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Citations for Millipore Sigma :
4601 - 4650 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... K986P and V987P (S-2P) (70–72) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Plant Biology 2019Quote: ... were sourced from Nottingham Arabidopsis Stock Centre and homozygous mutants where confirmed through genotyping PCR on genomic DNA using REDTaq® ReadyMix™ (Sigma) following their protocol ...
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bioRxiv - Genomics 2019Quote: ... Real time PCR was carried out in a Stratagene MX3005P thermocycler using SYBR Green Jump Start Taq Ready Mix (Sigma Aldrich, USA) for quantifying amplification results ...
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bioRxiv - Microbiology 2019Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Genomics 2021Quote: ... we performed up to 8 nested PCR reactions in parallel with an input of 100-200ng per reaction using the KAPA2G Robust ready mix (Sigma-Aldrich, KK5702) in a volume of 50μL ...
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bioRxiv - Cancer Biology 2021Quote: ... Aforementioned single clone cultures were STR validated and screened for any mycoplasma contamination using PCR based Myco detection kit (Sigma, MP0035-1KT) before use ...
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bioRxiv - Cancer Biology 2021Quote: ... The real-time PCR analysis was also performed with the Complementary DNAs using JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO) and SYBR green nucleic acid stain (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... DNA amplicons and related donor template levels were evaluated by semiquantitative PCR and qPCR (using KAPA SYBR FAST qPCR Kits, Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2020Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). Chlamydia trachomatis strain L2/434 Bu was obtained from the laboratory of Dr ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem, cat# KK2602) or Expand Long Template PCR System (Sigma, cat# 11681842001). Primers used for each PCR reaction are listed in Table S4.
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bioRxiv - Immunology 2024Quote: ... Cell lines were kept at 37°C in 5% CO2 and routinely checked for mycoplasma contamination via LookOut Mycoplasma PCR detection kit (Sigma, Cat. MP0035).
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bioRxiv - Cancer Biology 2023Quote: ... a polymerase chain reaction (PCR)-based method was used to screen for mycoplasma once a month employing the LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) and JumpStart™ Taq DNA Polymerase (D9307 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Real-Time PCR reactions were performed in 20 µL mixtures containing 10 µL KAPA SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich, KK4605), 200 nM final concentration of species-specific rpoD forward and reverse primer and up to 3 µL of sample template ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were tested every two weeks for mycoplasma contamination either by in-house DAPI staining or LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... blots were hybridized with Digoxigenin (DIG)-labeled DNA probes (Supplementary Table 2) amplified by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich; 11636090910). The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Neuroscience 2024Quote: All cell cultures were tested for mycoplasma contamination once per month using LookOut® Mycoplasma PCR Detection Kit (Sigma, cat.no. MP0035-1KT).
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bioRxiv - Microbiology 2024Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Bioengineering 2021Quote: ... GCaMP6f-expressing HEK cells were seeded on a twelve-well cell culture plates with 18-mm glass coverslips coated with poly-D-lysine (PDL) (10 μg/μl; P6407, Sigma-Aldrich, St. Louis, Missouri, USA) for 1-2h ...
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bioRxiv - Immunology 2021Quote: ... according to manufacturer’s instructions and pipetted on to selective Lysogeny Broth (LB) agar plates (50 μg mL−1 kanamycin, 20 mM IPTG, 80 μg mL−1 X-gal, all Sigma Aldrich, St Louis, MO, USA) and incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... All subsequent liquid subcultures were derived from colonies isolated from these plates and were grown in Luria-Bertani (LB) broth (Oxoid, Hampshire, UK; Sigma-Aldrich, St Louis, MO, USA), Columbia broth (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... The media was then carefully removed from the 10 cm dish and the enriched myoblasts population was seeded in a 6 well-plate coated with Matrigel (Sigma-Aldrich, St. Louis, MO, USA) in plating media ...
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bioRxiv - Microbiology 2022Quote: ... we used the same approaches as described above and spotted the 5 μl of bacteria on LB agar plates containing 2% of Skim Milk Powder for microbiology (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany). Plates were incubated at 25°C and monitored for hemolytic and protease activities after 1 ...
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bioRxiv - Microbiology 2020Quote: Confluent cell monolayers grown in 96-well plates were pretreated with a range of concentrations of brefeldin A (BFA, EMD Millipore CAS 20350-15-6) or Golgicide A (GCA ...
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bioRxiv - Cancer Biology 2019Quote: ... 5×104 cells/ACM of either less-invasive HT29 or highly-invasive HCT116 cells were used and 240 μL of the collagen mix was added to a 96-well plate (Corning® Costar® through Sigma-Aldrich, Dorset, UK). The gel mix was polymerised at 37°C for 15 minutes (min) ...
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bioRxiv - Microbiology 2022Quote: ... 2×10⁴ spores/mL (in 100 µl) were added to a CytoOne® 96-well plate (StarLab, Brussels, Belgium) containing 1xRPMI-1640 medium (Sigma Aldrich, St. Louis, MO), 165 mM MOPS buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2020Quote: Disks of 1 cm in diameter were cut from Aclar® foil (thickness 198 μm; TedPella, 10501-10) and placed in sterile Corning ® 12-well plates (Sigma-Aldrich, CLS 3737). Dissociated cells were plated on the these disks (cells from 1 blastoderm /disk ...
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bioRxiv - Neuroscience 2019Quote: ... which was diluted in DMEM/F12 Nutrient Mixture medium at concentrations ranging from 10−14 M to 10−5 M in clear bottom 96-well plates (Sigma-Aldrich; Cat. No. CLS3603-48EA). Luminescence was measured over a 30 second period using a FLUOstar Omega Plate Reader (BMG LABTECH ...
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bioRxiv - Genomics 2021Quote: HEK293T cells (ATCC® CRL-3216™) were seeded at a density of 5×105 cells/well in a 6-well culture plate (Sigma Aldrich, cat # CLS3516). The following day ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panel 1 (EMD Millipore Corporation, Charles, MO), and analyzed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 12] Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panels 1 and 2 (EMD Millipore Corporation, Charles, MO), prepared and analyzed according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2021Quote: ... construction of the scFv library and panning were carried out as described in extensive detail [29] with the following changes: A Maxisorp 96-well microtiter plate (Nunc, Sigma-Aldrich, St. Louis, MO, USA) was coated with 5 µg/ml streptavidin (Sigma s0977 ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Immunology 2021Quote: ... the plate was washed and blocked with 2%BSA in BBT (Borate Buffer Saline pH 8.2, SIGMA Cat# 08059-100TAB-F, 8 mL Tween 20) for 1 hr/RT ...
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bioRxiv - Developmental Biology 2022Quote: NPCs cells were plated into Matrigel-coated 10mm2 plate and treated for 3 days with SAHA (20 nM, Sigma-Aldrich, in DMSO (0.05%, Sigma-Aldrich)) in culture media before performing western blot analysis ...
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bioRxiv - Genomics 2023Quote: ... neoformans parental strains or segregants was set up by picking a fungal colony from YPD agar plates (50g/L YPD broth powder [Sigma-Aldrich], 2% Agar [MP Biomedical]) and resuspending it in 3mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Radiolabeled steroids were extracted from the media with help of Ethyl acetate and Isooctane (1:1 v/v) and separated through Thin Layer Chromatography (TLC) on a Silica gel coated aluminum plate (Supelco® Analytics, Sigma Aldrich Chemie GmbH, Germany) [56] ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was plated at a density of 5 × 105 cells/mL on cell culture plates pre-coated with poly-L-Ornithine (Sigma-Aldrich, St. Louis, MO, USA) in Neurobasal medium [Neurobasal-A medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... FAM76B knockdown U937 cells were plated in 6-well plates (5×105 cells/well) and treated with 10 ng/ml PMA (Sigma-Aldrich, St. Louis, Missouri, USA) in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... 50µl of each parasite solution was added to a plate along with equal amounts of the following compounds at the listed final concentrations: 5mM 2-deoxyglucose (Sigma Aldrich, cat. no. D6134-5G) + 25mM glucose (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... After 30 minutes incubation at room temperature the plate was washed 4 times with PBST and alkaline phosphatase substrate was added (Sigma Aldrich, S0942; St. Louis, MO). The plate was analyzed using a plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... BMECs were sub-cultured onto plates or Transwells that were pre-coated with solution of Collagen IV (Sigma, C5533, 1 mg/mL in acetic acid)/fibronectin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cenocepacia in a 3:1 ratio on SOB plates containing 100 μg/mL of 2,6-diaminopimelic acid (DAP, Sigma-Aldrich, St. Louis, MO, United States) for 2 days at 37°C ...
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bioRxiv - Physiology 2024Quote: ... Samples were eluted directly in a MTP 384 Polished Steel target plate (Bruker Daltonics, Billerica, USA) and diluted (1:1, v/v) with α-cyano-4-hydroxycinnamic acid matrix (Sigma-Aldrich, Saint Louis, USA). Sample-matrix co-crystallization was carried out by the dried-droplet method 20 ...
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bioRxiv - Bioengineering 2024Quote: ... 200 µl of MPB was added to each well and the filter plate was put on a vacuum manifold with vacuum applied (Sigma-Aldrich, Supelco PlatePrep; −100 mBar) until all wells were drained ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for transduction of 1E+05 HEK 293T cells in a 24-well plate along with 8 μg/mL polybrene (Sigma Cat No. TR-1003-G) in 500 μL of complete DMEM ...