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Citations for Millipore Sigma :
4851 - 4900 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: The method of multiple inductions of acute endotoxaemia comprises intraperitoneal administration of 8 consecutive injections (every 3 days) of LPS 10 mg/kg (Lipopolysaccharides from Escherichia coli 026:B6, Sigma-Aldrich®, St. Louis, MO, USA), in saline (NaCl 0,9%) ...
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bioRxiv - Cell Biology 2021Quote: ... 40,000 cells/well for COS-7 cells and 30,000 cells/well for MEFs (Wild-type or Drp1 KO) were seeded into poly-D-lysine-coated 8 well glass chamber (Sigma, P6407-5MG, Cellvis, C8-1-N). The next day ...
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bioRxiv - Cell Biology 2020Quote: Media (screen media) used for drug treatment was phenol-free DMEM supplemented with 0.2% BSA FFA-free (Sigma-Aldrich, St. Louis, USA, Catalog 9048-46-8). The 1280 compounds were aliquoted as 2 μL at 1 mM into 16x 96-well plates and stored at −20C ...
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bioRxiv - Biophysics 2021Quote: ... was flowed into the chamber (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore, 219261-100KU, 5100 U/mg), 0.29 mg/mL pyranose oxidase (from Coriolus sp. ...
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bioRxiv - Physiology 2020Quote: ... Cells were treated with muscle DAMPs (1 μg/ml) or LPS (100 ng/ml for 24 hours and Dex (Sigma-Aldrich, D4902, 1×10−8 M) was further directly added in the well for 6 hours ...
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bioRxiv - Biophysics 2021Quote: ... and an oxygen scavenging system45,46 (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore, 219261-100KU, 5100 U/mg), 0.29 mg/mL pyranose oxidase (from Coriolus sp. ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μl of 10x concentrated virus in Type 2 medium was added to the well (instead of unconditioned Type 2 medium) for 72 hours along with polybrene (Sigma, #H9268; final concentration = 8 µg/ml). After transduction with pDual_dsCas9_Venus ...
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bioRxiv - Neuroscience 2024Quote: Postnatal day 8 mice received an intraperitoneal (IP) injection 0.6 µg/g of lipopolysaccharide (LPS) from Escherichia coli O55:B5 (Sigma-Aldrich, St. Louis MO, Catalog no. L2880) diluted in sterile 0.9% sodium chloride solution (normal saline) ...
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bioRxiv - Plant Biology 2024Quote: ... 5.5 µL of enzyme solution (275 ng of Trypsin Gold, Promega V5280; and 8 units of Benzonase, Merck Millipore 70746; in 83.3 mM TEAB pH 8.5) was added to the 27 µL of residual column volume and incubated overnight at 37℃ ...
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Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerancebioRxiv - Plant Biology 2022Quote: ... 150 mg/L cefotaxime] containing 20 mg/L hygromycin B and incubated at 26 °C under conditions of 16-h light (150 μmol photons m−2 s−1) and 8-h dark until roots were established and leaves touched the Phytatray™ II lid (Sigma-Aldrich, St. Louis, MO, USA). Plantlets were then transferred to soil.
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bioRxiv - Biophysics 2023Quote: ... Y-27632 Rock-inhibitor is diluted in cell culture medium to a final concentration of 10 μM. Inhibition of myosin in NK92 cells (Fig. 4) is performed with blebbistatin (Sigma-Aldrich,cat. no. 856925-71-8). Blebbistatin is dissolved in DMSO at a stock concentration of 10 mM and mixed with cell culture medium to a final concentration of 3 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1143-1300 of human RTEL1 were amplified by PCR then subcloned between the EcoRI and XhoI restriction sites of pET28 (Novagen). RTEL1 proteins were expressed as 6-histidine-fusions in the E ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Pathology 2022Quote: ... biopsies of human xenograft harvested at the indicated time post infection were digested with 0.4 mg/ml Liberase TL (Sigma Aldrich) in CO2-independent medium for 60 min at 37°C with gentle agitation (100 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Metastases and micro-metastases were assessed using IHC with a human mitochondria antibody (Millipore MAB1273 Anti-Mitochondria clone 113-1).
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Bioengineering 2019Quote: ... and subsequently incubated overnight with the Notch ligand Delta-1 (courtesy of the Bernstein Lab, FHCRC) or human IgG (Sigma) to achieve oriented immobilization ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were plated on either tissue culture plastic (TCP) or hydrogel substrates coated with 200µg/ml human plasma fibronectin (Corning, NY, USA and Millipore, Germany) at a density of 5,000 to 15,000 cells/cm2.
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bioRxiv - Biochemistry 2019Quote: ... anti-actin (A4700) and anti-tubulin antibody (T6199) and goat anti-human IgG–HRP antibodies were obtained from Sigma-Aldrich, Dorset ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was harvested for cytokine detection using MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL fibronectin (Gibco ...
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bioRxiv - Immunology 2021Quote: Focus reduction neutralization test (FRNT) in the presence of increasing concentrations [volume/volume] of complement from normal human serum (Sigma), including 0 ...
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bioRxiv - Immunology 2021Quote: ... the following components were added: 2 mM L-Glutamine (Fisher), 10% heat-inactivated (56°C, 60 min) human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Microbiology 2020Quote: ... The porous membranes were coated on both sides with collagen type IV from human placenta (0.5 mg/mL in water; Sigma-Aldrich) at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were incubated at 30°C for 15 or 30 minutes in the presence of 750 nM DCLK1 and 0.617 μg human calpain 1 (Sigma, C6108), then analyzed by SDS-PAGE.
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Biophysics 2021Quote: PDIs (400 nM) were solubilized in PBS and then added to a solution containing 0.2 mM human insulin (Sigma-Aldrich), 2 mM EDTA and 325 μM DTT ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Immunology 2020Quote: ... from 1:50 to 1:51200 in PBS containing 2% skimmed milk were added followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... For analysis of human OL 3000-4000 cells were seeded on wells coated with 5-10 µg/ml mouse-laminin (#L2020, Sigma) and 0.025 % poly-L-lysine (#P4707 ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated with secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-human Fab IgG (H+L) (Sigma, A0293) diluted 1:10000 in blocking buffer for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Pathology 2021Quote: ... PBMC-granulocytes (105) and platelets (105) of mice or human samples from different treatments as per manufacture protocol (Sigma Aldrich, USA catalog no ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney (HEK) 293 cells were purchased from ATCC and HEK-293 PDE4A4 stable cell line established by Millipore. HEK-293 and HEK-293 PDE4A4 cells were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...