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Citations for Millipore Sigma :
4401 - 4450 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... astrocytes were thawed and plated at 20,000 cells/well in MEM with 10% Horse Serum and 6.7% glucose onto 24 well plates (Nest Scientific, 70201) containing 25mm coverslips pre-coated with 1mg/ml of Poly-L-ornithine (Sigma-Aldrich, P4957-50ML) for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Plant Biology 2022Quote: ... exactly 5 fronds were transferred into each well of a six well plate containing Schenk and Hildebrandt basal salt media (Sigma-Aldrich, S6765-10L) (0.8% agarose ...
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bioRxiv - Genetics 2023Quote: Resistance to chronic oxidative stress was measured by transferring 30 pre-fertile young adult worms to 60 mm NGM plates containing 4 mM paraquat (methyl viologen, Sigma Catalog No. 856177) and 100 μM FUdR and seeded with concentrated OP50 bacteria (see (Senchuk et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: One day before plating, 6-well MEA plates (Axion Biosystems, M384-tMEA-6W) were coated with poly-D-lysine (Millipore Sigma P6407-5MG) in borate buffer ...
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bioRxiv - Genetics 2023Quote: ... UV-induced mutagenesis frequencies were obtained by dividing the number of colonies growing on synthetic plates without arginine and containing l-canavanine (Sigma, 30 mg/l) (i.e. ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 20% irradiated fetal bovine serum and 1x antibiotic-antimycotic and cultured on plates coated with 2% gelatin (Sigma, St. Louis, MO). Renal epithelial cells were maintained in DMEM/Hams F-12 50/50 medium (Corning ...
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bioRxiv - Microbiology 2023Quote: HeLa cells grown on glass coverslips in 24 well plates were pretreated with DMEM containing 30mg/ml of DEAE-Dextran (Sigma, St Luis, MO) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... the isolated ganglia were immediately transferred into a 24-well plate containing digestion solution (1 ml high glucose DMEM and 100 μl Collagenase Type IV (Sigma; C1889-50 mg) and incubated at 37°C for 40 min ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Cancer Biology 2023Quote: 4×103 SW756 or HeLa cells were cultured in medium according to (51) in 48-well plates coated with 1% low attached agarose (Sigma Aldrich, Taufkirchen, Germany). For heterospheroids 4×103 SW756 and 2×103 Th17 cells were used ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.000 freshly sorted MM13 and MM27 GFP+ and GFP- cells were seeded in 96 well-plates in triplicates and 10uM of BrdU (Sigma-Aldrich, Cat#B5002) was added to complete culture medium ...
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bioRxiv - Microbiology 2023Quote: ... monolayers in 24-well plates at 70% confluency followed by the addition of semisolid media containing 0.8% methylcellulose (Sigma #M0512. Viscosity 4,000 cP) DMEM containing 2% fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... and rAgApyrase in the wells of a 96-well microtiter plate with a final volume of 0.1 ml containing 2 mM of either ATP (Millipore-Sigma, Burlington, MA, USA, #A26209) or ADP (Millipore-Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Cell toxicity was measured in 96-well plates (∼5×104 cells/well) using a lactate dehydrogenase (LDH) cytotoxicity kit (Sigma-Aldrich, Cat # 11644793001). Absorbance was measured at λ=490 nm ...
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bioRxiv - Cell Biology 2023Quote: Saccharomyces cerevisiae was purchased from ATCC (Cat No. 201389) and cultured at 30°C in Yeast Peptone Dextrose (YPD) agar plates or liquid broth (Sigma-Aldrich, Y1500, Y1375). Macrophage capacity for S ...
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bioRxiv - Cancer Biology 2023Quote: ... and 500,000 cells were plated per well of a 48 well suspension plate and the viral supernatant dilution was added with 12μg/mL polybrene (EMD Millipore TR-1003-G). The plate was centrifuged at 600 g for 40 minutes at 32C followed by a 3-4 h incubation at 37C ...
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bioRxiv - Developmental Biology 2023Quote: An aliquot of sperm was aspirated into a droplet of NIM 0.1% PVA on the injection plate and eggs were transferred for injection into droplets of FHM (Millipore, Cat# MR-122-D) with 1% PVA in groups of 10-15 ...
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bioRxiv - Plant Biology 2023Quote: ... 200 μL of the fungal suspensions was aliquoted in clear 96-well flat bottom polystyrene tissue-culture plates (Greiner CELLSTAR®, Sigma, Burlington, U.S.A). Plates were incubated at 28°C ...
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bioRxiv - Microbiology 2023Quote: ... Each suspension was pipetted (20 μl) and spread using a sterile spreader on agar plate media (LB) using single-use L-shape spreaders (Sigma, cat no. Z723193) and cultured at 30°C for 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... Reconstituted cell lysate was incubated with the reporter for 15 min at 25 °C and transferred into 96-well half area black flat bottom plates (Sigma-Aldrich CLS3694-25EA). FRET was measured on a POLARstar Omega (BMG Labtech ...
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bioRxiv - Immunology 2024Quote: ... the apical surface was washed and transwells were inverted into fresh 24 well culture plates containing HBSS alone or HBSS containing 100 nM N-formyl-methionyl-leucyl-phenylalanine (fMLP; Sigma; uninfected positive control). Neutrophils were applied to the basolateral compartment and transmigration was allowed to proceed for 2h (H292 cells ...
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bioRxiv - Immunology 2024Quote: ... Following incubation, plates were washed with PBS-T [1xPBS (Cellgro Technologies LLC, Lincoln, NE) with 0.05% Tween 20 (Sigma-Aldrich, St Louis, MO)] and blocked using PBS (Cellgro Technologies LLC ...
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bioRxiv - Genomics 2024Quote: ... iPSC-derived hNSCs were detached from hESC-qualified Geltrex using Accutase (STEMCELL catalog # 07920) and transferred to plates coated with Poly-L-ornithine (PLO)/laminin (Sigma Aldrich #P4957, #L2020) at low seeding density ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated at a density of 3x105 cells/well in a 24-well plate and treated with either vehicle (sterile phosphate buffered saline [PBS]) or 10ng/mL of LPS (O111:B4 Sigma, L4391, Lot#0000185317) for 18 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on gelatin-coated plates in CDM-PVA (same as CDM-BSA, replacing BSA with 0.5 g PVA (Sigma-Aldritch, 9002-89-50)) and differentiated to lateral mesoderm lineage for five days ...
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bioRxiv - Physiology 2024Quote: ... 8.0x104 cells/well were seeded the night prior on a 96-well plate and treated with 5mM 2-deoxy-D-glucose 2h prior to the initial seahorse reading (2-DG; Millipore Sigma cat. D2134). ATP production rates from glycolysis (glycoATP ...
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bioRxiv - Microbiology 2024Quote: ... was used to filter (0.45 μm PES) the cultures and resulting filtrates were collected in a 2 mL high-volume collection plate (Millipore Sigma, cat. no. MVCPN0025).
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bioRxiv - Cell Biology 2021Quote: ... The cyclin D1-Flag plasmid was generated by PCR insertion of cyclinD1 into the p3XFLAG-CMV™-14 expression vector (Sigma). The XPack-GFP plasmid was generated by inserting GFP from pEGFP-N1 (BD Biosciences Clontech ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines used in this study were negative for mycoplasma contamination as determined with Lookout Mycoplasma PCR Detection Kit (Millipore Sigma). During the course of this study ...
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bioRxiv - Biochemistry 2022Quote: ... and 3% hydrochloric acid followed by sample pooling via centrifugation at 1,500 rpm for 2 minutes to the proteoCHIP funnel part for direct injection or transferred to 0.2 mL PCR-tubes coated with 1e-3 % Poly(ethylene glycol) (95172-250G-F, Sigma-Aldrich, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... Products from the initial PCR of these samples were then purified using the Ultrafree-DA centrifugal filter units (Millipore, Cork, Ireland); the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). A549 cells (ATCC CCL-185 ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... To construct pKD1 the NUP188 coding sequence was amplified by PCR and subcloned into p3XFLAG-CMV™-10 vector (Millipore Sigma) using the Gibson Assembly® Master Mix (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products with the coding sequence were cloned into NdeI/XhoI or SalI/XhoI sites of pET30a (+) vector (Novagen, Darmstadt, Germany), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... qRT–PCR was performed with the Bio-Rad CFX384 Real-Time PCR system using the SYBR Green Jumpstart Taq ReadyMix (S4438-100RXN; Sigma-Aldrich) with the following PCR conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Cell Biology 2021Quote: ... CDC50.3 and CDC50.4 were generated using a PCR fragment encoding the mAID–HA and the HXGPRT cassette produced using the KOD DNA polymerase (Novagen, Merck) with the vector pTUB1:YFP-mAID-3HA as template and the primers indicated in Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Microbiology 2020Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich). Catalytically inactive Mt2 (Mt2 C78A ...
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bioRxiv - Microbiology 2020Quote: ... The 350 pb PCR product was digested and ligated into chloramphenicol-resistant pACD4K-C vector provided by the manufacturer (Sigma-Aldrich). The resulting recombinant pACD4K-C vector was transformed into Escherichia coli DH5a by heat shock ...
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bioRxiv - Microbiology 2020Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) (Fig 4A) ...
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bioRxiv - Microbiology 2020Quote: ... Double-stranded DNA templates for each sgRNA were produced by template-free PCR with two partially overlapping oligos (PAGE-purified, Sigma-Aldrich). Where necessary ...
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bioRxiv - Neuroscience 2019Quote: cDNAs templates were synthesized from extracted total RNA using omniscript reverse transcriptase (Quiagen) and used to perform PCR amplification under the indicated conditions using Taq DNA polymerase (Sigma-Aldrich).
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bioRxiv - Plant Biology 2021Quote: Seeds were sown individually inside PCR tubes filled with 1X MS gel medium: 0.44% Murashige and Skoog (MS) Basal medium (Sigma-Aldrich, M5519), 0.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... All progenies of genetic crosses were verified by diagnostic PCR using KOD XTREME Hot Start DNA Polymerase (71975-3, EMD Millipore) or OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (M0488L ...
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bioRxiv - Systems Biology 2022Quote: ... and cDNA fragments were amplified by PCR according to the manufacturer’s protocol (GenomePlex Single Cell Whole Genome Amplification Kit, Sigma Aldrich WGA4). The amplified libraries were run on a bioanalyzer to assess quality (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Biochemistry 2022Quote: ... aeruginosa PAO1 served as a template for the amplification of the genes of interest via PCR using the KOD Xtreme polymerase (Novagen/Merck) and cloning primers containing the restriction sites (Eurofins Genomics) ...