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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2 mM 4-aminopyridine (4-AP; Sigma) was added to the perfusate ...
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bioRxiv - Plant Biology 2020Quote: ... plants were incubated overnight at 37°C in GUS buffer (3 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide [Duchefa Biochemie, Haarlem, The Netherlands], 0.1% v/v Triton X-100 [Sigma, Steinhaim ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... 4–5-week-old mice were anesthetized by intraperitoneal injection of 2% 2,2,2-tribromoethanol (Sigma), dissolved in saline ...
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bioRxiv - Neuroscience 2023Quote: ... (D-Ala(2)-mephe(4)-gly-ol(5))enkephalin (DAMGO, Sigma-Aldrich, Cat. No. E7384) at 50 µM or lipopolysaccharide (LPS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed three times with PBS and further cultured at 37°C for 4 days in FBS-free DMEM supplemented with 0.5 μg/mL trypsin (Sigma Aldrich, 9002-07-7) until a clear cytopathic effect was visible ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed three times with PBS and further cultured at 37°C for 4 days in FBS-free DMEM supplemented with 0.5 μg/mL trypsin (Sigma Aldrich, 9002-07-7) until a clear cytopathic effect was visible ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was performed overnight at 4°C diluted in 5% BSA (Sigma-Aldrich, MO, USA) in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 500 g at 4°C for 5 min and resuspended in 1% formaldehyde (Sigma-Aldrich) for 20 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked overnight at 4°C with 5% skim milk powder/0.2% Tween 20 (Sigma Aldrich)/PBS solution (WB blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... then mixed with 5 μL of 4 mg/mL cytochrome c from horse heart (105201, Sigma-Aldrich). The mixtures were kept on ice for 1 hour with occasional vortex at high speed ...
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bioRxiv - Genetics 2019Quote: CEM and HBMEC cells were incubated overnight in complete culture medium containing 30 μM of 2-bromo-palmitate (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... Neurons were cultured at 37°C/5% CO2 and glial growth was inhibited at DIV4 by addition of 5-fluoro-2’-deoxyuridine (30 μM)(Sigma, F0503). A 50% fresh culture medium change was performed every 4 days.
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were blocked with 5% BSA diluted in PBS for 4 h at 4°C and incubated overnight with pan-Cadherin antibody (Sigma, C1821, 1:500), MRP2 antibody (Sigma ...
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bioRxiv - Immunology 2021Quote: ... After addition of 10 μl methoxyamine (20 mg/ ml in pyridine; Sigma), vials were agitated for 90 min at 34 °C and 750 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... 65 µL of Pyridin/methoxylamine (20 mg Methoxylamine/ 1 ml Pyridine; Sigma) was added to the tubes ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Bioengineering 2022Quote: ... cells were incubated for 2 h at 37 °C and 5% CO2 with MTT (Sigma-Aldrich) dissolved in DMEM media without phenol red ...
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bioRxiv - Immunology 2023Quote: ... inactive dephosphorylated A3 (2’,5’-A3) 53 or with poly(I):poly(C) (Millipore Sigma, #528906) at the concentrations indicated in figure legends with Lipofectamine 2000 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... after treatment for 2 h at 37°C with 5 μg/ml brefeldin A (Sigma-Aldrich). Cells were washed and fixed with BD cell fix diluted 4X in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Total DNA was labeled with 2 μg/ml of 7-AAD (7-amino-actinomycin D, Sigma-Aldrich) diluted in PBS containing 100 μg/ml of RNAse A (Sigma -Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... a freshly prepared solution of 5-(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; Sigma-Aldrich Co., St. Louis, MO, USA) in DMSO ...
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bioRxiv - Cell Biology 2023Quote: LPS- and OGD-treated cells were washed with PBS and stained with 5 μM 2′,7′-dichlorofluorescin diacetate (DCFH-DA; Sigma-Aldrich) for 20 min at 37°C to detect cell reactive oxygen species (ROS) ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... and stained with 200 μM 7-Azido-4-Methylcoumarin (AzMC) (Sigma). The stained cells were mounted on a glass slide and visualized using Leica TCS SP5 confocal microscope (excitation ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μM 5-Aza-2′-deoxycytidine (5-Aza; Sigma), 250-500 nM CpG-scramble ...
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bioRxiv - Microbiology 2019Quote: ... metabolic activity by the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetrazoliumhydroxidand (XTT; Sigma-Aldrich, USA) reduction assay ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... and stained overnight at 4°C with 2 µg/ml Hoechst 33342 (Sigma Aldrich, St. Louis, MO) and a 1:1000 dilution of anti-phospho-histone H3 (pHH3 ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% NP-40) at 4°C for 2 h with gentle rotation in a siliconized microtube (Millipore). Afterwards 25 µl of Protein A/G magnetic beads (Thermo Fisher ...
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bioRxiv - Bioengineering 2019Quote: ... mixed with 1-bromo-3-chloropropane (Sigma Aldrich, MO). Centrifugation was performed after 10 min of incubation for 10 min at 10.000 × g ...
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bioRxiv - Genomics 2019Quote: ... 200µl BCP (1-bromo-3-chloropropane) (B9673; Sigma Aldrich) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1 ml 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) was added to the homogenate and centrifuged for 15 minutes at 12,000 x g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 mM 8-bromo-cAMP (Sigma-Aldrich, Cat# B7880), and 0.1 mM IBMX (3-isobutyl-1-methylxanthine ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μM 4-hydroxy tamoxifen (4-HT; Sigma) was added at the time of activation.
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bioRxiv - Systems Biology 2022Quote: ... Fenofibrate (2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester) (Sigma # F6020) was injected peritoneally (4% DMSO/PBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 × 10−5 M 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2019Quote: ... injected daily with 5 mg/kg body weight ethyl-2-[6-(4-chlorophenoxy)hexyl]-oxirane-2-carboxylate (etomoxir) (Sigma-Aldrich, US), dissolved in 5% (2-Hydroxypropyl)-β-cyclodextrin solution from day 8 following tumor transplantation (LLC-Eto) ...
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bioRxiv - Microbiology 2020Quote: ... and 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥97%, Sigma-Aldrich) were of analytical grade and used as received ...
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bioRxiv - Pathology 2022Quote: ... by using 2′,7′-dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich, USA) as described in Yuan et al ...
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bioRxiv - Microbiology 2023Quote: Stock solutions of 2’,7’-dichlorofluorescin diacetate (DCF-DA; Sigma) were prepared by dissolving DCF-DA at a final concentration of 5 mM in absolute ethanol (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... MHA medium with 2 % dextrose (Sigma Aldrich, #50-99-7) and 0.5 μg/mL methylene blue dye (Sigma Aldrich ...