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Citations for Millipore Sigma :
4551 - 4600 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: 40000 cells/well were plated on 96-well plates (SPL Life Sciences Co., Korea) pretreated with poly-L-lysine (Sigma-Aldrich, Burlington, MA, USA). HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... five wood fragments were plated onto each of two plates of Malt Extract Agar (MEA) amended with 0.35 g l−1 of streptomycin sulphate (Sigma-Aldrich, St. Louis, MO, USA) (MEAS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 104 cells per spheroid were plated in 96 well V-bottom plates that had been coated twice by air-drying 25 µl of 20 mg/ml polyhydroxyethylmethacrylate (poly-HEMA; Sigma-Aldrich, St. Louis, MO) in 95% ethanol in each well ...
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bioRxiv - Neuroscience 2024Quote: ... The iL3 was then transferred in a single droplet to a 2% Nematode Growth Media (NGM) agar plate [67] and paralyzed with nicotine (Sigma N3876, 1% in dH2O). The iL3 was then scored for activation based on the presence (activated ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were isolated and maintained for 5-12 DIV on 35 mm coverslips (3.5×105 cells/plate) coated with poly-L-lysine/laminin matrix (Sigma-Aldrich, St. Louis, MO, USA). Primary neurons were grown on Neurobasal medium (Gibco BRL ...
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bioRxiv - Immunology 2024Quote: ... was set up by picking a fungal colony from YPD agar plates (50 g/L YPD broth powder [Sigma-Aldrich], 2% Agar [MP Biomedical]) and resuspending in 3 mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we filled each well of a 96 deepwell plate (1mL, Polypropylen; #135702; Dutscher, Bernolsheim, France) with 250µL of 60% (v/v) Glycerol (#G6279; BioXtra, ≥99% Sigma-Aldrich St. Louis, MO, USA) and eleven wells per strains (one strain per row ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The 96-well plates used for the 293T/ACE2 cells were coated with 50 μL of poly-l-lysine (Sigma-Aldrich, St. Louis, MO) at 50 μg/mL as previously described46 ...
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bioRxiv - Immunology 2022Quote: ... 2-4×104 bone marrow-derived DCs (BMDCs) were re-plated in 96-well round-bottom plates and stimulated with 1.0 μg/ml LPS (Sigma L2762: Escherichia coli 26:B6) and different concentrations of peptides for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... was set up by picking a fungal colony from YPD agar plates (50 g/L YPD broth powder [Sigma-Aldrich], 2% Agar [MP Biomedical]) and resuspending in 3 mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2023Quote: ... All well plates and culture flasks were coated prior to seeding with 7 µg/mL rat tail tendon collagen I (Sigma-Aldrich, St. Louis, MO) and rinsed with PBS 3 times ...
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bioRxiv - Cell Biology 2023Quote: ... Resuspended lipid extracts were diluted 1:10 in 96-well plates (Eppendorf twin tec 96, colorless, Z651400-25A; Sigma-Aldrich, St. Louis, MO, USA) prior to measurement ...
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bioRxiv - Biophysics 2023Quote: ... a Teflon spacer of 2 mm thickness was placed between the ITO-glass plates and the chamber was filled with a solution of 100 mM sucrose (Sigma Aldrich, St. Louis, USA) to hydrate the lipid film ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were maintained in culture at 37°C and 5% CO2 in 10cm plates with 10ml of media containing DMEM (#11995-065, Sigma-Aldrich, St. Louis, MO) and 10% FBS (#30-2020 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK-G551D-CFTR were seeded at 40000 cells/well into 384-well black clear bottom plates coated with 0.01% Poly-L-Lysine (Poly-L-Lysine, Sigma-Aldrich, #P8920, 0.1 % (w/v) diluted 1:10 in PBS- ...
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bioRxiv - Genetics 2023Quote: 96 well tissue culture plates (TPP, Switzerland) were coated with α-poly-L-lysine (MW 150 000-300 000, Sigma-Aldrich, St-Louis, MO) by pipetting 100 μL polylysine solution (0.5 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Neuroscience 2023Quote: ... striatal neurons were plated at a density of 50K per well onto 24 well plates or 20K per well onto 8 well chamber slides coated with poly-L-lysine (Sigma Aldrich, St. Louis, USA) (100 µg/ml) ...
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were plated in 48 well plates at 150,000 cells per well with 20 nM phorbol 12-myristate 13-acetate (PMA) (P8139-1MG ; Sigma-Aldrich, St. Louis, MO, USA) supplemented in RPMI-1640 media +10% FBS and +1% penicillin-streptomycin to induce differentiation to monocyte-derived macrophages (dTHP-1) ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and then incubated with diluted sera and then plates were washed and incubated with HRP conjugated anti-mouse IgG (SIGMA, St. Louis, MO, USA). Results were read at 450 nm to collect end point ELISA data ...
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bioRxiv - Microbiology 2023Quote: ... and rAgApyrase in the wells of a 96-well microtiter plate with a final volume of 0.1 ml containing 2 mM of either ATP (Millipore-Sigma, Burlington, MA, USA, #A26209) or ADP (Millipore-Sigma ...
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bioRxiv - Immunology 2023Quote: ... ∼2-5 x 106 cells were plated in 96 well U bottom plates and stimulated ex vivo with 30ng/ml PMA (Sigma-Aldrich, Cat# 79346-1mG) and 500ng/ml Ionomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... 1990] were used for cloning and transformants were selected on LB agar plates supplemented with ampicillin (100 µg/ml) (Sigma-Aldrich or Carl Roth). B ...
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bioRxiv - Cell Biology 2024Quote: ... SH-SY5Y cells were seeded at a density of 4.5 × 104 cells/cm2 in plates and differentiated with 10 μM retinoic acid (Sigma-Aldrich, St. Louis, MO, USA) for 72 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then heated on a hot plate at 80°C for 10 min in preheated (80°C) 80% formamide (Sigma-Aldrich, diluted in water), Alex488-tagged probes (a generous gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: McArdle RH7777 rat hepatocytes (McA cells) stably expressing human apoB100[31] were grown in culture plates coated with type I collagen from calf skin (Sigma Aldrich, St. Louis, MO) as previously described[32] and cultured in growth media composed of Dulbecco’s modified – Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... and plated on LB plates (25 g/L Luria-Bertani agar, Carl Roth, Karlsruhe, Germany) supplemented with carbenicillin 100 µg/mL (Sigma-Aldrich, St. Louis, USA). Plasmids were isolated from recombinant colonies and the identity of the inserted sequences was confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... We spread the 50 μl of the sample with a delta cell spreader on square agar plates with 10% TSB supplemented with filter-sterilized cycloheximide and filter-sterilized MBOA (200 mg/L, Sigma-Aldrich, St. Louis, USA). For 10 days we incubated the plates at room temperature (21 - 25 °C) ...
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bioRxiv - Cancer Biology 2024Quote: ... neurospheres were collected or attached to standard culture plates in media containing 5% FBS for 6 h and stained with crystal violet solution (Sigma-Aldrich, 548-62-9).
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bioRxiv - Genomics 2024Quote: ... Pre-differentiated cells were subsequently counted and plated at 400,000-450,000 cells per well of a 12-well plate coated with Poly-L-Ornithine in 1 mL of Maturation medium with 20 uM trimethoprim (TMP) (Sigma-Aldrich, Cat No. 92131) to activate the CRISPRa machinery in these cells (TMP stabilizes the degron-tagged CRISPRa machinery) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell cultures grown in 96 well plates were washed with PBS and incubated with a staining solution (5nM TMRM (Sigma-Aldrich #115532-50-8) and Hoechst were diluted in growth media and incubated for 45 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The arena was covered with a glass plate that was treated with Sigmacote® (Ref. SL2, Sigma-Aldrich Co. LLC, St. Louis, MO, USA) during experiments to prevent flies from leaving the arena and from walking on the ceiling ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant – less 300- 500 μL to avoid the pelleted material – was transferred using a multi-channel pipette into a 96- well filter plate (Millipore Sigma, cat. no. MVHVN4525). A 96-vacuum manifold (Millipore Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... and RT-qPCR was performed as described previously [48] using QuantiTect SYBR Green PCR kit and KiCqStart SYBR Green optimized primers from Sigma-Aldrich (KSPQ12012).
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bioRxiv - Genomics 2020Quote: ... 1-4 PCR wells were combined in a tube and 40 μL of 97% 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich #370533) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: DIG-labeled riboprobes were generated using 2ul of PCR template with the Roche DIG RNA Labeling Kit (T7) (Sigma aldrich, SKU 11277073910)
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bioRxiv - Neuroscience 2020Quote: ... Templates cDNA fragments for the synthesis of riboprobes were obtained by PCR using specific primer sets (Table 1) and subcloned into the plasmid pSTBlue-1 (Novagen, Madison, WI). Nucleotide sequencing revealed 100% homologies of these fragments to the reported sequences in Genbank ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested and with Nde1/Xho1 restriction enzymes and inserted into the bacterial expression vector pET-30a(+) (Novagen, Madison, WI) cut with the same enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... A PCR master mix was prepared by combining the following reagent volumes per sample: 15 µl of Platinum PCR Supermix UDG (Sigma Aldrich, USA), 0.25 µl each of Forward and Reverse Primers (10 µM ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded on 96-well PCR plate and run in CFX real-time PCR System (Bio rad) using KiCStart SYBR Green qPCR ReadyMix with ROX as recommended by the manufacturer (Sigma-Aldrich: KCQS02). Three technical replicates were performed for each sample together with no-template control (NTC ...
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bioRxiv - Microbiology 2022Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). All chemicals and antibiotics were obtained from Sigma unless otherwise noted.
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bioRxiv - Microbiology 2022Quote: ... 500 base pairs of upstream and downstream sequence surrounding PBP-lipo (Rv2864c and MSMEG_2584c) were amplified by PCR KOD XtremeTM Hot Start DNA polymerase (EMD Millipore, Billerica, MA). These flanking regions were amplified with overlaps for the zeocin resistance cassette ...
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bioRxiv - Cell Biology 2022Quote: The determination of telomerase enzyme activity was done in HEKn cells by using TeloTAGGG Telomerase PCR Plus Kit (Sigma Aldrich, USA, 12013789001) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.