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Citations for GE Life Sciences :
7201 - 7250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µl of Protein A agarose (GE Healthcare) was coupled with 2 µg of purified antibodies or 5 µl of antiserum ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were blot-transferred to a nylon membrane (Amersham Hybond-N+ #RPN203B) and probed with pBR322 DNA sequences labelled with AlkPhos Direct (GE Healthcare® #GERPN3680). Chemiluminescent signals of increasing exposition periods were recorded with a cooled CCD camera (KODAK Gel Logic 1500 Imaging System ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% (v/v) glycerol) before loading on to a third affinity column (HiTrap Heparin HP, GE Healthcare). A step gradient (25-50-75-100% ...
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bioRxiv - Molecular Biology 2022Quote: Immunostained cells were imaged on Delta Vision Core system (Applied Precision / GE Healthcare, Issaquah, WA) consisting of Olympus IX70 inverted microscope (Olympus America ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was desalted using a 5 ml HiTrap Desalting column (GE Healthcare) and sterile-filtered PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare). The infectivity titer of each virus (expressed as transduced units/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and TGF-β1 (5 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR product was inserted into pGEX- 5X-1 (GE Healthcare). GST fusion proteins were purified as described (39) ...
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bioRxiv - Bioengineering 2022Quote: ... Images were acquired using an Amersham Imager 600 (GE Healthcare), and contrast adjusted with a curves layer.
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bioRxiv - Neuroscience 2022Quote: ... and detected using ImageQuant LAS4000 (GE healthcare, USA). For reprobing ...
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bioRxiv - Cancer Biology 2022Quote: TS3132 was previously isolated from Pten/Smad4 mice on a mixed background 33 and cultured in DMEM (GE Healthcare, SH30243.FS) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... filter and size-exclusion chromatography at 4 °C on a Superdex 200 10/300 GL gel filtration column (GE Healthcare). Purity of the proteins was evaluated on SDS-PAGE gel ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... to approximately 3 – 4 ml and further purified by size-exclusion chromatography at 18 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare). His-TEV-CobB ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Molecular Biology 2022Quote: ... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies were detected using an ECL detection reagent and imaged on the ImageQuant LAS 4000 or Amersham Imager 600 (GE Healthcare Life Sciences). All quantitative analysis derived from western blotting was performed using densitometry analysis in ImageJ.
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bioRxiv - Microbiology 2022Quote: ... the protein samples were then further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (ÄKTA pure, GE Healthcare). The desired fractions containing dimeric VP40 or monomeric LactC2 were collected ...
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... was bound to 20 μL FastFlow Protein A Sepharose bead slurry (GE Healthcare) for 3 hours rotating at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... bone marrow mononuclear cells (BMMCs) were isolated by Ficoll-Paque Plus (GE healthcare) density gradient centrifugation and stained using CD34 (clone 8G12 ...
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bioRxiv - Biophysics 2022Quote: ... The Sumo tag was separated using an Ni-NTA column followed by HiLoad Superdex 75 PG gel filtration column (28-9893-34, GE Healthcare) if the protein was not pure as assessed by SDS-PAGE ...
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were imaged using enhanced chemiluminescence (ECL) reagent (GE Healthcare). For total protein staining of blots ...
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bioRxiv - Biochemistry 2022Quote: ... samples were run over a Superdex 200 26/60 size-exclusion column (GE Healthcare) equilibrated with HEPES buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The flow-through was concentrated to ∼3mg/mL (100kDa MWCO) and subjected to size exclusion chromatography on Superdex 200 increase 10/300 column (GE healthcare) in size exclusion buffer containing 50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Neuroscience 2022Quote: Magnetic resonance imaging was performed with a 3T GE Discovery MR750 MRI scanner (GE Healthcare, Milwaukee, WI) and a 32-channel head coil at the Cornell Magnetic Resonance Imaging Facility in Ithaca ...
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bioRxiv - Cell Biology 2022Quote: ... and added to 0.625 mL of glutathione-sepharose beads (GE Healthcare, GE17-0756), which were pre- equilibrated in equilibration buffer (1X PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Microbiology 2022Quote: ... His-PstPcWTMtb and all its phospho-mimetic variants are 34.1KDa and all of them eluted at the same column volume when run on the size exclusion resin (GE Healthcare Sephacryl S-200 in HiPrep 26/70 column). The purification chromatograms were analysed with a standard curve made from running protein standards (BIORAD ...
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bioRxiv - Microbiology 2022Quote: ... and run over size exclusion resin (GE Healthcare Sephacryl S-200 in HiPrep 26/70 column). His-FhaAMtb was purified using the buffers described in (Roumestand et al. ...
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bioRxiv - Immunology 2022Quote: ... and projected using the softWoRx software (Applied Precision, USA).
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bioRxiv - Microbiology 2022Quote: ... HeLa and HEK293T/17 cells lines were purchased from ATCC (Manassas, VA) and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal clone III (FC3; GE Healthcare Lifesciences, UT) and 1% Penicillin-Streptomycin (Pen Strep ...
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bioRxiv - Neuroscience 2022Quote: ... and transferred to a nitrocellulose membrane (GE Healthcare). Membrane was blocked (Blocking Buffer for Fluorescent Western Blotting ...
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bioRxiv - Biochemistry 2022Quote: ... The GYS1:GYG1 complexes were purified by affinity (Ni-Sepharose; GE Healthcare) and size-exclusion (Superose 6 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2022Quote: ... DNA products from a 48-well PCR plate (50 - 100 µL reaction per well) were pooled and injected into an anion exchange column (MonoQ-GL, GE Healthcare Life Sciences, Marlborough, MA, USA) for purification ...
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bioRxiv - Immunology 2022Quote: ... The secreted protein was purified from pre-conditioned media supernatant with Ni-NTA resin and purified via size exclusion chromatography with a S200 increase column on an AKTA PURE FPLC (GE Healthcare). To improve protein yields ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The protein sample was further purified using a Superdex200 26/600 size-exclusion chromatography column (GE Healthcare), and fractions were collected and concentrated to 1.8 mg/ml in 10 mM Tris pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... Bone marrow mononuclear cells (BMMC) were separated by Ficoll-Paque PLUS (GE Healthcare), and MM cells were enriched by CD138-positive selection with magnetic activated cell separation microbeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were developed by exposure to high-performance chemiluminescence films (Amersham Hyperfilm ECL, GE Healthcare) or in an ImageQuant LAS 4000 mini machine (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... defined (HyClone, GE Healthcare SH30070.03); 50 U/mL penicillin ...
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bioRxiv - Biophysics 2022Quote: SAXS data were collected with an AKTAmicro (GE Healthcare) FPLC coupled to a BioXolver L SAXS system (Xenocs ...
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bioRxiv - Immunology 2022Quote: ... plus ATP (100 μM) (GE healthcare). After mixing ...