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Citations for GE Life Sciences :
6851 - 6900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 5% glycerol) using PD SpinTrap G-25 columns (GE Healthcare #28-9180-04) pre-equilibrated with conjugation buffer ...
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bioRxiv - Cell Biology 2022Quote: ... before being transferred to nitrocellulose membranes (GE Healthcare, Amersham, UK). After probing with primary and secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... slower migrating band on a 20% polyacryamide PhastGel (GE Healthcare). Fe2+·EDTA–H-NS was stored in aliquots at −80°C and remained stable under these storage conditions for up to 1 year ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS C21S was then applied to a HiTrap Hep column (GE Healthcare) and eluted with Heparin Buffer containing 1 M NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1x penicillin/streptomycin (GE Healthcare, cat #SV30010).
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2022Quote: ... and a luminescent detector (Amersham Imager 600, GE Healthcare Life Science). Origins and recipes of all buffers and reagents are noted in the Key Resource Table.
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bioRxiv - Biochemistry 2022Quote: ... pH 4.0 by gel filtration chromatography using a PD-10 column (GE Healthcare), and concentrated to a final protein concentration of 1.0 – 1.5 mM in 250 μL using a Vivaspin microfiltration device and placed in a 5 mm Shigemi reduced volume NMR tube for measurements ...
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bioRxiv - Cancer Biology 2022Quote: ... or mouse (1:20000 dilution, NA9310; GE Healthcare Life Science) were incubated at room temperature with 1%–5% skimmed milk for 1 h ...
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bioRxiv - Physiology 2022Quote: ... The western blot was developed using Amersham ECL kit Start or Prime (Cat# RPN3243 or # RPN2232, GE life sciences).
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies for rabbit (1:20000 dilution, NA9340; GE healthcare Life science) or mouse (1:20000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant segment was purified by Ni2+-affinity chromatography using HisTrap HP purification columns with a FPLC system (ÄKTA Purifier, GE Healthcare) with elution buffer consisting of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Molecular Biology 2022Quote: ... for hours to days depending on the signal strength and imaged with a 635 nm laser scanner (Typhoon FLA9500, GE Life Sciences or Amersham Typhoon) with 750 V and 100 μm pixel size ...
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bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
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bioRxiv - Bioengineering 2022Quote: ... and loaded on a HiLoad® 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in 50 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 35 μL of each sample at the concentrations indicated in the Table were injected into a Superdex 200 PC 3.2/300 Increase column (GE Healthcare), pre-equilibrated with 25 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... The diluted supernatant was loaded onto a 5 mL Heparin HiTrap™ column (GE Healthcare) and eluted with a gradient up to 1 M NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... the first step purification was done on a 5 mL HisTrap™ HP column (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: Affinity constants were measured using a Biacore T200 (GE Healthcare) at 25 °C using PBST (PBS + 0.05% Tween 20 ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies were purchased from GE Healthcare (Waukesha, WI). DTT was purchased from Thermo Fisher Scientific (Ottawa ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 x 5 min in 0.05% PBST at RT, and visualized using EZ-ECL Chemiluminescence (Biological Industries, 20500-120) by ImageQuant™ LAS 4000 (GE Healthcare Life Sciences). Densitometric analysis was performed using ImageJ (NIH).
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bioRxiv - Microbiology 2022Quote: ... and imaged using the Amersham Imager 600 (GE Healthcare Biosciences) or the Biorad ChemiDoc™ Touch ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were developed with Amersham ECL (RPN2232, Cytiva) or Amersham ECL Prime (RPN2236, Cytiva) chemiluminescent western blot detection reagents and exposed using Amersham Hyperfilm ECL (GE Healthcare). Densitometry of scanned films was performed using ImageJ (Schneider et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-treated samples were next incubated with Protein A-Sepharose beads (GE Healthcare) at 4 °C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... elution from the HisPur™ Cobalt Resin was applied to a 5 ml streptactin column (GE Healthcare) and washed with HBS ...
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bioRxiv - Biochemistry 2022Quote: ... including a reference channel for online background subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface (Biacore T100 Control Software; GE Healthcare). All measurements were performed with 2-fold serial dilutions using 60 s association followed by a dissociation time of more than 240 s at 25 °C with a flow rate of at 30 μl min−1 ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibodies were anti-rabbit IgG (GE Healthcare, NA9340) and anti-mouse IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-mouse IgG (GE Healthcare, NA9310). Membrane was soaked with an ImmunoStar Zeta reagent (FujiFilm Wako Pure Chemical) ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was first purified by affinity chromatography using Glutathione Sepharose 4B resin (GE Healthcare) and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Measurement of titrations at equilibrium were used to determine Kd values using Biacore Analysis Software (v.2.0.4, GE Healthcare) and fits to a total binding model were performed in GraphPad Prism 9 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare) with SEC buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... were captured on an SA-chip (GE Healthcare), including a reference channel for online background subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface (Biacore T100 Control Software ...
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bioRxiv - Biochemistry 2022Quote: ... followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare) with SEC buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing GRK5 were combined and run on a Superdex 200 10/300 GL column (GE healthcare). GRK5 was aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Approximately 300-400 resonance units (RU) of FPLC-purified biotinylated arrestin in HBS-P+ Buffer (GE Healthcare) were captured on an SA-chip (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Biophysics 2022Quote: ... Delta RBD and Omicron RBD were immobilized onto Ni-NTA sensor chip (GE Healthcare) using a Biacore X100 (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The resulting data were fitted to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare). Also the ACE2 was immobilized onto CM5 chip and Serial dilutions of purified WT ...
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bioRxiv - Biophysics 2022Quote: ... The protein of interest was separated by affinity chromatography using resin attached with Ni-NTA and being subjected to additional purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 and 200 mM NaCl.
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bioRxiv - Biophysics 2022Quote: ... using a Biacore X100 (GE Healthcare). Serial dilutions of purified ACE2 were injected ranging in concentrations from 250 to 15.6 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were separated using Ficoll-Paque (GE Healthcare), and monocytes were isolated by positive selection using CD14+ magnetic beads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... and mononuclear cell isolation on Ficoll-Paque (GE Healthcare) density gradient ...
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bioRxiv - Neuroscience 2022Quote: ... 6.5 mg / run were loaded onto a Cytiva (formerly GE Life Sciences) 1 ml HiTrap Protein G HP antibody purification column (Cat # 29048581 ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound antibodies were detected with the Enhanced Chemiluminescence (ECL) system (GE Healthcare Amersham) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Glutathione Sepharose 4B beads (GE Healthcare) were washed and resuspended in binding buffer to prepare a 50% slurry ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed as previously described using Horseradish Peroxidase (HRP)-conjugated secondary antibody followed by addition of ECL (GE Healthcare Bio-Sciences Corp., USA) to reveal chemiluminescence71 ...