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Citations for Millipore Sigma :
4451 - 4500 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: The LINC00261 wild type cDNA was PCR-amplified from pENTR/D-TOPO-LINC00261 (gift from Leo Kurian) with KOD Xtreme™ DNA Hotstart Polymerase (Millipore). The resulting PCR product was inserted into pRRLSIN.cPPT.PGK-GFP.WPRE via its appended BshTI/SalI cloning sites ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified in 40 μL reactions with 15-18 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and 500 nM of each primer P1.3 and P2.1 (synthesized by IDT ...
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bioRxiv - Molecular Biology 2019Quote: ... an EcoRI restriction site was removed by performing a PCR on AT1.03 and AT1.03R122KR126K pDRF-GW using KOD polymerase (Merck-Millipore, Burlington, Massachusetts, USA) with the forward primer 5’-ATACTAGTGCTAGCTCTAGACTCGAGTATGGTGAG-3’ and reverse primer 5’-ATAGCGGCCGCTGATCAGCGGTTTAAACTTAAGC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20) ...
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bioRxiv - Pathology 2022Quote: ... Pf2-HA_OE and pf2-HA_KO ChIP and input control samples) was recovered from Proteinase K treated samples using the GenElute PCR purification kit (Sigma-Aldrich).
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bioRxiv - Plant Biology 2019Quote: ... Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen, Wisconsin, USA) and transformed into E ...
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Cancer Biology 2019Quote: ... digital PCR was carried out using a Biomark HD (Fluidigm) with a 65bp TaqMan assay for the housekeeping gene RPP30 (Sigma Aldrich) (7) ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... was tagged with 6xHis at the C-terminus using PCR amplification and the modified Tau-His cassette was introduced into pET3a plasmid (Novagen, DE) linearized with NdeI enzyme succeeding in pET3a/htau40/6xHis plasmid for the protein expression in E.coli ...
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Genomics 2020Quote: ... The numbers of amplification cycles needed for library preparation of each sample was then determined by quantitative PCR as follows: 25 µl SYBR Green Jumpstart Taq ReadyMic (Sigma-Aldrich), 5µl PCR product from previous reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... Gel-electrophoresis was used for the detection of PCR products (MYC 630 bp, Neu 386 bp, rtTA 380 bp) on 1,5% agarose (Sigma, A9539-500G) gel with Ethidium bromide solution (Sigma ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20 ...
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...
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bioRxiv - Microbiology 2020Quote: Cultures reaching ≥ 1 × 105 cells·ml−1 had 35 ml of the 50 ml volume filtered for identification via 16S rRNA gene PCR onto 25 mm 0.22-μm polycarbonate filters (Millipore, Massachusetts, USA). DNA extractions were performed using the MoBio PowerWater DNA kit (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich). The PCR products were then ethanol precipitated ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic regions containing guide sequences are PCR amplified using paired primers with 25 uL 2x JumpStart Taq Polymerase Ready Mix (Sigma-Aldrich), 1.5 uL 10 uM primer pair (CropSeq_NGS_P7 ...
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bioRxiv - Cell Biology 2021Quote: ... the 5’ sequence of the native promoter and amino acids 1-443 of HEH1 were amplified via PCR (KOD polymerase, EMD Millipore) from genomic DNA using primers encoding the restriction sites BamHI and HindIII ...
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bioRxiv - Microbiology 2019Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... A 245-base fragment of the end of the mto2 coding sequence was amplified from cps1-191 (containing the mto2S338N mutation) with flanking homology to the pFA6a vector using the ExpandTM High Fidelity PCR system (Millipore Sigma). The vector backbone was amplified from pFA6a-GFP-KanMX6 using Phusion polymerase (New England BioLabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines including stable clones were routinely evaluated for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... Ubi:caSMAD3 was cloned by PCR amplifying human constitutively active SMAD3 from Smad3 pCMV-SPORT6 (Harvard Plasmid Repository #HSCD00339271, Boston, MA, USA)(Sigma 11732641001), gel purifying (Qiagen #28604 ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were then extracted and used as template in ~400 bp NGS amplicon generation PCR reactions using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The amplicons were gel-extracted using the Monarch DNA Gel Extraction kits (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Indexing PCR reactions were pooled prior to magnetic bead clean-up with Sera-Mag SpeedBeads ™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that are likely to represent contamination from fresh DNA and libraries were selected with a bead concentration of 1.8X to size select the expected library range of ~300 bp ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... a first PCR was performed using the templates and primers shown in Table 1 with KOD hot start DNA polymerase (Sigma-Aldrich), with annealing time of 30 seconds at the indicated temperature ...
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bioRxiv - Plant Biology 2022Quote: Conserved regions were amplified in a standard Polymerase Chain Reaction (PCR) using genome-specific primers (Linares et al., 1998; Liu et al., 2019) synthesized commercially (Sigma-Aldrich). These probes were labelled with digoxigenin-11-dUTP ...
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bioRxiv - Neuroscience 2022Quote: ... The genomic region surrounding the CRISPR target site for SOX21 was amplified by PCR (KOD Xtreme Hot Start DNA Polymerase; EMD Millipore), and products were treated with SURVEYOR nuclease (SURVEYOR Mutation Detection Kit ...
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bioRxiv - Neuroscience 2023Quote: ... and fed with StemFlex media (ThemoFisher Scientific) and undergo monthly mycoplasma testing to ensure cultures are mycoplasma-free with the LookOut Mycoplasma PCR detection kit (Millipore Sigma). For the differentiation of iPSC-derived microglia-like cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines tested negative for mycoplasma contamination using a PCR-based method using the VenorGeM Classic Mycoplasma Detection Kit (Sigma-Aldrich). STR-genotyping was performed to confirm the identity of HeLa-JVM ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed following a standard procedure: The reaction tubes were prepared by adding 10 µL of RED Extract (Sigma-Aldrich), 4 µL MilliQ water ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were confirmed to be free of mycoplasma contamination (VenorTMGeM Mycoplasma Detection Kit, PCR-based, Sigma Aldrich Cat #MP0025). Cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR reaction mixtures were set up with 10 µl of SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich), 2 µl primers (final concentration 1 µM) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Genetics 2023Quote: ... Our method of genotyping involved PCR amplification across mutation sites using the primers described in Table 1 (purchased from Sigma Aldrich). Then ...
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bioRxiv - Genomics 2023Quote: ... Homology sequences of 600-900bp flanking the cut site of C-terminal Yy1 were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Sigma Aldrich). DNA sequence encoding the C-terminal 3xT7-2xStrepII-FKBP12F36V tag was amplified from a plasmid gifted by R ...
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bioRxiv - Synthetic Biology 2023Quote: ... which can be PCR amplified (Phanta Max Super-Fidelity DNA Polymerase, Vazyme) with a set of primers (ordered from Microsynth or Sigma-Aldrich) to add a unique variable linker ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µL of diluted cDNA was mixed with 12,5 µL ReqTaq® Ready Mix™ PCR reaction mix (Sigma Aldrich, #R2523), 0,5 µL forward primer (0,2 µM) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from mice ear tissues (or tail for embryos) using Extraction « Extract-N-Amp PCR » kit (Sigma #XNAT2-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR1 products for each tumor were individually column-purified with a GenElute™ PCR Clean-Up Kit (Sigma-Aldrich NA1020-1KT). For PCR2 ...
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bioRxiv - Cancer Biology 2023Quote: cDNA was diluted 10-50 times and quantified by real-time PCR with LightCycler® 480 SYBR Green I Master (SIGMA). The RPL11 ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were regularly tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, CA).
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bioRxiv - Microbiology 2023Quote: ... The cassette corresponding the DHFR-pSAG1-TgAPT1-GFP cassette was amplified by PCR from the plasmid of the same name using primers ML3088/ML3089 with the KOD DNA polymerase (Sigma-Aldrich). This donor sequence was then used to transfected to Pru background ...
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bioRxiv - Developmental Biology 2023Quote: ... Truncated FBF expression constructs were generated by PCR from full-length FBF-1 or FBF-2 and inserted into pETDuet-1 plasmid (EMD Millipore). Full-length C ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was then performed using 200 ng of 4C-template DNA with the Expand Long Range PCR kit (Millipore, 4829042001) under specific PCR conditions (94 °C for 2 min ...