6 10/300 column (GE Healthcare) in the buffer containing 20 mM HEPES pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... The obtained Fabs were purified with a Desalting column (GE Healthcare Life Sciences) to remove L-cysteine ...
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bioRxiv - Immunology 2022Quote: ... BG505 Env trimer was purified from transfected cell supernatants as described47 by 2G12 immunoaffinity and size-exclusion chromatography (SEC) with a Superose 6 16/600 column followed by Superdex 200 Increase 10/300 GL column (GE Life Sciences).
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Physiology 2022Quote: ... 75 V) and gel image was taken using an Image Quant LAS 500 chemo luminescence CCD camera (GE healthcare, life sciences, Chicago, IL, USA).
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bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... and further purified using size-exclusion chromatography using Superdex 200 (GE Healthcare).
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bioRxiv - Immunology 2022Quote: SPR experiments were performed using a Biacore® T100 instrument (GE Healthcare, Uppsala, Sweden). All binding analyses were performed at 25 °C using HBS-EP+ (10 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... at a dilution of 1:2000 was done for 1 hour at RT in blocking buffer and detection was done using ECL detection kit (RPN2235, GE Healthcare, USA). Images were taken by the ImageQuant LAS500 (GE healthcare ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2022Quote: ... Images were taken by the ImageQuant LAS500 (GE healthcare, life sciences, USA) and relative intensity of protein bands was quantified using ImageJ (Schneider ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, 15269894) for blotting at 80V for 60 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... 1mM DTT) and loaded onto a 5 ml Hitrap Q sepharose anion exchange column (GE Healthcare). A gradient from 0 % to 100 % high salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... 20% MetOH) to a nitrocellulose membrane (Amersham™ Protran™ 0,45μm NC, GE Healthcare Lifescience). Quality of transfer was checked by Ponceau-S (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was stained with Images were acquired on a DeltaVision Core deconvolution microscope (Applied Precision) equipped with a CoolSnap HQ2 charge-coupled device camera (Photometrics) ...
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bioRxiv - Cell Biology 2022Quote: ... and 3T3 cells were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (GE Healthcare), 100 U/ml penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science). Later ...
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bioRxiv - Biophysics 2022Quote: ... crude lysates were purified on a HiTtap TALON column (GE Healthcare; Chicago, IL), cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200 ...
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bioRxiv - Biophysics 2022Quote: ... cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200, GE Healthcare). The final product was collected in 25 mM HEPES pH 7.8 ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% fetal bovine serum (GE Healthcare), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2022Quote: Images for immunolabelled cells on micropatterns were acquired with an inverted wide field Deltavision Core Microscope (Applied Precision) equipped with highly sensitive cooled interlined charge-coupled device (CCD ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was collected and applied to calmodulin sepharose (GE Healthcare) pre-washed with binding buffer (10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were scanned using a Typhoon FLA9500 Imaging system and quantitated using ImageQuant TL software (GE Healthcare). Bands representing remodelled mononucleosomes were normalised to their respective input lanes.
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was incubated with pre-washed IgG sepharose (GE Healthcare) and incubated at 4 °C for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were scanned using a Typhoon Imaging FLA9500 system and quantitated using ImageQuant TL software (GE Healthcare). Mononucleosome bands were quantitated for each lane ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were visualised by scanning on a Typhoon FLA9500 Imaging system and quantitated using ImageQuant TL software (GE Healthcare). DNA bands were quantitated for each lane ...
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bioRxiv - Biophysics 2022Quote: ... All Cy5-labeled DNA was detected using Fujifilm FLA-9000 (GE Healthcare). Competition assays using specific and nonspecific oligos were performed as previously described [11] using 0.2-4 μM of competitor ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare, Pittsburgh, PA) [34] ...
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bioRxiv - Cell Biology 2022Quote: ... both supplemented with 10% fetal bovine serum (FBS, GE Healthcare) and 1% penicillin and streptomycin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The eluted proteins were further purified by Superdex 75 (GE Healthcare, USA) and stored in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... we isolated PBMCs from EDTA-anticoagulated blood by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare), removing contaminating red blood cells with ACK lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were added to Ni-NTA resin (GE Healthcare, USA). The nonspecific contaminants were eluted by washing the resin with the lysis buffer containing 10 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... Nb22 was eluted and purified by Superdex 75 (GE Healthcare, USA).
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bioRxiv - Immunology 2022Quote: ... Plasma was separated by centrifugation at 1100 rpm for 10 minutes and PBMC was isolated from the buffy coat by Ficoll-Paque PLUS (GE Healthcare) and cryopreserved in fetal calf serum + 10% DMSO.
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bioRxiv - Biophysics 2022Quote: ... The pooled and dialyzed protein solution from the metal chelate affinity chromatography was loaded on a 120 ml HiLoad® 16/600 Superdex® 75 pg column (GE Healthcare, Germany). The column was equilibrated in buffer (20 mM Tris ...
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bioRxiv - Biophysics 2022Quote: ... After removal of cell debris by centrifugation for 60 min at 50’000xg (25’000 rpm using 45 Ti rotor) the supernatant was loaded on 5ml HiTrap chelating column (GE Healthcare, Germany) running on AKTA prime liquid chromatography system ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.4 with a PD-10 desalting column (GE Healthcare Life). Protein concentration was determined by measuring A280 with a molar extinction coefficient of 20,400 M-1cm-1.
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bioRxiv - Biophysics 2022Quote: ... The His6-α2I domain was purified using a Ni2+-charged HisTrap HP (GE Healthcare Life) and buffer exchanged to PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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bioRxiv - Physiology 2022Quote: ... Whole-body and leg composition were calculated using software (enCORE Forma v. 15, GE Healthcare Lunar, Buckinghamshire, UK).
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bioRxiv - Neuroscience 2022Quote: 3D T1-weighted brain volumes were acquired at 1.5 Tesla (Signa, GE Healthcare) using a 3D Magnetization-Prepared Gradient-Echo BRAVO sequence (TR/TE/TI 8.2/3.1/450 ms ...
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bioRxiv - Physiology 2022Quote: ... body composition was measured by dual-energy X-ray absorptiometry (DXA) scans (Lunar iDXA, GE Healthcare, GE Medical systems, Belgium). Then ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... toxins were purified through Ni2+-affinity chromatography using a HiTrap Heparin HP column (GE Healthcare). Protein capture was verified via visualization of a single band on SDS-PAGE following Coomassie blue staining ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein was applied to a Resource Q column (GE Healthcare). The column was washed with a 130–200 mM NaCl gradient in Binding Buffer over 7 column volumes ...
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bioRxiv - Molecular Biology 2022Quote: ... Precipitated H-NS was dissolved in nickel binding buffer and applied to a HisTrap Ni2+ column (GE Healthcare). Fractions containing H-NS were mixed with TEV protease to remove the N-terminus His6x-tag ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS containing cell lysate was directly applied to a HiTrap Heparin HP column (GE Healthcare) equilibrated with Binding Buffer with 130 mM NaCl ...