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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... embryos were fixed overnight at 4°C in 4%PFA-MFSW (Millipore-Filtered Seawater buffer containing 4% paraformaldehyde) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 30 ml of cold (4 °C) 4% paraformaldehyde (Sigma Aldrich) dissolved in 0.1 M phosphate buffer (pH = 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... with 7·5/1·875 mg/kg levodopa/carbidopa (D9628/C1335, Sigma), 0·15 mg/kg ropinirole (R2530 ...
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bioRxiv - Neuroscience 2020Quote: ... 5-7 dpf larvae were anaesthetized in 0.01% chilled tricaine (Sigma-Aldrich) and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar ...
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bioRxiv - Microbiology 2021Quote: ... samples were methoximated by resuspending the dried extract in 20 μl pyridine (Sigma 270970) containing methoxyamine-hydrochloride (20 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... samples were derivatised with 25μl 3% (w/v) methoxyamine in pyridine (Sigma, #226904/270970) for 60 min at 37°C with mixing at 750 rpm ...
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bioRxiv - Physiology 2022Quote: ... This then underwent cholesterol derivatization via sialylation reaction with pyridine (Sigma, 270970-100 mL) and N,O-Bis(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (BSTFA+TMCS ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Pellets were solubilized in 30 μl pyridine containing methoxyamine-HCl (10 mg/mL, Sigma) by sonication and vortex ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were thawed at 37 ºC and incubated in darkness with 4 μg/ml Hoechst (HO33342; 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate, Sigma-Aldrich, St Louis, MO, USA) DNA stain in solution buffer (1 M NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared by centrifugation and incubated at 4°C for 1.5–2 h with anti-FLAG M2 affinity gel (Sigma). After extensive washing with native IP buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary acinar cells were pelleted (80 g, 2 min, at 4 °C) and re-suspended in Waymouth media (Sigma). To obtain acinar-cell-conditioned media ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 xg at 4°C for 2 min and Pah1-FLAG was immunoprecipitated with anti-FLAG agarose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were incubated for 2 h on a rotating wheel at 4°C with anti-FLAG M2 beads (Sigma). Beads were washed once with lysis buffer (without benzonase ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 2 days at 4 °C in 0.1M of HCl digestion solution containing 1 mg/ml pepsin (Sigma). Collagen content was measured using the Sircol collagen assay kit (Biocolor) ...
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bioRxiv - Immunology 2023Quote: ... Serum and supernatant from homogenized tissues were incubated overnight at 4°C with 2% Trichloroacetic acid solution (Sigma, #T0699) at a 1:1 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and resuspended in 1-1.5 ml chilled wash and resuspension buffer containing 2% Bovine Serum Albumin (Sigma) and 0.2 U/μl RNase Inhibitor (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was incubated for 2 h at 4 °C with 150 μL streptavidin-agarose beads (Sigma-Aldrich) under gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... containing 5% 2-mercaptoethanl (Sigma) by incubating the beads at 100°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% 2-mercaptoethanol (Sigma) and fractionated by SDS-PAGE using the Mini-PROTEAN Tetra System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol (Sigma), and incubated at room temperature instead of boiled ...
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bioRxiv - Neuroscience 2022Quote: Larvae (24 hpf to 7 dpf) were fixed in 4% paraformaldyehyde (PFA; Sigma) for one hour shaking at room temperature (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.4) supplemented with 5mM 4-chloro-7-nitrobenzofurazan (NBF-Cl, Sigma-Aldrich), as described (Zivanovic et al 2019) ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 μM phytic acid dipotassium salt (Sigma, 5681, CAS: 129832-03-7) for the indicated time.
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bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Microbiology 2019Quote: ... J774 murine macrophage-like cells were cultured for a minimum of 4 passages in T75 tissue culture flasks at 37°C 5% CO2 in DMEM (High glucose, Sigma) supplemented with 10% Fetal Bovine Calf Serum (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4°C overnight with the following primary antibodies in 5% BSA in TBST: puromycin (Sigma, 1:25,000) and vinculin (Cell Signaling Technology ...
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bioRxiv - Genomics 2021Quote: ... Samples were then centrifugated at 500g for 5 minutes at 4°C and then were resuspended in 1mL Nuclei PURE Storage Buffer (Nuclei PURE storage buffer, Sigma). Sample washing was performed until the supernatant cleared ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were incubated O/N at 4°C followed by two washing steps of 5 minutes with Wash Buffer A (Sigma) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The fluid was centrifuged at 500xg for 5 min at 4°C and resuspended in RBC lysis buffer (Sigma, R7757) for 5 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the cells were firstly cultured for 4 h in a 37°C/5% CO2 incubator with 50 ng/ml PMA (Sigma) and 1 mM ionomycin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei and other debris were pelleted at 2,000 g for 5 min at 4°C and the supernatant was filtered through three layers of 100 μm pore nylon mesh (Millipore), and a 5 μm pore PVDF syringe filter (Millipore) ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... RT complete IntestiCult containing 25μg/ml was added, and enteroids were incubated (37°C, 5% CO2) until fixation with 4% paraformaldehyde (PFA; Sigma Aldrich) at 4h p.i ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... The conditioned media was spun down for 5 minutes at 800g and 4°C and then filtered with a syringe and 0.22 μm filter (Millipore, SLGV033RS) and stored at 4°C for no more than three days before being applied to cells ...
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bioRxiv - Molecular Biology 2023Quote: The sorted cells were concentrated by centrifugation at 300 x g for 5 minutes at 4 °C and resuspended in DPBS (Sigma) with 0.04% w/v BSA (Sigma) ...
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bioRxiv - Systems Biology 2023Quote: ... All membranes were blocked in a 5% milk solution and then incubated for 12h at 4°C with either the mouse anti-phosphotyrosine 4G10 (05321, Millipore) or the rabbit anti-GFP (Thermo A11122 ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The solubilized nucleoproteins were cleared by centrifugation at 20,400xg for 5 min at 4°C followed by filtration using an Ultrafree-MC spin column (Millipore, UFC30GV0S).
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bioRxiv - Cell Biology 2023Quote: ... The lysate was clarified by centrifugation at 20,000 × g for 5 min at 4°C and filtered on an Ultrafree-MC GV Centrifugal filter (Millipore, UFC30GV0S). Superose 6 Increase HR 10/300 column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... Embryos were then incubated overnight at 37°C under 5% CO2 in humidified air in 4-well culture dishes containing KSOM media (Millipore). Experiments were approved by the SIMR IACUC and were performed following the committees’ guiding principles.
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were reconstituted in 100 µL and 2 µL of the sample was injected from a 4 °C autosampler into a ZIC-pHILIC 150 × 2.1 mm 5 µm particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin was pelleted by spinning 5 minutes at 10,000 g at 4°C and subsequently incubated for 1 hour at 4°C in HEPES buffer containing 500 units of Benzonase (Millipore) and 2 µg Pol II antibody (ab5095 ...
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bioRxiv - Genomics 2020Quote: ... 5-aza-2’-deoxycytidine (5-aza-dC, Sigma, A3656) was diluted in DMSO at a concentration of 10mM and Abl.1 cells were treated using a concentration range of 10 nM to 20 μM 5-aza-2’-deoxycytidine ...