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Citations for Millipore Sigma :
4501 - 4550 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed utilizing the S-GAG set of primers and probe (Cline et al., 2005)(Sigma-Aldrich, Burlington, MA) with a 1-step Master mix (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: ... Regions determined to be differential between the Illumina sequencing performed in 2017 and the PacBio sequencing performed in 2020 and 2021 were verified by polymerase chain reaction (PCR) using RedTaq (Sigma-Aldrich) according to standard protocols ...
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bioRxiv - Genetics 2022Quote: ... T4 ligations into multiple cloning site plasmids were performed by amplifying fragments via PCR with Roche Expand High Fidelity Polymerase (Sigma 11732641001) with primers containing 5’ restriction enzyme sequences for digestion following PCR purification ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: ... All the cell lines were regularly tested for mycoplasma contamination using the LookOut Mycoplasma PCR detection kit (Product no. #MP0035 Sigma-Aldrich) and maintained in humidified cell culture incubators at 37°C and 5% CO2.
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... The absence of mycoplasma contamination in the virus stocks was confirmed using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma; #MP0035).
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bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Microbiology 2020Quote: ... Five wood fragments were plated onto each of two plates of Malt Extract Agar (MEA) amended with 0.35 g l−1 of streptomycin sulphate (Sigma-Aldrich, St. Louis, MO, USA) (MEAS ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 100 L1 worms in M9 were dispensed to each well of a 96 well plate and an equal volume of potassium cyanide (KCN) (Sigma-Aldrich St. Louis, MO) solution was then added to a final concentration of 200 µM KCN ...
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bioRxiv - Cell Biology 2020Quote: ... and E-cadherin (MSD, F21YX) or α-SMA (custom made, high binding plate from MSD, L15XB, with α-SMA antibody from Sigma-Aldrich, A2547) were measured in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Lung homogenates were serially diluted and plated onto 7H11 agar plates enriched with OADC (oleic acid, albumin, dextrose, and catalase, Sigma-Aldrich, St. Louis, MO) and incubated at 37ºC for 3 weeks ...
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bioRxiv - Neuroscience 2021Quote: ... at a density of 40,000 cells/well in 24-well plates for EdU incorporation assays or on 35-mm tissue culture dishes coated with poly-L-lysine (Sigma, P1524; 30 μg/mL) at a density 10×106 cells/dish for immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells in 96-well plates were incubated in 100 μl of serum-free medium containing thiazolyl blue tetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MO) at a concentration of 0.5 mg/ml for 1–3 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... and transferred onto BIOLAMININ 521 LN (LN521, BioLamina, Sweden. Product# LN521-02) coated plates with E8 media supplemented with 10uM Rock inhibitor Y-27632 (Sigma-Aldrich, US. Product# SCM075). The following day (D0 ...
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bioRxiv - Cancer Biology 2019Quote: ... were attached to wells in 12-well (up to two inserts) or 24-well (one insert) plates pre-coated with poly-D-lysine for 15 minutes (Sigma-Aldrich, St. Louis, MO). ∼8×105 cells were seeded to each chamber of the insert in 100 µL resulting in cells at ∼80-90% confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured in triplicate in 96-well plates in the presence or absence of CS055 (Shenzhen Chipscreen Biosciences Ltd, Shenzhen, China) and Dox (Sigma, St. Louis, MO, USA) with the final volume of 100 μl ...
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bioRxiv - Genomics 2021Quote: ... U2OS cells in 6-well plates were transduced at 50% confluence with 200 μL of 10x concentrated lentivirus and 5 μg/mL polybrene (EMD Millipore TR-1003-G). Approximately 4 days after transduction ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... The plates were washed using ELISA washing solution followed by addition of substrate (4-Nitrophenyl phosphate disodium salt hexahydrate, Sigma-Aldrich, 1 mg/ml), 150 µl/well ...
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bioRxiv - Bioengineering 2021Quote: ... using a Milliplex Rat Gut Hormone Panel 96-well plate assay as described by the manufacturer (#RMHMAG-84K-06; EMD Millipore Corporation, Billerica, MA). Data was collected with MAGPIX with xPONENT software (Luminex ...
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bioRxiv - Cancer Biology 2021Quote: ... MM1.S and U266 myeloma cells were transduced with shRNA-eGFP-containing pLKO.1 lentiviruses in 24-well plates (10 x 104 cells per construct) and in presence of polybrene (Sigma; final concentration 8μg/ml). Medium was changed by centrifugation (5min ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 cm or 10 cm cell culture dishes or 96 well flat-bottom plates (Thermo Fisher Scientific, Germany; Sigma-Aldrich, Germany; TPP AG, Switzerland). For analyses using the Operetta high content imaging system cells were plated with 10,000-50,000 cells/ml in 96-well µClear plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml FGF9 and 2 µM ROCK inhibitor into Corning® Costar® Ultra-Low Attachment 96-well round bottom plates (Sigma #CLS7007-24EA). Surplus NPCs were frozen in CryoStor® CS10 (Stem Cell Technologies #7930) ...
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bioRxiv - Immunology 2022Quote: ... and 50 μM 2-mercaptoethanol (D10 medium) in 12 well plates in the presence of adenosine (0–1 mM) (Sigma, St. Louis, MO, USA); in the presence of each adenosine receptor agonist (0–10 μM ...
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bioRxiv - Cell Biology 2022Quote: ... washed in sterile H2O and sown on vertical plates with agar medium (1 % [w/v] sucrose, 2.2 g/l MS salts [Sigma/Aldrich], 0.8 % agarose, [pH 5.7]) in cultivation room under a photoperiod of 16 h light:8 h darkness and 23 °C and light intensity 110 µmol/m2/s.
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bioRxiv - Microbiology 2020Quote: ... Wells of a 96-well microtiter plates (Falcon) were filled with 100 μL of sterilized 1% (w/v) YM (Sigma, St. Loius, USA; M7504) or mannose along with 100 μL inoculant (n=4) ...
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bioRxiv - Genomics 2022Quote: ... cells were plated at a density of 0.5×106 cells per well in 12-well plates along with increasing volumes of a 1 to 10 dilution of the master pool viral supernatant and polybrene (EMD Millipore, 8 µg/mL). Cells were then incubated at 37°C overnight ...
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bioRxiv - Genomics 2022Quote: ... 70% - 80% confluent cells from one well of a 6-well plate were dissociated to obtain single cell suspension and then resuspended in freezing medium (10% DMSO (Sigma Aldrich, Cat. No. D2650), 20% FBS (Millipore ...
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bioRxiv - Biophysics 2022Quote: ... and plated on LB plates (SigmaAldrich Co., St. Louis, MO, USA, 52062) with kanamycin (Sigma-Aldrich Co., St. Louis, MO, USA, 70560-51-9). Growing cultures were sequenced (Sanger Sequencing ...
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bioRxiv - Biochemistry 2019Quote: ... Lipids were then dissolved in 15 µL Chloroform and 1 µL was spotted on a TLC plate (Silica gel on TLC Al foils, Sigma-Aldrich, St. Louis, MO). Lipids were resolved based on polarity in a developer solution containing ethylacetate and cyclohexane in a 2.5:1 ratio ...
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bioRxiv - Microbiology 2019Quote: ... and added to wells in a sterile 96-well microtiter plate containing two-fold dilutions of each peptide with 0.01% v/v acetic acid (Sigma Aldrich, St Louis, MO, USA) and 0.2% w/v bovine serum albumin (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... These animals were raised at 20°C throughout the experiment and transferred to nematode growth medium (NGM) plate supplemented with 250uM 5-fluoro-2′-deoxyuridine (FUDR; Sigma-Aldrich, St. Louis, USA) to prevent progeny production ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The diluted antigen (100μl) was coated to the wells of a Nunc-Immuno™ MicroWell™ 96 well solid plates (Sigma-Aldrich, MO, USA) and was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... glycerol stock containing the uropathogenic Escherichia coli strain UTI89 was used to inoculate a Miller Luria Broth (LB) agar plate (Sigma-Aldrich, St. Louis, MO). The plate was incubated for 24 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Approximately 150 L1 worms in M9 were dispensed to each well of a 96 well plate and an equal volume of potassium cyanide (KCN) (Sigma-Aldrich St. Louis, MO) solution was then added to a final concentration of 150-250 µM KCN ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were plated at a density of 1.5×107 cells per 15cm2 plate in DMEM media with 10% fetal bovine serum and 1% Pen/Strep (Thermo #11965-092, Sigma #F4135, Thermo #15140-122). Six HEK293T 15cm2 plates were used per viral preparation ...
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bioRxiv - Microbiology 2021Quote: ... leaf discs were placed in a 96-well plate containing 50 μL of distilled water and 50 μL of assay solution containing 20 mM Luminol (Sigma-Aldrich, St. Louis, A8511), and 1 mg/mL peroxidase (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Pellet was resuspended in DMEM/F12 media and plated at 250,000 cells/mL on 6-well plates that had been pre-coated with 100 mg/ml PDL (Sigma-Aldrich, cat # 27964-99-4) at 37°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... tissue culture plates or acid-washed and sterilized glass coverslips were coated with PLL (Sigma Aldrich, # P4707, 100 μg/ml for 1.75-2 h). The dorsal 1/5th of the spinal cord of E13 rat neural tubes were micro-dissected and quickly washed once in cold Ca2+/Mg2+-free HBSS ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at 1 x 105 per well in a 6-well plate (FALCON, Cat.no. 353046) clonal density in 2i culture medium (15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... A 25 mm flat-top plate was quickly lowered to form a 25 mm disk before gelation of the IPN and mineral oil (Sigma-Aldrich, St. Louis, MO) was applied to the edge of the IPN to prevent sample dehydration ...
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bioRxiv - Cancer Biology 2022Quote: ... Organoids were dissociated into single cells and seeded into 2D plates and infected with lentivirus plus 1 ug/ml polybrene (Sigma-Aldrich# TR-1003-G). Three days after the infection ...
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... and, 7 days after germination, were transferred to plates with fresh ½ MS medium containing 10 µM NPA (Naphthylphthalamic acid, (Sigma-Aldrich, St. Louis, MO) for 24h before shift to 10 µM IAA.
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... Expression of UB10pro>>amiR-TOR was induced via transferring seedlings to plates containing ß-Estradiol (10 µM, Sigma-Aldrich, St. Louis, MO in DMSO) for 24hrs.
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bioRxiv - Neuroscience 2022Quote: ... each well of a 6-well plate (Axion Biosystems, catalog no. M384-tMEA-6W-5) was coated with 1 mg/mL Poly-L-lysine (Sigma, catalog no. P2636-100MG). The next day ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from ∼100 mg of seedlings grown on MS agar plate using TRI Reagent® (Sigma-Aldrich, St Louis, MO, USA) or TS™ Plant Mini Kit (Taeshin Bio Science ...