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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and 4 µM 5-fluoro-2′-deoxyuridine (FldU, Sigma-Aldrich) were added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: The bands were visualized using an acid phosphatase-conjugated secondary antibody and nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Sigma-Aldrich, St. Louis, USA) substrate system ...
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bioRxiv - Bioengineering 2024Quote: ... Phosphatase activity was determined by a chromogenic reaction using 5-bromo-4 chloro-3 indolyl phosphate and nitroblue tetrazolium (Sigma, St. Louis, MO, USA) as substrates.
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bioRxiv - Cancer Biology 2020Quote: ... cells were seeded in four chamber slides for 24 hours followed by incubation with 10 μg/mL 5- Bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich, Missouri, USA) for 1 hour (HCT116 ...
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bioRxiv - Bioengineering 2020Quote: ... finally various concentrations of HMGB1 (1-100 ng/ml) and a HMGB1 (1000 ng)-containing AS scaffold with 10 μM of 5-bromo-2’-deoxyuridine (BrdU; Sigma, St Louise, USA) were added into 1 ml of serum-free medium and cultured for additional 24 hours ...
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bioRxiv - Immunology 2021Quote: TCRδCreER.ZsGreen or TCRδCreER.TdTomato mice were fed with TAM chow for 30 days followed by normal chow and 1 mg/ml 5-bromo-2’-deoxyuridine (BrdU, Sigma, St. Louis, MO) in drinking water containing 1% glucose (to reduce the taste aversion and increase the palatability of water ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... The oxidation (5 equiv) was carried out with 0.05 M iodine in pyridine (BI0424-1005, Sigma-Aldrich). The detritylation step was performed using 3% dichloroacetic acid in toluene (BI0832-2505 ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... and 3 ug/ml 5-fluoro-2’-deoxyuridine + 7 ug/ml uridine (FRD+U; Sigma) added ...
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked overnight at 4 °C in 5% donkey serum (Sigma-Aldrich). For the proliferation study ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human lung fibroblasts of passage 5 until 7 (table 2) or CCL206 fibroblasts were proliferation-inactivated with mitomycin C (10 μg/ml, Sigma-Aldrich, St. Louis, MO, USA, #M4287) for 2 hours ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min and blocked for 2 hours at 25 °C or overnight at 4 °C using 2% BSA (Sigma-Aldrich). Samples were incubated with respective primary antibodies at 4 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... The antigen–antibody complexes were visualized using nitrotetrazolium blue chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) buffer (Sigma-Aldrich, St. Louis, Missouri, USA) under standard conditions ...
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bioRxiv - Genetics 2020Quote: ... and the colour reaction was carried out with NBT/BCIP (4-nitroblue tetrazolium chloride/bromo-4-chloro-3-indolyl phosphate; Sigma). Sections were dehydrated and mounted with Vectamount (Vector Laboratories ...
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bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for 1 hour at 37 °C in a sand bath ...
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bioRxiv - Physiology 2022Quote: ... Dried samples were suspended in 40 μL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5mM Bromo-UTP (Sigma B7166), 1% Na-laurylsarcosine ...
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bioRxiv - Cell Biology 2021Quote: ... pyridine-3-sulfonyl (PS) was purchased from Sigma. Pregnant Mare Serum Gonadotropin (PMSG)(110254564 ...
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bioRxiv - Neuroscience 2022Quote: ... in pyridine (20 mg/mL; Sigma-Aldrich #TS25730) to each pellet followed by 90 min dry heat incubation at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... in 10% (v/v) anhydrous pyridine (Sigma-Aldrich) and 90% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... in pyridine (20 mg/mL; Sigma-Aldrich #TS25730) followed by 90 min dry heat incubation at 30 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in 1 mL pyridine (anhydrous >99,8 %, Sigma Aldrich). After incubation at 30 °C and 700 rpm for 1.5 hours on a thermoshaker ...
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bioRxiv - Cell Biology 2023Quote: ... vinyl pyridine and triethanolamine were from Sigma-Aldrich. cmDCFDA (chloromethyl derivative of dihydrodichloroflourescin diacetate ...
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bioRxiv - Biochemistry 2024Quote: ... and 50 μL of pyridine (Sigma-Aldrich #270970) overnight (14–16 h ...
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bioRxiv - Biophysics 2020Quote: ... The cells were collected by centrifugation (6000 g, 5 min, 4 °C, Sigma 4-16KS tabletop centrifuge), lysed in lysis buffer (5 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... each mouse was intraperitoneally injected with 1 mg of 5-bromo-deoxyuridine (BrdU) (Sigma-Aldrich) each day for 3 days ...
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bioRxiv - Cell Biology 2023Quote: The immunostaining procedure for microtubules consisted of: fixation for 7 min at 37°C with 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... or 4-amino-5-phosphonooxymethyl-2-methylpyrimidine (HMP)(Sigma-Aldrich, USA), at the indicated concentrations for each of the experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and MRS 1220 (N-(9-chloro-2-furan-2-yl-[1,2,4]triazolo[1,5-c]quinazolin-5-yl)-2-phenylacetamide) were purchased from Sigma-Aldrich and dissolved in dimethyl-sulphoxide (DMSO) ...
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bioRxiv - Cell Biology 2019Quote: ... 7 µl 2-mercaptoethanol (07604, Sigma) and 690 µl Buffer B (50 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... NBQX (2 μg, Sigma, n=7), Bicuculline methiodide (0.05 μg ...
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bioRxiv - Plant Biology 2021Quote: 2’,7’ dichlorofluorescein diacetate (H2DCFDA, Sigma) was used to measure ROS in guard cells (49) ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg/mL 5-fluoro-2′-deoxyuridine + 7 μg/mL uridine (FRDU, F0503, Sigma-Aldrich). Cultured cells were then kept at 37°C and 5% CO2 in an incubator with culture media changes at 48 hours with supplemented media and NGF and FRDU until further experimentation.
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bioRxiv - Microbiology 2019Quote: ... for 5 min at 14,000 rpm at 4°C (Sigma 3-KIS centrifuge). Samples were snap-frozen in liquid N2 and delipidated by protein precipitation based on the protocol of Wessel and Flügge (61) ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were anaesthetized first with Halothane (2-Bromo-2-Chloro-1,1,1-trifluoroethane, Sigma-Aldrich, Germany; 1/1000 v/v), followed by a euthanasic dose of Dolethal (100 mg/kg of sodium pentobarbital ...
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...
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bioRxiv - Bioengineering 2021Quote: ... Bone marrow cells were cultured (7 d, 37°C, 5% CO2) in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma) enriched with 10% L929-conditioned media (containing macrophage colony-stimulating factor) ...
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bioRxiv - Cancer Biology 2020Quote: ... D9891), puromycin (Cat #: P8833), BrdU (5-bromo-2-deoxyuridine, Cat #: B5002) and polybrene (Cat #: TR-1003) were purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 sulfate was purchased from Gold Biotechnology (St ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and thus 3-[2-(diethylamino)ethyl]-7-hydroxy-4-methylcoumarin (AHMC; Cat. No. 188611, Sigma-Aldrich Corp.) was used instead as previously described for the fluorescence quantification (Donato et al ...
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bioRxiv - Neuroscience 2024Quote: ... Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2·5 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Cell Biology 2022Quote: ... in pyridine at 70°C for 15 min followed by addition of N-tert-Butyldimethylsiyl-N-methyltrifluoroacetamide (MTBSTFA, Sigma-Aldrich) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...