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Citations for Millipore Sigma :
4801 - 4850 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and AP-labeled goat anti-human IgA (α-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) was used for detection ...
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bioRxiv - Biochemistry 2022Quote: ... residues 353-598) and human RXRα (NR2B1) LBD (residues 223-462) that were inserted into a pET45b(+) plasmid (Novagen) as a TEV-cleavable N-terminal hexahistidine(6xHis)-tag fusion protein ...
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bioRxiv - Cancer Biology 2023Quote: Two individual pre-designed human PLXNB2 sgRNA CRISPR-Sanger clones and one non-targeting control vector (Sigma-Aldrich #0020) were ordered from Sigma as glycerol stock (Sigma-Aldrich #HS5000013567 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were extensively washed in 0.1M PB and TBS and blocked in 1% human serum albumin (HSA; Sigma-Aldrich) solution for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48h later by intraperitoneal injection of 5 IU human chorionic gonadotropin (PMS from SIGMA, HGC from Millipore USA). Mouse zygotes were obtained by mating B6/CBA stud males with superovulated B6/CBA females at a 1:1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... Membrane attack complex (MAC) binding was assessed by staining with rabbit anti-human C5b-C9 antibody (Catalog #204903, Sigma) at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903 ...
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bioRxiv - Bioengineering 2023Quote: ... All-trans Retinoic Acid (RA) (R2625) and Brain-derived neurotrophic factor (BDNF human) (B3795) were purchased from Sigma Aldrich.
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bioRxiv - Immunology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation using Hisopaque-1077 (Millipore) and SepMate PBMC isolation tubes (StemCell ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Human cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D6429) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼30% CD90+ stromal cells were dissociated at 15 days using human cardiac digestion buffer: 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphate buffered saline (PBS) containing 0.12% of DMSO and 4% of human serum albumin (HSA) (Sigma-Aldrich, MO, USA) was used as the negative control and incubation buffer ...
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bioRxiv - Immunology 2023Quote: Human Vγ9Vδ2 T cells were specifically expanded from fresh PBMCs ex vivo using 5 µM zoledronic acid (Sigma Aldrich) or 3 µM BrHPP (bromohydrin pyrophosphate ...
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bioRxiv - Microbiology 2023Quote: ... we added a calibration range by serially diluting a polyclonal IgG antibody from human sera solution (reference: I2511, Sigma), to reach concentrations ranging from 0.68 ng.mL-1 to 22 ng.mL-1 ...
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bioRxiv - Microbiology 2023Quote: ... Human liver hepatocellular carcinoma (HepG2) and Vero (African Green Monkey Kidney) cells (ATCC) were regularly maintained in DMEM (Sigma) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Cell Biology 2023Quote: ... human breast cancer (MCF7) and human embryonic kidney cells (HEK293T) cells were cultured in Dulbeco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Genetics 2023Quote: Human mCherry-tagged CTNS mRNA solutions were prepared in RNAse free water (500ng/µl) with 0.05% PhenolRed (Sigma, cat.no.P0290). All mRNA injections were performed at the one-cell stage using a FemtoJet microinjector (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... The degradation products of fibrinogen were detected by Western blot using rabbit anti-human fibrinogen polyclonal antibodies (Sigma-Aldrich) at a 1:5,000 dilution as described above.
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bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
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bioRxiv - Pathology 2023Quote: Human or mouse lung tissue slides (7 μm) were fixed in 3.7% formalin (Sigma-Aldrich, St. Louis, MA, USA), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Genomics 2023Quote: The human pre-B ALL cell line Reh (DSMZ, ACC 22) was maintained in RPMI-1640 medium (Sigma, R0883) supplemented with 2 mM L-Glutamine (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Human plasma like media (HPLM) was generated according to the published formulation59 with addition of 5% dialyzed FBS (Sigma) and Pen/Strep (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Immunology 2023Quote: Lentiviral vectors containing validated shRNA sequences targeting expression of human PRKCD (accession NM_006254) or a non-target control were obtained from Sigma (catalog numbers TRCN0000010193 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell were then fixed as previously described and all three batches were permeabilised with Triton X-100 0.5% in PBS and a Cy5-conjugated anti-human (AC112S, Sigma) was used for the detection of T-DM1 and T-DXd ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were seeded onto plasticware coated with a solution of 0.1 % gelatin containing 10 µg/ml human plasma fibronectin (FN) (Millipore). and collagen type 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human peripheral blood mononuclear cells (PBMCs) (FUJIFILM Wako Pure Chemical, Osaka, Japan) were cultured in RPMI1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Human mini-colons cultured in biological triplicates were extracted from the PDMS chips and digested with collagenase I (Sigma) at 100 U/ml concentration in Advanced DMEM/F12 with penicillin/streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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bioRxiv - Cell Biology 2024Quote: ... 3,3’ s-dihexyloxacarbocyanine iodine (DiOC6) and fibrinogen from human plasma were obtained from Sigma Aldrich (St. Louis, Missouri, USA). ML161 (PAR1 inhibitor ...
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bioRxiv - Genetics 2024Quote: ... cells were transferred into SFEM II supplemented with 3 U/mL hEPO (SCT) and 3% AB human serum (Sigma) for an additional 5 days of culture ...
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bioRxiv - Microbiology 2024Quote: Human neutrophils from the peripheral blood of healthy blood donors were isolated by density gradient centrifugation (Histopaque; Sigma–Aldrich) followed by hypotonic lysis of erythrocytes ...
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bioRxiv - Cell Biology 2024Quote: ... and human breast cancer cells (4175-LuT; Hoshino et al., 2015) were cultured in Dulbecco’s modified Eagle’s medium (Sigma‒ Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Human GBM commercial cell lines were maintained routinely in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, New York, NY, USA), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington, MA, USA, cat# SCC507) and cultured in vendor-specified homebrew media ...
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) and blocked in 1% human serum albumin (Sigma-Aldrich) in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... and glucagon were measured using a customized MILLIPLEX human metabolic Hormone Panel V3 (Millipore Sigma, cat. no. HMH3-34K). CCL2 ...
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bioRxiv - Cell Biology 2024Quote: Madin-Darby canine kidney cells with stable expression of human α-2,6-sialyltransferase (MDCK-SIAT1) (Sigma, catalog #: 05071502-1VL), human lung adenocarcinoma epithelial A549-Dual cells (InvivoGen ...
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bioRxiv - Cell Biology 2024Quote: ... human embryonic kidney HEK-293 T cells and human dermal fibroblasts (HDFs) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Microbiology 2024Quote: ... Chips were coated overnight at 4°C with ECM composed of 200 ug/mL of human Collagen IV (Sigma) + 100 ug/mL of Matrigel (Corning) ...
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bioRxiv - Genetics 2021Quote: ... then added 8 µL of X-gal (20 mg/mL in DMSO) and 5 µL of chloramphenicol (Sigma-Aldrich # C0378, 34 mg/mL dissolved in ethanol). After time intervals of 0 ...
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bioRxiv - Cancer Biology 2019Quote: ... 6 week-old mice received a single dose (8 μl g-1 body weight) of tamoxifen (25 mg ml-1 in corn oil, Sigma-Aldrich, St. Louis, MO, USA, T5648) by intraperitoneal injection ...