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Citations for Quantifoil Micro Tools :
1351 - 1400 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Freshly purified RS head core complex (0.4 mg/ml) was placed onto glow-discharged grids (Quantifoil R1.2/1.3 200 mesh Cu grids) and plunge-frozen into liquid ethane ...
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bioRxiv - Biophysics 2019Quote: 3.5 μl of sample of fragmented doublets (~4 mg/ml) or the Sarkosyl A-tubule (~500 μg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: 3.5 μl of microtubule fraction sample (∼4 mg/ml) purified from WT Chlamydomonas was applied to glow-discharged holey carbon grids (Quantifoil R2/2), blotted and vitrified in liquid ethane using the Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Biochemistry 2019Quote: ... 300 mesh grid (Quantifoil Micro Tools GmbH), and allowed to incubate for 1 minute at 4°C and 100% relative humidity ...
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bioRxiv - Biophysics 2019Quote: ... Cells were cultivated on Gold Quantifoil grids (R2/1, Au 200 mesh grid, Quantifoil Micro Tools, Jena, Germany). Additional carbon (20–25 nm ...
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bioRxiv - Biophysics 2019Quote: ... holey carbon copper EM grids (Quantifoil) and allowed to dry ...
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bioRxiv - Biophysics 2019Quote: ... 300-mesh Quantifoil R 1.2/1.3 grids (Quantifoil, Micro Tools GmbH). These grids were blotted for 3 seconds and subsequently plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 4°C and 90% humidity ...
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bioRxiv - Microbiology 2019Quote: 3 µl of S-layer suspension were applied to glow-discharged 300 mesh copper Quantifoil grids (R2/2, Quantifoil, Jena, Germany), blotted for 3 – 5 seconds and rapidly injected into liquid ethane using a homemade plunge-freezer ...
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bioRxiv - Biochemistry 2019Quote: ... The sample (3.5 µL) was applied to glow discharged UltrAUfoil 2/2 200 mesh gold grids (Quantifoil) and plunge-frozen using a vitrobot Mark IV (Thermo Fischer) ...
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bioRxiv - Biophysics 2019Quote: ... Cryo-EM grids (Quantifoil R2/1 Cu mesh 200-300 or Quantifoil R1.2/1.3 Cu mesh 300) were glow discharged with air plasma in a Cressington 208 carbon coater with glow discharge unit for 90 s (10 mA/0.1 mbar air pressure ...
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bioRxiv - Biophysics 2019Quote: ... Cryo-EM grids (Quantifoil R2/1 Cu mesh 200-300 or Quantifoil R1.2/1.3 Cu mesh 300 ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 ul of sample was applied to glow-discharged R2/2 gold grids (Quantifoil) which were blotted for 5 s and plunge-frozen in liquid ethane with a Vitrobot Mark IV (FEI ...
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bioRxiv - Biophysics 2019Quote: ... carbon-coated grid (Quantifoil R 1.2/1.3) inside a Vitrobot set at 6 °C and 100% humidity and incubated for 10 seconds before blotting and vitrifying in liquid ethane.
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bioRxiv - Biophysics 2019Quote: ... carbon-coated grid (Quantifoil R 1.2/1.3) inside a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... in the presence of ADPR and Ca2+ to freshly glow-discharged UltrAuFoil® R1.2/1.3 300-mesh grids (Quantifoil). The grids were blotted for 2 s at 25 °C under 95% humidity before being plunged into liquid ethane using a Leica EM GP2 (Leica Microsystems) ...
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bioRxiv - Biochemistry 2019Quote: ... a thin amorphous carbon film was floated onto UltrAuFoil® R1.2/1.3 300-mesh grids (Quantifoil). 3 μL of TRPM2 (0.5 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 70 nm Lowicryl sections generated as described above for correlative light and electron tomography were cut using Leica UltraCut UC7 onto 200 mesh copper grids (Quantifoil Micro Tools GmbH). Immunolabeling was carried out with the MAb414 antibody diluted 1:100 in 1% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... Cryo-EM grids were prepared by application of 3.5 µl of the sample containing purified Ccr4-Not:ribosome complexes onto R3/3 copper grids with 3 nm continuous carbon support (Quantifoil) and vitrification in liquid ethane using a Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Plant Biology 2019Quote: ... 4 µL of mat3-4 cell culture was blotted onto R2/1 carbon-coated 200-mesh copper EM grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Microbiology 2019Quote: Vitrified specimens were prepared by adding a 3.5 µl drop of the different samples to holey grids (TedPella, Quantifoil grids, Copper, 300 mesh, R1.2/1.3), which had been overlaid with an additional thin layer of carbon ...
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bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
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bioRxiv - Biochemistry 2019Quote: ... Briefly: Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools, Jena, Germany) were washed overnight in chloroform (Sigma Aldrich ...
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bioRxiv - Biophysics 2019Quote: An aliquot of 3.5 μL GCGR-Gs-Nb35 complex was applied to glow-discharged 200 mesh grids (Quantifoil R1.2/1.3), at a concentration of 17 mg/ml and subsequently vitrified using a Vitrobot Mark IV (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2019Quote: ... This sample was double blotted (3.5 μl per blot) onto 200 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) at 4°C and 100% humidity and plunge frozen in liquid ethane cooled to the temperature of liquid nitrogen (FEI Vitrobot) ...
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bioRxiv - Biophysics 2019Quote: ... This sample was double blotted (3.5 μl per blot) onto 200 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) at 4°C and 100% humidity and plunge frozen in liquid ethane cooled to the temperature of liquid nitrogen (FEI Vitrobot) ...
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bioRxiv - Cell Biology 2019Quote: Continuous carbon grids (200-400 mesh copper, Quantifoil) were glow-discharged (PELCO EasiGlow ...
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bioRxiv - Biochemistry 2019Quote: ... 3 μl were applied onto freshly glow-discharged Quantifoil Cu/Rh R2/2 holey carbon 200 mesh grids (Quantifoil). The grids were blotted for 4 seconds with a blotting force of −15 ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μl 60 μM H6-TtBac in Buffer C were added onto freshly glow-discharged Quantifoil Cu/Rh R2/2 holey carbon 200 mesh grids (Quantifoil). The grids were blotted for 2.5 s with a blotting force of −15 ...
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bioRxiv - Cell Biology 2019Quote: ... R1/4 or R1/20 (Quantifoil Micro Tools, Jena, Germany) were employed in this study ...
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bioRxiv - Microbiology 2019Quote: ... 3.5 μL of unstained nanoparticles were applied to glow discharged 200 mesh R2/2 Quantifoil Cu grids (Quantifoil, Großlöbichau, Germany) and plunge frozen using a Vitrobot Mark IV plunger (FEI ...
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bioRxiv - Biophysics 2019Quote: ... We then used a glow-discharged Quantifoil EM grids (R2/1, Cu 300 mesh; QUANTIFOIL Company) to gently touch the powder for several times ...
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bioRxiv - Biophysics 2019Quote: ... of suspended crystals was loaded on one side of glow-discharged Quantifoil EM grids (R2/2, Cu 300 mesh; QUANTIFOIL Company). The grid was then blotted from the other side and washed twice manually with 4 μL 5% v/v PEG 200 buffer ...
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bioRxiv - Immunology 2019Quote: 4 μL of C5-OmCI-RaCI-CirpT1 in PBS (0.3 mg/ml) was applied to freshly glow-discharged (20 s, 15 mA) carbon-coated 200-mesh Cu grids (Quantifoil, R1.2/1.3). Following incubation for 10 s ...
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bioRxiv - Molecular Biology 2019Quote: ... About one microliter of a crystallization drop and two microliters of reservoir solution were applied onto a holey carbon-coated grid (Quantifoil R1.2/1.3, Quantifoil Micro Tools GmbH) and the grid was blotted manually with filter paper and frozen in liquid nitrogen.
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bioRxiv - Molecular Biology 2019Quote: ... About one microliter of a crystallization drop and two microliters of reservoir solution were applied onto a holey carbon-coated grid (Quantifoil R1.2/1.3, Quantifoil Micro Tools GmbH) and the grid was blotted manually with filter paper and frozen in liquid nitrogen.
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bioRxiv - Microbiology 2019Quote: 3 μl of purified complex at 1 to 3.6 mg/ml were applied to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3). Grids were blotted for 3 s at 100% humidity at 22 °C and frozen in liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2019Quote: ... holey carbon R1.2/1.3 grids with gold 400 mesh (Quantifoil Micro Tools) were glow discharged at 15 mA for 30 s (PELCO easiGlow Glow Discharge Cleaning System) ...
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bioRxiv - Cell Biology 2019Quote: ... R 2/1 or R 3.5/1 on 300 mesh Cu/Rh grids (QUANTIFOIL) were glow discharged for 45 s at 25 mA ...
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bioRxiv - Molecular Biology 2019Quote: The concentration of the core BBSome as derived from chemical crosslinking was adjusted to 0.05-0.08 mg/ml and 4 μl of sample was immediately applied to a glow-discharged UltrAuFoil® R1.2/1.3 holey gold grid (Quantifoil). After two minutes incubation at 13 °C and 100% relative humidity (RH) ...
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bioRxiv - Biophysics 2019Quote: ... Perforated carbon film-covered microscopical 200 mesh grids (R1/4 batch of Quantifoil, MicroTools GmbH, Jena, Germany) were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems ...
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bioRxiv - Microbiology 2019Quote: ... The EM-grids were glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). 3,5 µl of the sample was applied to the EM grid and blotted at a temperature of 16°C and a humidity between 95-99% ...
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bioRxiv - Pathology 2019Quote: ... of the purified MNV infectious particles and HNV GII.3 VLP were placed onto R 1.2/1.3 Quantifoil grids (Quantifoil Micro Tools) coated with a thin carbon membrane that were glow-discharged using a plasma ion bombarder (PIB-10 ...
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bioRxiv - Biochemistry 2019Quote: ... Samples of the freshly prepared GFLV-Nb23 complex (2.5 µl of a 0.5 mg/mL solution) were applied to 300 mesh holey carbon Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools, Jena, Germany), blotted with filter paper from both sides in the temperature- and humidity-controlled Vitrobot apparatus (FEI ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.4 μl of 0.12 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.8 µl of 0.10 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
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bioRxiv - Biochemistry 2019Quote: ... applied to 300-mesh carbon coated (2nm thickness) holey carbon Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) and flash-frozen as described in ref (22) ...
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bioRxiv - Biophysics 2019Quote: ... The Netherlands) as fiducial markers and glow-discharged R2/2 Cu 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) were dipped into the solution ...
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bioRxiv - Biophysics 2019Quote: Quantifoil R1.2/1.3 Au300 and UltraAufoil R1.2/1.3 Au300 grids (Quantifoil Micro Tools, Jena, Germany) were used as sample carriers ...
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bioRxiv - Microbiology 2019Quote: Cells of strain Pla133T were mixed with the same volume of BSA-stabilized 15 nm colloidal gold solution (Aurion) and placed on holey carbon-coated 200 mesh copper grids (R2/1, Quantifoil, Jena, Germany) immediately before thin-film vitrification by plunge-freezing in liquid propane (63%)/ethane (37% ...
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bioRxiv - Microbiology 2019Quote: ... 5 µl of purified pelleted EVs in HBSS were applied to carbon-coated copper grids (R1.2/1.3, Quantifoil Micro Tools GmbH) and the excess of liquid was blotted automatically for two seconds from the reverse side of the grid with a strip of filter paper ...