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Citations for Millipore Sigma :
4651 - 4700 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Human IAV strains were propagated in MDCK cells in the presence of 1 µg/ ml TPCK trypsin (Sigma) and harvested after 72 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were seeded at 8-8.5×106 cells in 75 cm² or 16.5×106 cells in 150 cm² culture flasks (TPP) coated with 0.25% fibronectin from human plasma (Sigma) and 1% extracellular matrix from Engelbreth-Holm-Swarm murine sarcoma (Sigma ...
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bioRxiv - Genomics 2023Quote: ... HUDEP-2 were maintained in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 330 μg/mL human holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma) ...
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bioRxiv - Pathology 2023Quote: Human FXa (American Diagnostica Inc. Stamford, CT, USA) and thrombin (Sigma-Aldrich Co., LLC., St. Louis, MO, USA) activities were measured at the enzyme concentrations of 0.18 U/mL and 0.12 U/mL ...
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bioRxiv - Immunology 2023Quote: ... during 5 minutes at RT followed by blocking with 10 μg/mL human γ-globulin (Sigma-Aldrich, USA) in TBS-0.5% blocking reagent (Roche ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed and 25 µL/well of alkaline phosphatase-conjugated goat anti-human IgG (Sigma-Aldrich, US) and IgA (Southern Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... was purchased from ATCC (TIB-202) and LX-2 human stellate cells were purchased from Sigma Aldrich (SCC064). LSECs were cultured in endothelial cell basal medium-2 (EBM-2 ...
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bioRxiv - Microbiology 2024Quote: ... Baby Hamster Kidney (BHK) and Human Embryonic Kidney (HEK) 293 were grown in Dulbecco’s modified eagle’s medium (DMEM; Sigma) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated in 48-well plates pre-treated with 50 mg/mL human fibronectin (FC010-10MG, Millipore) and incubated for 24 hr ...
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bioRxiv - Biophysics 2024Quote: ... For microfluidic surface endothelialization the channels were incubated overnight at 4°C with 10% human fibronectin (Sigma-Aldrich). HUVECs (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... To this purpose culture dishes/glass slides were first coated with fibronectin (Sigma-Aldrich, Darmstadt, Germany, human plasma) at 5 µg/cm2 concentration diluted in sterile PBS ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat #T7039, Sigma)25 for 72 h ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat # T7039, Sigma)10 for 72 h ...
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Immunology 2024Quote: ... purified human Tsen were pre-treated with or without the proteasomal inhibitor MG132 (10 µM; M7449; Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% penicillin/streptomycin supplemented with 10 ng/ml EGF (Epidermal growth factor human, Sigma-Aldrich, Cat#E9644) and FGF (Fibroblast growth factor basic human ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were transduced with lentivirus encoding the ABE reporter at varying dilutions (1:10, 1:100, 1:500, and 1:1000) in the presence of 8 μg/ml polybrene (Millipore Sigma Cat. #: TR-1003-G). Three days after transduction ...
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bioRxiv - Microbiology 2019Quote: ... Precipitated cells were resuspended in 200 µL of TE-buffer (10 mM Tris HCl, 1 mM EDTA, pH 8) containing lysozyme from chicken egg white (Sigma Aldrich, St. Louis, Missouri, USA) at a concentration of 15 mg mL-1 and incubated for 30 min at ambient temperature with regular mixing ...
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bioRxiv - Genetics 2019Quote: ... the final concentration of Dvvw dsRNA was brought to 1000 ng/μl and 20% Phenol Red (Cat #143-74-8; Sigma-Aldrich, St. Louis, MO, USA) added for coloration ...
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bioRxiv - Neuroscience 2020Quote: (+)-MK-801 hydrogen maleate (CAS Number: 77086-22-7) and D-amphetamine hemisulfate salt (CAS Number: 51-63-8) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All drugs were dissolved in water at the same conditions as the home tanks ...
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bioRxiv - Microbiology 2021Quote: ... Induced spores were harvested at 8 h after the dilution and were purified from the remaining vegetative cells with Histodenz (Sigma-Aldrich, St. Louis, Missouri, USA) density gradient centrifugation (18) ...
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bioRxiv - Cancer Biology 2022Quote: ... both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060)) in parallel ...
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bioRxiv - Plant Biology 2022Quote: ... genotypes were grown on MS medium supplied with or without the indicated concentration of 1-aminocyclopropane-1-carboxylic acid (ACC) (Sigma-Aldrich, CAS 22059-21-8).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Immunology 2019Quote: ... female BALB/c mice (8 weeks old) were sensitized on days 0 and 14 with 20 µg ovalbumin protein (OVA, grade VI, Sigma-Aldrich Chemie GmbH, Munich, Germany) emulsified in 2 mg alum (Imject™ Alum ...
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bioRxiv - Bioengineering 2020Quote: ... The TEV protease was buffer exchanged (50 mM Tris pH 8, 150 mM NaCl) and concentrated in Amicon ultra spin filters (Sigma-Aldrich, Saint Louis, MO, USA) (10.000 MWC ...
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bioRxiv - Pathology 2021Quote: ... were starved 6 hours from Zeitgeber 14 (ZT14) and refeed for 8 hours with the CD and 20% glucose (Sigma-Aldrich, Saint Louis, MO, USA) in the drinking water.
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bioRxiv - Pharmacology and Toxicology 2019Quote: The protein samples were separated by 8% and 12% SDS PAGE and were transferred to PVDF membranes (Merck Millipore, USA, Cat# ISEQ. 00010, LOT#R6PA4145H). The membranes w ere blocked with 5% skim milk for 3 h at 37 °C and were incubated for 14 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Immunology 2021Quote: ... the plate was washed and blocked with 2%BSA in BBT (Borate Buffer Saline pH 8.2, SIGMA Cat# 08059-100TAB-F, 8 mL Tween 20) for 1 hr/RT ...
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bioRxiv - Biophysics 2023Quote: ... We purchased indium tin oxide (ITO) coated-glass slides with dimensions of 25 mm × 25 mm and surface resistivity of 8-12 Ω/sq from Sigma-Aldrich (St. Louis, MO). Artist grade tracing paper (Jack Richeson & Co. ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were diluted to a final concentration of 2 mg/mL with Laemelli sample buffer (188 mM Tris-HCl, 218 mM SDS, 8% glycerol, and 500 μM Bromophenol Blue (B5525, Sigma-Aldrich, St. Louis, MO, USA). An equal amount of protein ...
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bioRxiv - Plant Biology 2023Quote: ... 150 mg/L cefotaxime] containing 20 mg/L hygromycin B and incubated at 26°oC under conditions of 16-h light (150 μmol photons m-2 s-1) and 8-h dark until roots were established and leaves touched the PhytatrayTM II lid (Sigma-Aldrich, St. Louis, MO, USA). Plantlets were then transferred to soil.
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bioRxiv - Plant Biology 2023Quote: ... Proteins were extracted by the addition of an equal volume of ice-cold Tris-buffered phenol pH 8 (Sigma-Aldrich Corp., St Louis, MO, USA). The sample was shaken for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: The cells were isolated after 5 days of treatment with MTH to induce erythroid differentiation by centrifugation at 1200 rpm for 8 min at room temperature and lysed in Tri-reagentTM (Sigma-Aldrich, St. Louis, Missouri, USA) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Head and neck cancer cell lines were incubated overnight with media containing lentiviruses supplemented with 8 µg/ml of polybrene (Millipore-Sigma, no. TR-1003-G). Transduced cells were selected with sequential treatment of puromycin (Biogems ...
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bioRxiv - Neuroscience 2023Quote: ... Aurora) upon delivery of an odor at three dilutions (no dilution, 1:8, and 1:160 in mineral oil, Sigma-Aldrich; 10 presentations per dilution) was recorded ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for transduction of 1E+05 HEK 293T cells in a 24-well plate along with 8 μg/mL polybrene (Sigma Cat No. TR-1003-G) in 500 μL of complete DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... brains were transferred to 0.1 M PBS containing 0.05% sodium azide (PBSA) (Sodium azide ReagentPlus®, S2002-5G, Sigma Aldrich, CAS number: 26628-22-8) and kept at 4◦C for long-term storage ...
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bioRxiv - Biophysics 2021Quote: ... Micropatterned coverslips were functionalized with a solution of 10 µg mL-1 FN from human plasma (Sigma Aldrich #F1056) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... solutions of 89Zr-labeled TDM1 (4 μCi/μg) were prepared in PBS (pH 7.5) containing 1% w/v human serum albumin (HSA, Sigma) and 0.1% w/v sodium azide (NaN3 ...
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bioRxiv - Immunology 2021Quote: ... that serves as support for the culture system was prepared seeding human dermal fibroblasts (200,000) re-suspended in a fibrin gel made of fibrinogen (Merck-Millipore), thrombin (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... TIFs (Telomerase immortalised fibroblasts29) and human embryonic kidney (HEK)293Ts cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (D5796, Sigma). All cell culture medium was supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 1×105 ESCs were plated on a 3.8 cm2 plate coated with human plasma fibronectin (Millipore, cat. no. FC010) and cultured in N2B27 medium supplemented with 12 ng/ml bFGF (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Immunology 2019Quote: ... and 5 μm sections were prepared and incubated with a mouse anti-human IgG4 mAb (clone MRQ-44; Sigma) or isotype control ...
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bioRxiv - Synthetic Biology 2019Quote: ... we grew the cells in plastic 48-well plates that were pretreated with 50 μg/mL human fibronectin (Millipore) for at least 10 min at 37 °C before cell plating (to improve cell adherence) ...
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bioRxiv - Cancer Biology 2019Quote: The AsPC-1 and Capan-2 human PC cell lines were obtained from Sigma-Aldrich (St. Louis, MO, USA) and Thermo Fisher (Waltham ...
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bioRxiv - Neuroscience 2019Quote: ... the eye cup was treated for ∼ 15 minutes with human plasmin (∼ 50 µg mL−1, Sigma or Haematologic Technologies) to aid vitreous removal.