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Citations for Millipore Sigma :
4901 - 4950 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
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bioRxiv - Cell Biology 2021Quote: ... Collected supernatant and lysed pellet were separately incubated with 3.4 mg/mL p-nitrophenyl-N-acetyl-β-D-glucosaminide (NAG, Sigma) in citrate buffer (pH 4.5 ...
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bioRxiv - Genomics 2021Quote: ... were dissociated using trypsin and plated at a density of 2×105 cells per cm2 on plated coated with 0.1 mg/mL of Poly-D-Lysine (Sigma, P0899) and 2.5 μg/mL of laminin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated on 75-cm2 tissue culture flasks coated with poly-D-lysine (10µg/mL, Sigma Aldrich, cat#P9155) and incubated at 37 °C in 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2020Quote: ... 35-050-061) and plated at a density of 5.0×104 cells per well on poly-D-Lysine (Sigma, P6407) coated 24 well glass-bottom plates (Fisher ...
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bioRxiv - Physiology 2020Quote: ... sections were blocked in 5% donkey serum for 1h and incubated in goat anti-periostin antibody (1/400, ref AF2955 R&D) diluted in 5% donkey serum in PBS (D9663, Sigma) over-night at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HBV genotype D inoculum (subtype ayw) was prepared from HepAD38 [84] cell supernatant by polyethylene-glycol-MW-8000 (PEG8000, SIGMA) precipitation (8% final ...
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bioRxiv - Microbiology 2020Quote: Freshly isolated purified human neutrophils were seeded on 12 mm Poly-D-lysine-coated (0.01 % solution overnight; Sigma-Aldrich; P7405) coverslips at a concentration of 1 × 106 cells/ml (5 × 105 cells/mL per well ...
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bioRxiv - Neuroscience 2021Quote: ... we seeded 2.5 x 105 aNSCs or 2×105 qNSCs onto Poly-D-Lysine (50 ng/mL, Sigma-Aldrich, P6407) pre-treated (30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... cells were plated onto poly-d-lysine-coated 60 mm cell culture dishes and treated as described above with Ara-C (Sigma) at a final concentration of 5 µM for 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were maintained at 37 °C for 7 d to allow colony formation before stained with 0.5% crystal violet (Sigma-Aldrich) in absolute methanol ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Cell Biology 2021Quote: ... were first coated with a solution of poly-D-lysine (10 μg/ml in PBS, Sigma-Aldrich, A-003-M) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Grids and dishes for neuronal cultures were coated with poly-D-lysine (Sigma-Aldrich; 1 mg/ml in borate buffer) for 24 h and washed three times with water ...
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bioRxiv - Biochemistry 2022Quote: ... ∼1.0 mg of purified compound was dissolved in 300 µL of deuterated NMR solvent CDCl3 (99.96% atom % D, Sigma-Aldrich) and transferred to solvent-matched Shigemi tube for analysis ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted in elution buffer (similar to the 150 mM salt wash but supplemented with 2.5 mM d-Desthiobiotin (Sigma-Aldrich)) where the volume depended on the expression levels before harvesting ...
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bioRxiv - Developmental Biology 2022Quote: Singe cell suspensions were prepared from retinas that were dissected in ice-cold HBSS and then incubated with collagenase D (1mg/ml) and DNAse (0.1mg/ml) (Millipore Sigma) in DMEM/F12 supplemented with 5% FBS for 30 min at 37•C ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue and cells were incubated in 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D-6564, Sigma, 1:1000). Biotinylated-secondary antibodies were from Vector Labs ...
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bioRxiv - Pathology 2022Quote: ... minced in ice-cold dissection buffer (2mL per kidney, 1X HBSS, Thermofisher #14065056, 2,5mM HEPES-KOH Sigma Aldrich #H0527, 35mM D-Glucose, Sigma #G7528 ...
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bioRxiv - Cell Biology 2022Quote: Freshly isolated MuSCs were plated in wells that were pre-coated with poly-D-lysine (0.1 mg/mL, EMD Millipore) and ECM (25 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were typically passaged just once about two days before the intended day of use and plated on glass-bottomed dishes coated with poly-D-lysine (Sigma). The glial medium was replaced with neuronal plating medium (see below ...
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Microbiology 2022Quote: Sialidase activity was fluorometrically monitored using the substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-Neu5Ac; Sigma-Aldrich). Reactions were performed in a final volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
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bioRxiv - Biophysics 2022Quote: ... vimentin-knockout and desmin-knockin MEFs is performed with cytocha-lasin D (Cat. No. C8273; Sigma-Aldrich, St. Louis, MO). Cytochalasin D is dissolved in DMSO at a stock concentration of 20 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... while BM chimerism was analysed 20 weeks after transplantation. Chimerism was assessed by flow cytometry (using the antibodies detailed in TableS7.d. 7AAD (Sigma) was used for dead cell exclusion ...
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bioRxiv - Cell Biology 2022Quote: ... mice were fasted for 4 hours followed by gavaging of 2 g/kg (10 ml/kg) of D-glucose (Sigma) dissolved in distilled water ...
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bioRxiv - Neuroscience 2020Quote: Neurospheres were dissociated into single cells and 50.103 cells were plated onto 24-well Glass Slips (Millicell EZSLIDE, PEZGS0896) coated with 0.1mg/ml Poly-D-Lysine (Sigma-Aldrich), in culture medium without EGF/FGF2 for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Microbiology 2021Quote: The effects of depolymerizing microfilaments on endocytosis was determined by incubating the endothelial cells with 0.4 μM cytochalasin D (Sigma-Aldrich) for 45 min prior to infection ...
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bioRxiv - Microbiology 2020Quote: ... virus containing media was transferred to a new tube and the cell pellet was lysed in 500 μl of lysis buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1% n-dodecyl β-D-maltoside, 1X protease inhibitor (Sigma)) ...
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bioRxiv - Neuroscience 2021Quote: ... or glass coverslips in 24-well plates (for immunocytochemistry) coated with Poly-D-Lysine (5 μg/ml, PDL, A-003-E; Millipore) and laminin (1 mg/ml Sigma L2020) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in culture medium (1.5 brains in 10ml per flask) and grown in vented T75 flasks coated with poly-D-lysine (PDL; Sigma-Aldrich; 5µg/ml in water ...
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bioRxiv - Neuroscience 2021Quote: ... N2A cells were incubated with Cytochalasin-D for 15 minutes or with Nocodazole for 30min prior recordings (both from Sigma, dissolved in DMSO and both used at a final concentration of 1 μM) ...
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bioRxiv - Physiology 2020Quote: ... mice were either treated with vehicle (water) or approximately 1 mg/kg/d of dexamethasone (Sigma-Aldrich; catalog no. 2915) dissolved in their drinking water ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% (vol/vol) heat-inactivated FBS onto coverslips previously coated with 50 μg/mL poly-D-lysine (Sigma) overnight at 4°C and 25 μg/mL laminin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Cortical neurons were plated in 6-well plates on coverslips coated with 100μg ml-1 poly-D-lysine (Sigma- Aldrich) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... Half of the media was removed and replaced after 48h supplemented and with 4μM cytosine 1-β-d-arabinofuranoside (Ara-C; Sigma). Neurons were fed by replacing half the volume of spent media with fresh media without serum or Ara-C every week thereafter.
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bioRxiv - Neuroscience 2020Quote: ... Experiments required the preparation of 24-well plates coated with a 1mg/ml poly-D-lysine hydrobromide (Sigma-Aldrich, P0899) solution for two hours and washed off with distilled water before cell plating ...
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: HFF dishes in triplicate were treated with 2 x 106 tachyzoites that had been incubated with cytochalasin D (Sigma-Aldrich) or an equal addition of media by volume ...
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bioRxiv - Neuroscience 2022Quote: ... The suspension containing the SGNs was filtered through a 40-μm cell filter and planted on glass coverslips pretreated with 0.5 mg/ml poly D-lysine (Sigma-Aldrich) and 1 mg/ml laminin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 96-well plate in micro islands (35,000 cells/well) and maintained with dopamine neuron medium (DPM) containing 11.5 mM D-glucose (Sigma, G8769-100ML), 2 mM L-glutamine (Gibco ...
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bioRxiv - Genetics 2022Quote: ... Chitinase activity was measured by monitoring the hydrolysis of 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside (Sigma-Aldrich, M5639), a fluorogenic chitin substrate suitable for measuring endochitinase activity ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...