Labshake search
Citations for Millipore Sigma :
401 - 450 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: 2′,7′-dichlorofluorescein diacetate (4091-99-0, DCFH-DA, Sigma) was used as a sensitive and rapid identification of ROS in response to oxidative metabolism ...
-
bioRxiv - Cell Biology 2023Quote: ... Fluorescent dye 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Sigma Aldrich) was used to quantify the total ROS ...
-
bioRxiv - Molecular Biology 2023Quote: ... containing DCF-DA (2’,7’-Dichlorofluorescin diacetate) (Sigma-Aldrich #D6883) at a final concentration of 10 μM ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 ng/mL interleukin-4 (IL-4, Sigma-Aldrich) and 0.5 μg/mL anti-CD180 (BD PharMingen) ...
-
bioRxiv - Systems Biology 2020Quote: ... paired SUSD2- and SUSD2+ cells were seeded 6-well plates at a density of 2×105 cells/well and decidualized when 90% confluent with 0.5 mM 8-bromoadenosine cAMP (8-bromo-cAMP; Sigma-Aldrich) and 1 µM medroxyprogesterone acetate (MPA ...
-
bioRxiv - Genetics 2019Quote: BzATP (2′(3′)-O-(4-Benzoylbenzoyl) adenosine 5′-triphosphate triethylammonium salt) was purchased from Millipore Sigma and ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were incubated for 5 min with a 4’,6-diamidino-2-phenylindole (DAPI) solution (Sigma) to stain cell nuclei ...
-
bioRxiv - Physiology 2024Quote: ... samples were incubated with 5 µg/mL 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS ...
-
bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were applied overnight at 4°C in a blocking buffer of 5% donkey serum (Sigma-Aldrich) at the following concentrations ...
-
bioRxiv - Molecular Biology 2022Quote: U2OS cultured at 37 °C/5% CO2 in 15 cm2 dishes supplemented with 4 μg/ml Doxycycline (Sigma) for 48 hours to induce exogenous SAE1/SAE2 expression ...
-
bioRxiv - Microbiology 2022Quote: ... RNA (5 μg) was enzymatically hydrolyzed for 4 h at 37 °C with benzonase (99% purity, Novagen 70664), bacterial phosphatase (ThermoFisher 18011015 ...
-
bioRxiv - Cell Biology 2023Quote: ... skin samples were digested at 4°C overnight in 5 mg/mL dispase II solution (#D4693 Sigma-Aldrich) in HBSS (#H8264 Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then bleached overnight at 4 C in methanol containing a 5% of hydrogen peroxide (Sigma-Aldrich). Rehydration was done by incubating the samples in methanol 60% ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then bleached overnight at 4 C in methanol containing a 5% of hydrogen peroxide (Sigma-Aldrich). Rehydration was done by incubating the samples in methanol 60% ...
-
bioRxiv - Immunology 2023Quote: ... samples were cut into thirds and incubated overnight at 4°C in 5 mg/mL Dispase II (Sigma) in Hanks Buffered Salt Solution (HBSS) ...
-
bioRxiv - Microbiology 2024Quote: ... and 12,000 × g for 5 minutes at 4°C using a Sigma 3K30 centrifuge (Sigma Laborzentrifugen GmbH, Germany), and the supernatant was collected ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains of P0 pups were kept in 4% PFA in 4°C overnight and then transferred to 4°C 30% sucrose (Sigma-Aldrich, S5016) with 0.01% sodium azide (Fisher ...
-
bioRxiv - Developmental Biology 2022Quote: ... RNAs immersed in Trizol were added to phase-lock heavy gel tubes (Brinkmann Instruments, INC., #955-15-404-5) and mixed with 100 uL of 1-bromo-3-chloropropane (BCP) (Sigma, #B9673), followed by room temperature incubation for 10 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... pregnant female mouse with E14.5 embryos was intraperitoneally injected with 5-bromo-deoxyuridine (BrdU, Sigma #B5002; 50 µg/g body weight) 1 hour before collecting the embryos ...
-
bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
-
bioRxiv - Cell Biology 2021Quote: ... before fixation for 5 hrs in humid conditions at 37 °C in 1.4% / 2% formaldehyde (F8775 Sigma) / acrylamide (A4058 Sigma) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and subsequently fixed overnight at 4°C in 4% paraformaldehyde (Sigma). Next ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBST ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed and resuspended in PBS 4%BSA with viability staining reagent 1μg/ml (7-AAD) 7-aminoactinomycin D (Sigma Aldrich). Stained cells were analyzed with a MACSQuant 10 (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dried protein hydrolysates were re-dissolved in 16µL HPLC grade pyridine (Sigma 270407) prior to derivatization with 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934 ...
-
bioRxiv - Plant Biology 2021Quote: ... 200 µl were evaporated and reconstituted in 70 µl TMS+pyridine (Sigma-Aldrich). Samples were derivatized for 0.5 h in 70°C.
-
bioRxiv - Cell Biology 2022Quote: ... metabolites were incubated with methoximation (Sigma, 20 µl, 20 mg/ml in pyridine) overnight followed by trimethylsilylation (20 µl of N,O-bis(trimethylsilyl)trifluoroacetamide reagent (BSTFA ...
-
bioRxiv - Microbiology 2023Quote: ... 100 μl of the supernatant was transferred and evaporated to dryness on a speedvac. A 2% methoxyamine solution was prepared by dissolving methoxyamine hydrochloride (Supelco, ref. 226904-5g) in pyridine (Sigma-Aldrich, ref. 270970-1L). 25 μl of the methoxyamine solution was added to each sample and left to react for 1.5 h at 37°C for methoximation of the hexoses ...
-
bioRxiv - Developmental Biology 2023Quote: ... For derivatization of polar metabolites frozen and dried metabolites from cell extractions were dissolved in 20 µL of Methoxamine (MOX) Reagent (2% solution of methoxyamine-hydrogen chloride in pyridine; Cat. No. 89803 and 270970; Sigma-Aldrich; St Louis, MO) and incubated at 30°C for 90 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... ≥95% pure 2’-C-methylcytidine (2’CMC; Sigma, M4949) was serially diluted in 0.5 log10 increments in Milli-Q H2O ...
-
bioRxiv - Plant Biology 2019Quote: 7-Diethylamino-4-methylcoumarin (Sigma D87759-5G, 50 mg/ml in DMSO)
-
bioRxiv - Molecular Biology 2023Quote: ... 7 mg/ml α-cyano-4-hydroxycinnamic acid (CHCA; Sigma, MO, USA) added to 50% acetonitrile was applied with an HTX M5 sprayer.
-
bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
-
bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... the supernatant was incubated for 1.5-2 h at 4 °C with Anti-FLAG M2 affinity gel (Sigma) while gently mixing ...
-
bioRxiv - Microbiology 2019Quote: ... at 4°C and resuspended in 2 ml of NP40 lysis buffer (1× CellLytic B (Sigma, MO, USA), 50 U DNAse I (Fermentas ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were incubated 2 minutes at 4°C with 0.1 mg/ml propidium iodide (Cat. P4170, Sigma Aldrich) and fluorescence pulses were detected using 585/40 nm collection filter ...
-
bioRxiv - Molecular Biology 2023Quote: ... Rie1-3V5 was IPed at 4°C 2 h using 20 µL of anti-V5-agarose slurry (Sigma). After incubation ...
-
bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 2 hours at 4 °C and then incubated with anti-Flag M2 affinity gel (Millipore Sigma # A2220) for 1.5 hours at 4 °C under continuous rotation ...
-
bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
-
bioRxiv - Cell Biology 2023Quote: – 5-iodo-2’-deoxyuridine (IdU) and 5-Chloro-2′-deoxyuridine (CldU) (Sigma-Aldrich) were dissolved in sterile DMEM at 2.5mM and 200mM respectively and stored at –20°C ...
-
bioRxiv - Neuroscience 2020Quote: ... 5 mM 5-Fluro-2’-deoxyuridine (SIGMA U3003). Cells were transfected at DIV17-19 with Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... and 5’-fluoro-2’-deoxyuridine (Sigma, 5 μM) were added to the culture medium at days in vitro (DIV ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 mM 5-Fluro-2’-deoxyuridine (SIGMA U3003). Cells were transfected at DIV17-19 with Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
-
bioRxiv - Immunology 2020Quote: ... mosquitoes were kept at 26.0°C (± 0.5°C) in an ultrasonic humidity cabinet and provided with 8 % Fructose and 0.05 % 4-Aminobenzoic acid (both Sigma) feeding solution from this point forward ...