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Citations for Millipore Sigma :
4701 - 4750 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Other antibodies used in this study include those specific to human CD3 (UCHT1 clone, Cat. number 217570, Merk-Millipore), Vav1 (Cat ...
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bioRxiv - Microbiology 2019Quote: ... a human telomerase immortalised retinal pigment epithelial line was passaged in Dulbecco’s modified minimal essential medium (DMEM)-F12 (Sigma) supplemented with 200 mM glutamine ...
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bioRxiv - Immunology 2019Quote: ... monocytes were cultured at 1 × 106 cells/ml in RPMI-1640 medium supplemented with 1% human AB serum (Sigma), 20 ng/ml M-CSF (Peprotech) ...
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bioRxiv - Molecular Biology 2020Quote: ... Human HEK-293T cells were grown in Dulbecco’s Modified Eagle’s high glucose medium with HEPES (Sigma-Aldrich®, D6171) supplemented with 10% fetal bovine serum ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... an additional blocking step was performed by incubating cells with 2 mg/ml γ-globulins from human blood (Sigma) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were washed with 60x slurry volume in lysis buffer and eluted through overnight incubation at 4°C with 160μl of Human Rhinovirus (HRV)-3C protease (Millipore) in a final volume of 2-3ml ...
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bioRxiv - Bioengineering 2020Quote: ... Three sections at varying depths within the tumor are immunostained with mouse anti-human nuclei (clone 235-1, Millipore) followed by secondary Dylight 488 horse anti-mouse (Vector) ...
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bioRxiv - Synthetic Biology 2021Quote: ... IPSCs were plated at 25,000 cells/cm2 in a 24-well plate coated with 15µg/ml human laminin (Sigma, USA). The following day ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded onto glass coverslips coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation) at a density of 80,000 cells and 40,000 cells for the PDGFRα homodimer cell line and PDGFRβ homodimer cell line ...
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bioRxiv - Cancer Biology 2020Quote: Human AGCT-derived KGN cells (Riken, Tsukuba, Japan) and JGCT-derived COV434 cells (Sigma-Aldrich, St. Louis, MO, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)-Ham’s F12 (Caisson ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human neuroblastoma cells (SH-SY5Y) were obtained from the European Collection of Cell Cultures (ECACC, Sigma-Aldrich, Dorset, UK) and grown in a 1:1 minimal essential medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... The activated fluidic channels were coated with 0.5 mg/mL collagen type IV human placenta (Sigma Aldrich, C5533-5MG) in the cell culture incubator overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature ...
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bioRxiv - Genetics 2021Quote: Human embryonic kidney (Hek) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Biochemistry 2020Quote: ... adherent human embryonic kidney (HEK) 293T cells were transfected with the expression plasmids with branched polyehtyleneimine (PEI, Sigma-Aldrich). In detail ...
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bioRxiv - Cell Biology 2021Quote: ... Human cells were trypsinized and incubated in cold PBS supplemented with 5% fetal calf serum (Sigma-Aldrich; PBS-FACS). DNA was stained either by propidium iodide (PI) ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30 μg/mL fibronectin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and the cleavage fragments of C3b were detected by immunoblotting with a polyclonal anti-human C3b antibody (HPA020432; Sigma) (1:1000) ...
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bioRxiv - Cancer Biology 2020Quote: ... The human glioma initiating cells (GS7-11, GS8-11, GSC280) were maintained in serum-free DMEM/F12 medium (Sigma), containing B27 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Black Falcon clear flat bottom 96-well plates were coated with 5 μg/mL human collagen I (Sigma-Aldrich) in 2 mM acetic acid (Merck) ...
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bioRxiv - Cancer Biology 2020Quote: Glioma cells were grown as attached on cell-treated vessels coated with 10ug/mL human plasma fibronectin (Millipore, FC010) and transfected with X2 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 6μl of 10μM siRNAs targeting human KLC1 or KLC2 were mixed with 12μl HiPerFect in high-glucose DMEM medium (Sigma-Aldrich) and combined with 1×105 cells per well of a 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... and MDA-MB-231 (triple-negative human breast adenocarcinoma) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium with HEPES modification; Sigma, D1152) supplemented with 10 % fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney epithelium 293T (HEK293T) cells and Hela cells were cultured in DMEM-high glucose (Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at a density of 4×104 to 5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12-well plate in GK15 medium (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBS was replaced with a solution of TCNB buffer (50mM Tris, pH7.5, with 100mM NaCl, 10mM CaCl2) containing 89.5 nM human MMP9 (Sigma SAE0078) and incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Microbiology 2021Quote: ... THP-1 (ATCC® TIB-202™) human monocyte cell line was cultured in RPMI-1640 medium (Sigma, R8758) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human adenocarcinoma alveolar basal epithelial cells (A549, ATCC) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: Human colorectal adenocarcinoma cell lines HT29 and HCT116 (both European Collection of Cell Cultures through Sigma-Aldrich, Dorset, UK) were grown in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biochemistry 2021Quote: The hnRNP L_RRM2 fragment (residues189-286) was amplified from the human brain cDNA library and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (termed p28a) ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... digested splenic cells were then cultured in MEM-α with 5% human platelet lysates (PLTMax; Cat# SCM142, Millipore Sigma) at a density of 2-5 × 104 cells/mL in culture flasks for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 50% Neurobasal Medium, 0.5% N2 supplement, 1% B27 without vitamin A [Life Technologies, 12587-010], 0.025% [v/v] human insulin [Sigma, I9278] ...
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bioRxiv - Cancer Biology 2022Quote: ... human tumor slides were stained by immunohistochemistry with an antibody against Tenascin-C (1:300, EMD Millipore, Cat. ab19011), a strain-sensitive ECM protein known to be present in LUAD [9 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 (human embryonic kidney, ATCC) and U2OS (osteosarcoma, ATCC) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium, Sigma-Aldrich) supplemented with 10% FBS and 2 mM L-glutamine.
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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bioRxiv - Pathology 2020Quote: ... the suspension adjusted to 1 × 106 parasites/mL in PBS and was incubated with 20% human complement (Sigma Aldrich). After 30 min ...
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bioRxiv - Systems Biology 2020Quote: ... Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K, Millipore). Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD ...
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bioRxiv - Developmental Biology 2020Quote: Cells were seeded onto glass coverslips coated with 5 μg/mL human plasma fibronectin purified protein (Millipore Sigma #FC010). At ~90–100% confluency ...
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bioRxiv - Cell Biology 2020Quote: Human 293T and mouse P19 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... The cGAS knockdown for RNA interference was achieved using Mission shRNA-encoding lentivirus directed to human cGAS mRNA (Sigma; GenBank/EMBL/DDBJ accession no ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Molecular Biology 2020Quote: Human FOP fibroblasts (Coriell Institute, NJ) were grown to confluence in DMEM supplemented with 10% FBS (Sigma-Aldrich, MO) and then seeded into 96-well plates (Costar® 3610 ...
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... at 14:30 and 48 hours later with 5 IU of human chorionic gonadotropin (5 IU, hCG; EMD Millipore). At the time of hCG injection ...