Labshake search
Citations for Millipore Sigma :
4501 - 4550 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... Cell pellets were resuspended in PBS and mixed with the equal volume of lysis buffer (3 M NaCl, 100 mM Tris-HCl pH 7.5, 4 mM EDTA pH 8, 20 mM ß-mercaptoethanol, Sigma Protease Inhibitor Cocktail (Sigma)) ...
-
bioRxiv - Microbiology 2019Quote: ... fumigatus from environmental samples 2 g of each sample was suspended in 8 ml of sterile distilled water with 0.85% NaCl (SIGMA, Sigma-Aldrich Chemie Gmbh, Steinheim, Germany) plus 0.01% Tween 20 (SIGMA ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10× lentivirus concentrates were diluted in 1 ml of Opti-MEM containing 8 × 10−3 µg/ml of polybrene (Sigma Aldrich; St. Louis, MO) and incubated overnight at 37 °C ...
-
bioRxiv - Physiology 2020Quote: ... packed mouse RBCs in a 1:8 dilution of Milli-Q H2O (Milli-Q® Integral Water Purification System, EMD Millipore Corporation, Billerica, MA), followed by centrifugation at 15000 × g for 5 min in a Microfuge 16 Microcentrifuge ...
-
bioRxiv - Cancer Biology 2021Quote: ... resuspended in BMGF medium containing ~1000 ng(p24)/mL of lentiviral particles plus 8 μg/mL polybrene (Sigma-Aldrich, Catalog No. TR-1003-G), and plated in a 24-well plate ...
-
bioRxiv - Physiology 2020Quote: Wildtype lean and obese mice were randomly divided into treatment groups and dosed daily with 8 mg of ethanolamine-O-sulfate (EOS; Sigma-Aldrich, St. Louis, MO), vigabatrin (United States Pharmacopeia ...
-
bioRxiv - Cell Biology 2019Quote: ... and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck, Sigma-Aldrich, l’Isle d’Abeau, France). After three cycles of freezing – thawing ...
-
bioRxiv - Immunology 2021Quote: ... followed by 1 mL of the resuspended viral pellet and polybrene at a final concentration of 8 µg/mL (TR-1003, Sigma Aldrich, St. Louis, MO). After 24 hr ...
-
bioRxiv - Cell Biology 2020Quote: 8 well Nunc Lab-Tek Chambered Coverglass was coated with 1:100 dilution of fibronectin in DPBS (Millipore Sigma, Burlington, USA, Catalog #FC010-5MG) for 30 mins and then washed with DPBS (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... thirty mice with colitis were obtained and randomly assigned into three groups (n =8) including the control group (Control) without EA (EA, Sigma-Aldrich,CAS141-43-5) supplements and two treatment groups that were supplemented with 500μM EA (LowEA ...
-
bioRxiv - Bioengineering 2020Quote: A 15 wt% Pellethane© solution was obtained by dissolving 8 g of Pellethane© pellets in 45.3 g of tetrahydrofuran (THF, Sigma Aldrich, Steinheim, Germany) at 37°C for 8 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... reactions were prepared in 8-strip tubes with individually attached caps and covered with a drop of mineral oil (Sigma-Aldrich Company Ltd, UK). Amplifications were performed on Applied Biosystems® Veriti thermal cyclers (Life Technologies ...
-
bioRxiv - Immunology 2022Quote: ... cells were transduced by adding ~1-2 ml of RV supernatants supplemented with 50 μM 2-βME and 8 μg/ml polybrene (Millipore, Cat # TR-1003-G), followed by centrifugation for 90 min at 524g at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... and cervical spinal cord tissues (C3-C6) were isolated (n=5-8/treatment; 1 male and 1 female per litter) and sonicated in Tri-Reagent (Sigma, St. Louis, MO, USA) and stored at −80°C ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were grown in EMM or EMM with 0.2 g/L leucine to between 4 x 106 and 1.5 x 107 cells/ml and mounted in Ibidi chambers (8-well, 80827) coated with lectin (Sigma, L1395, at 50 µg/mL). Cells were imaged on a DeltaVision Elite microscope equipped with a PCO edge sCMOS camera ...
-
bioRxiv - Neuroscience 2023Quote: ... all media was removed from organoid wells and replaced with 100 µL Maturation media containing Syn-C4A-GFP or Syn-GFP with 8 µg/mL polybrene (EMD Millipore, TR-1003-G). The final virus concentration was 10 MOI ...
-
bioRxiv - Molecular Biology 2022Quote: ... After 48 hours of incubation at 37 °C + 5% CO2 the medium was removed by inversion and cell monolayer was fixed with 8% formalin solution (Sigma-Aldrich, catalog #A252549, USA) for one hour and 20 minutes and then revealed with crystal violet 0.04% (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
-
bioRxiv - Bioengineering 2023Quote: ... Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 µM) Ro 20-1724 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... mice were deprived of water for 4hrs and orally administered 200 ml of PBS containing 8 mg of FITC-dextran (4000 Da; FD4, Sigma Aldrich, Saint Louis, MO) per 20 g of body weight ...
-
bioRxiv - Cell Biology 2024Quote: U-2OS G3BP1/2 KO cells7 seeded in a 6-well plate were infected at a 60-70% confluency with lentivirus expressing mEGFP-GS-G3BP variants (G3BP1, G3BP2A, and G3BP2B, G3BP1V11A, G3BP2BV11A) and co-incubated with 8 µg/mL polybrene (Millipore Sigma, TR-1003-G). Cells were spun down at 1000xg ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the HEK293T cells were transduced with lentivirus encoding the dGFP reporter in the presence of 8 µg/ml polybrene (Millipore Sigma TR-1003-G). Three days after transduction ...
-
bioRxiv - Plant Biology 2023Quote: ... One-liter cultures were centrifuged and resuspended in Buffer A (50 mM Tris pH 8, 200 mM NaCl) with protease inhibitor cocktail (Sigma, St. Louis, MO, USA), DNase I (Sigma ...
-
bioRxiv - Genomics 2023Quote: ... The seeded cells were transduced with the viral library by supplementing their media with 8 μg/mL of polybrene (Sigma-Aldrich Inc # TR-1003) and adding a variety of viral volumes ranging from 0 μL to 50 μL prior to centrifugation at 1000 g for 2 hours at 33°C ...
-
bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
-
bioRxiv - Microbiology 2023Quote: ... HIV infections were established via spinoculation at 1100xg for 90 min at 30C in the presence of 8 µg/mL polybrene (Millipore Sigma #TR-1003-G) as previously described18 ...
-
bioRxiv - Neuroscience 2023Quote: ... striatal neurons were plated at a density of 50K per well onto 24 well plates or 20K per well onto 8 well chamber slides coated with poly-L-lysine (Sigma Aldrich, St. Louis, USA) (100 µg/ml) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... we added an equal volume of stop solution (8 M urea, 1 M Tris–HCl [pH 8.0], and a small amount of Blue Dextran [Sigma Chemical, St. Louis, MO, USA]) to stop the reactions ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then spun down at 7,000g for 8 minutes and resuspended in 100 μL secondary hybridization buffer (10% Formamide, 2X SSC, 10% Ficoll PM-400 (Sigma-Aldrich F5415-25 mL)) ...
-
bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... Head and neck cancer cell lines were incubated overnight with media containing lentiviruses supplemented with 8 µg/ml of polybrene (Millipore-Sigma, no. TR-1003-G). Transduced cells were selected with sequential treatment of puromycin (Biogems ...
-
bioRxiv - Cell Biology 2023Quote: Titration of lentiviral vectors was performed by transducing HOS cells (ATCC CRL-1543) with serially diluted vector preparations in the presence Polybrene (5-8 µg/mL; Millipore Sigma, Burlington, MA, USA). HOS cells were grown in DMEM (Corning ...
-
bioRxiv - Cancer Biology 2023Quote: Transduction of MIA PaCa-2 cells using lentiviruses was done in 1:1 ratio of viral supernatant to media and supplemented with 8 µg/ml Polybrene (Sigma-Aldrich #TR-1003-G). Following an overnight incubation ...
-
bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... We lysed each pellet in 1% SDS lysis buffer: 1% SDS in 50 mM Tris (pH 8) containing protease inhibitor cocktail (Sigma Aldrich, catalog no. P8849) and 1 mM PMSF ...
-
bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA, #IPVH00010). The membrane was incubated at 4°C overnight with the following primary antibodies ...
-
bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 20 mL NP buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8) with 100 µl 10X Protease Inhibitor Cocktail (Sigma-Aldrich, Merck, Darmstadt, Germany). Resuspended cells were filtered with Miracloth (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell cultures grown in 96 well plates were washed with PBS and incubated with a staining solution (5nM TMRM (Sigma-Aldrich #115532-50-8) and Hoechst were diluted in growth media and incubated for 45 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody bound to GP was then detected with anti-human antibody conjugated to HRP (EMD Millipore, Burlington, MA) and Ultra-TMB substrate (ThermoFisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... after which slides were washed in phosphate-buffered saline and stained against Human Nuclei (MAB4190, 1:250, Millipore) and Human specific Ki67 (MAB4383 ...
-
bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
-
bioRxiv - Cell Biology 2020Quote: Senescence in normal human fibroblasts was induced using five treatments with 30 μM tert-butyl hydroperoxide (tBHP, Sigma) for 5 consecutive days (1 h/day ...
-
bioRxiv - Immunology 2021Quote: ... were added to either tissue culture-treated plates coated with 10 μg/ml human plasma fibronectin (Sigma-Aldrich) or Corning® Costar® Ultra-Low Attachment plates (Sigma-Aldrich) ...
-
bioRxiv - Genomics 2020Quote: ... mice underwent the full superovulation procedure with an intraperitoneal injection of 10 IU human chronic gonadotropin (hCG, Sigma) 48 hours after PMSG administration ...
-
bioRxiv - Bioengineering 2019Quote: ... or standard 6-well tissue culture plastic dishes were incubated with human plasma fibronectin (Millipore Sigma, Catalog #FC010) at a concentration of 10 μg/ml diluted in PBS (3 ml/well of ECM protein solution added ...
-
bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: ... human HT-1080 fibrosarcoma cells (CCL-121; ATCC) were maintained using Eagle’s Minimum Essential Medium (EMEM; Millipore Sigma) supplemented with 2 mM L-glutamine and containing 10% (vol/vol ...
-
bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
-
bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no ...
-
bioRxiv - Biochemistry 2021Quote: ... reactions were then incubated in the presence of 50 μg/mL (~0.1 units) human salivary α-amylase (Sigma) or 1.5 M hydroxylamine for a further 60 min at 37°C ...