Labshake search
Citations for Quantifoil Micro Tools :
1251 - 1300 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... or Quantifoil holy carbon grids (R1.2/1.3, 400 mesh gold, Quantifoil Micro Tools). The grids were blotted for 7.5 s with a blot force of 16 and 100% humidity before being plunged into liquid ethane cooled by liquid nitrogen.
-
bioRxiv - Molecular Biology 2020Quote: ... of 0.5 mg/mL purified SARS-CoV-2 S protein were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R1.2/1.3, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... and 3 µl was immediately loaded onto plasma cleaned Quantifoil R 2/1 holey carbon copper gri (Cu, 400-mesh, Quantifoil Micro Tools GmbH). Blotted grids were plunge-frozen into nitrogen-cooled liquid ethane.
-
bioRxiv - Immunology 2020Quote: ... four-microliter aliquots of Fcα-J-SC (0.3 mg/ml) or Fcα-J-SC-SpsANTD (0.2 mg/ml) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3), blotted with filter paper at 4 °C and 100% humidity ...
-
bioRxiv - Biochemistry 2020Quote: ... UltrAuFoil R1.2/1.3 300 mesh gold grids (Quantifoil) were rendered hydrophilic using the Tergeo plasma cleaner (Pie Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was applied onto a holey carbon grid (Quantifoil, Cu, R1.2/1.3, 300 mesh) which was rendered hydrophilic by a 30 s glow-discharge in air (11 mA current ...
-
bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
-
bioRxiv - Biophysics 2020Quote: ... 2.5 μl samples of GDN-purified proteins at a concentration of 1.5-3 mg ml−1 were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 or R0.6/1 Au 200 mesh). Excess liquid was removed in a controlled environment (4°C and 100% relative humidity ...
-
bioRxiv - Biophysics 2020Quote: The purified N protein was prepared on UltraAuFoil R1.2/1.3 gold support grids (Quantifoil). 3 µl of sample was applied to glow-discharged grids ...
-
bioRxiv - Cell Biology 2020Quote: ... 2.5 μl of samples was frozen onto a glow discharged Quantifoil Gold R 1.2/1.3 300 mesh grid (Quantifoil). Samples were frozen under 100% humidity ...
-
bioRxiv - Molecular Biology 2020Quote: A 3 μL sample solution was applied to a Quantifoil holey carbon grid R1.2/1.3 Mo 200 mesh (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Biophysics 2020Quote: ... then positioned on a holey carbon TEM grid (Quantifoil R2/1, Cu, 200-mesh) pre-clipped into an Autogrid assembly (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... 3.5 uL of sample was applied at a concentration of 3-5 mg/mL to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: Quantifoil R2/2 holey carbon copper grids (Quantifoil Micro Tool) were prepared by first applying an additional thin layer of continuous carbon and then glow-discharging them for 15 sec at 15 mA using an easiGlow Discharge cleaning system (PELCO) ...
-
Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
-
Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
-
bioRxiv - Biochemistry 2020Quote: The cryo-EM grids were prepared by applying 3 μL of the human MCU-EMRE-MICU1-MICU2 complex in nanodisc (Ca2+-free or Ca2+-bound state) to a glow-discharged Quantifoil R1.2-1.3 300-mesh gold holey carbon grid (Quantifoil, Micro Tools GmbH, Germany) and blotted for 4.0 seconds under 100% humidity at 4 °C before being plunged into liquid ethane using a Mark IV Vitrobot (FEI) ...
-
bioRxiv - Biochemistry 2020Quote: ... holey carbon grid (Quantifoil R1.2/1.3, Cu, 400 mesh), blotted by Vitrobot Mark IV (FEI ...
-
bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
-
bioRxiv - Molecular Biology 2020Quote: ... plain grids (Quantifoil 2/2) were transferred to the microscope ...
-
bioRxiv - Biophysics 2020Quote: ... An aliquot of 4 μl pY39 α-syn fibril solution was applied to a glow-discharged carbon electron microscope grid (Quantifoil R1.2/1.3, 300 mesh). After 10 s of incubation ...
-
bioRxiv - Biophysics 2020Quote: Cryo-EM grids were prepared by applying freshly purified channel protein (~3.5 μl) to glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil), which were then blotted for 2 s with ~100% humidity and flash frozen in liquid ethane using FEI Vitrobot Mark IV (FEI) ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or Cu 200 Holey Carbon R2/1 grids (Quantifoil, for samples containing 1.5 mM FFosC). In both cases ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil, for samples without FFosC), or Cu 200 Holey Carbon R2/1 grids (Quantifoil ...
-
bioRxiv - Neuroscience 2020Quote: ... holey carbon copper EM grids (Quantifoil) and allowed to dry ...
-
bioRxiv - Molecular Biology 2020Quote: ... 400-mesh Quantifoil 1.2/1.3 Cu grids (Quantifoil, Großlöbichau, Germany) were made hydrophilic by glow discharging for 60 seconds with a current of 15 mA in a Pelico EasiGlow system ...
-
bioRxiv - Molecular Biology 2020Quote: ... transferred onto allgold UltrAuFoil R0.6/1 grids (QuantiFoil), and partially hydrogenated following a previously described procedure [17] ...
-
bioRxiv - Molecular Biology 2020Quote: QUANTIFOIL R 2/1 grids with the 2-nm carbon layer (Cu 200, Quantifoil Micro Tools) were glow discharged with 25 mA with negative polarity for 60 s in a PELCO easiGlow glow discharge unit ...
-
bioRxiv - Molecular Biology 2020Quote: Samples were diluted to a concentration of 60-65 nM for the Pol II-U1 snRNP complex and 2 μl was applied to each side of the R2/2 UltrAuFoil grids (Quantifoil) that had been glow-discharged for 100 s ...
-
bioRxiv - Molecular Biology 2020Quote: ... a 2.2-µL aliquot of this sample was applied to a plasma cleaned holey carbon grid (R2/1, 200 mesh; Quantifoil) or Graphene Oxide-Lacey Carbon grid (300 mesh ...
-
bioRxiv - Neuroscience 2020Quote: Samples for negative stain transmission electron microscopy were placed on 200 mesh formvar-carbonated copper grids with (Plano, Quantifoil #S162). Grids were glow-discharged for 30 s (Baltec ...
-
bioRxiv - Neuroscience 2020Quote: ... covered with either 2 nm amorphous carbon (Quantifoil, R2/1+2 nm C) or graphene were used for sample preparation ...
-
bioRxiv - Neuroscience 2020Quote: Holey carbon grids (Quantifoil R2/1) covered with either 2 nm amorphous carbon (Quantifoil ...
-
bioRxiv - Biophysics 2020Quote: 0.8 mg/mL aldolase solution was applied to glow-discharged gold holey carbon 1.2/1.3 200 mesh grids (Quantifoil). Grids were blotted for 3 seconds at 0 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
-
bioRxiv - Biophysics 2020Quote: ... 2 μl apoferritin was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil). Grids were blotted for 4 seconds at -2 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
-
bioRxiv - Biophysics 2020Quote: ... all apoferritin and aldolase samples were frozen on 300-mesh Quantifoil gold holey carbon grids with hole size and spacing of 1.2/1.3 μm (Quantifoil) using a Mark IV vitrobot (ThermoFisher) ...
-
bioRxiv - Biochemistry 2020Quote: An aliquot of 3 μL of SAM complex sample was applied to freshly glow-discharged holey carbon grid (Quantifoil R1.2/1.3, copper, 300 mesh). The grids were blotted for 6 s and plunge-frozen in liquid nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV plunger ...
-
bioRxiv - Biophysics 2020Quote: ... The protein was then blotted onto Cu 300 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) two times with 3.5 μl sample each time ...
-
bioRxiv - Microbiology 2020Quote: ... 3,5 μl of the culture was mixed with 10 nm gold fiducial markers (Cell Microscopy Core, UMC Utrecht) and applied onto glow-discharged R2/2 200 mesh holey carbon EM grids (Quantifoil). The grid was then rapidly plunged in liquid ethane using the Leica GP automated freeze-plunger ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
-
bioRxiv - Microbiology 2020Quote: Holey carbon-coated (R 2/2; Quantifoil) Au-G200F1 grids were analyzed in cryo-conditions by MISTRAL microscope at ALBA synchrotron ...
-
bioRxiv - Microbiology 2020Quote: ... 105 fluorescence-stained cells were deposited on the surface of Au-G200F1 finder grids coated with holey carbon (R 2/2; Quantifoil) and functionalized with Poly-L-Lysine (Merck ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 μL of eluate (72 nM) was applied to a plasma-treated UltraAuFoil 1.2/1.3 grid (Quantifoil, Großlöbichau, Germany). The grids were hand blotted for 3 sec from the backside of the grid before being plunged into liquefied ethane.
-
bioRxiv - Cell Biology 2020Quote: ... Features used for alignment purposes included both the nanoparticles added on samples just before vitrification as well as grid patterns (grid bars, Quantifoil holes and imperfections) and cellular features that displayed good contrast in both X-ray and light microscopy (fluorescent endosomes in this case).
-
bioRxiv - Cell Biology 2020Quote: U209 cells stably expressing mCherry-Gal3 were seeded onto TEM grids (Quantifoil AU G200F1 finder) 16 hours prior to infection ...
-
bioRxiv - Molecular Biology 2020Quote: 3.5 µl of the concentrated sample were applied to glow discharged copper grids with holey carbon support and a 2 nm continuous carbon layer (R3/3, Quantifoil). Grids were blotted in a Vitrobot Mark IV (FEI Company ...
-
bioRxiv - Molecular Biology 2020Quote: ... UltrAuFoil R1.2/1.3 300 mesh grids (Quantifoil GmbH) grids were glow-discharged for 30 seconds at 25 mAmp and used within 15 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 μl of purified PA7LF3 at a concentration of ∼0.06 mg/ml was applied onto freshly glow discharged grids (Quantifoil R 1.2/1.3 holey carbon with a 2 nm additional carbon support ...